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Articles by W.M. Wan Yusoff
Total Records ( 3 ) for W.M. Wan Yusoff
  P. Abdeshahian , N. Samat and W.M. Wan Yusoff
  The production of β-glucosidase by Aspergillus niger FTCC 5003 from palm kernel cake as a substrate was studied in solid substrate fermentation using a laboratory column bioreactor. The simultaneous effects of three independent variables, namely incubation temperature, initial moisture content of substrate and airflow rate on the production of β-glucosidase were evaluated using response surface methodology on the basis of a central composite face-centered design. A total of 18 experiments were carried out in which Aspergillus niger FTCC 5003 was cultivated on PKC in an aerated column bioreactor for 7 days under incubation temperature, moisture level and aeration rate determined. Experimental results showed that the highest activity of β-glucosidase (52.06 U g-1) was obtained at an incubation temperature, an initial moisture level and an aeration rate of 32.5°C, 60% and 1.5 L min-1, respectively. Statistical analysis revealed that the quadratic terms of incubation temperature and initial moisture content had highly significant effects on the production of β-glucosidase (p<0.01). The statistical results also showed that the interaction effect between initial moisture level and aeration rate significantly influenced the production of β-glucosidase (p<0.05). Optimum conditions suggested by the second-order polynomial regression model for attaining maximum β-glucosidase production were 31.6°C incubation temperature, 57.0% initial moisture and 0.5 L min-1 aeration rate with a predicted production value of 47.20 U g-1.
  P. Abdeshahian , N. Samat and W.M. Wan Yusoff
  Aspergillus niger FTCC 5003 was cultivated on palm kernel cake as a substrate to produce β-xylosidase in solid substrate fermentation. The concurrent effects of three independent variables, including incubation temperature, initial moisture content of substrate and airflow rate on the production of β-xylosidase were studied by response surface methodology. A number of 18 trials were carried out in which solid cultivation of substrate was performed in an aerated packed-bed bioreactor for 7 days. Experimental results showed that the highest activity of β-xylosidase (6.13 U g-1) was obtained at an incubation, an initial moisture level and an aeration rate of 32.5°C, 60% and 1.5 L min-1, respectively. Statistical analysis revealed that the quadratic terms of incubation temperature and initial moisture content had highly significant effects on the production of β-xylosidase (p<0.01). Optimum conditions suggested by the statistical model for the production of β-xylosidase were 32.3°C incubation temperature, 59.7% initial moisture and 0.5 L min-1 aeration rate for attaining a predicted production level of 5.32 U g-1.
  A.M. Mukred , A.A. Hamid , A. Hamzah and W.M. Wan Yusoff
  Addition of nitrogen sources as supplementary nutrient into MSM medium to enhance biodegradation by stimulating the growth four isolates, Acinetobacter faecalis, Staphylococcus sp., Pseudomonas putida and Neisseria elongata isolated from petroleum contaminated groundwater, wastewater aeration pond and biopond at the oil refinery Terengganu Malaysia was investigated. The organic nitrogen sources tested not only supported growth but also enhances biodegradation of 1% Tapis crude oil. All four isolates showed good growth especially when peptone was employed as the organic nitrogen compared to growth in the basal medium. Gas chromatography showed that more then 91, 93, 94 and 95% degradation of total hydrocarbon was observed after 5 days of incubation by isolates Pseudomonas putida, Neisseria elongate, Acinetobacter faecalis and Staphylococcus sp., respectively.
 
 
 
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