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Articles by V. Devi Rajeswari
Total Records ( 6 ) for V. Devi Rajeswari
  Alok Prakash and V. Devi Rajeswari
  Knowing the appropriate applications of various analytical techniques used in biotechnology, makes the experiment more easier and let the researcher obtain a better result which also reduces the time period of experiment by eliminating the trial and error methods. Along with the knowledge of application of the analytical technique, the researcher also should have the knowledge of the sampling techniques for the analytical instruments as it is one of the deciding factors to choose a specific technique for the analysis. At the same time, the results obtained by using the analytical equipments are relatively authentic and accurate. With the development of biotechnology, the usage of analytical equipments has increased by many folds. The areas of biotechnology such as nanotechnology, cancer biology, genetic engineering and many more are dependent on the analytical equipments for most of their experimental analysis. Analytical equipments find its application not only in the research works but also at the industry level. Various food and pharmaceutical industries rely upon the analytical techniques like FT-IR and Raman for the quality analysis of the products.
  A.S. Vickram , V. Devi Rajeswari , M. Ramesh Pathy and T.B. Sridharan
  This is a preliminary study of the protein profiles of the semen in Jersey breed bulls. Jersey bulls were mainly used for fertility purposes; they were grouped based on the fertility, such as highly fertile group (95% fertility), medium fertile group (65-70%), low fertile group (45-50%) and hybrid bulls. Semen of 20 Jersey and hybrid bulls was collected through artificial vagina. The semen characteristics includes volume, pH value, viscosity, viability, sperm concentration, agglutination, total motility and their group, sperm morphology and Hypo-Osmotic Swelling test (HOS) were carried out immediately after collection. Total antioxidant capacity test by using catalase and cholesterol analysis were also carried out. Quantification of the amino acids and proteins were carried out by Ninhydrin and Lowry method, protein analysis by Sodium Dodecyl Sulphate (SDS) was carried out for the following categories. (1) Highly fertile group (Jersey), (2) Medium fertile group (Jersey), (3) Low fertile group (Jersey) and (4) Hybrid group. Analysis of semen protein reveals about seventeen protein bands of sizes ranging between 14 and 205 kDa were identified. Among those proteins bands, the relative protein content of nine bands shows significantly different from each group. The rest of the proteins bands seem to have some positive and/or negative correlation on the semen characterizes or fertility. The antioxidant capacity of highly fertile and hybrid group was very high compare to low fertile group of Jersey. This study clearly indicates the bull seminal plasma proteins are associated with fertility and as well as determination of semen quality and ultimately decides the fertility.
  V. Devi Rajeswari , G. Jayaraman and T.B. Sridharan
  In this study extracellular protease was purified with ammonium sulphate precipitation, dialysis, gel filtration and ion exchange chromatography. The purity was checked with MALDI-TOF and SDS-PAGE. Confirmation of protease was done with zymogram. The molecular weight of the protease was found to be 36 kDa. MALDI-TOF analysis of the protein showed the Molecular weight confirmation of the studied protease. Further the enzyme was characterized for its maximal activity with different pH, temperature, salt concentration and inhibitors. The maximal activity was found at pH 7.0, 40°C, 1.5 M salt concentration and found to be serine protease.
  A.S. Vickram , V. Devi Rajeswari , M. Srinivas , G. Jayaraman , R.A. Kamini , M. Ramesh Pathy , S. Ventat Kumar and T.B. Sridharan
  Purpose of this research was to elucidate the protein profiles of the seminal plasma in various categories of male infertility, to scrutinize their correlation with seminal parameters. Oligoasthenospermia (N = 15), asthenospermaia (N = 17), azoospermia (N = 12), normospermia (N = 27), oligospermia (N = 12) and fertile (control subjects, N = 10) were collected. The samples were diluted by tris-egg yolk extender and were frozen. Plasma was separated from semen by centrifugation, underwent SDS Polyacrylamide Gel Electrophoresis (SDS-PAGE). The mean values with its standard error of semen parameters of fresh sample were shown significant difference (p<0.0001) when compared to the post-thaw samples. Of the various fractionations, the protein with molecular weight 44.6 kDa shows high significant and positive correlation (p<0.01) with sperm concentration of freshly evaluated semen samples and low level significant (p<0.00001) with the frozen samples. Sperm motility was positively correlated (p<0.029) with the protein molecular weight 56.6 kDa in the freeze thawed semen samples. This reality could sustain the implication that seminal plasma proteins act on the sperm physiology and morphology and it found to act on strange ways. Supplementary studies are essential to define the mechanism of different proteins involved in the fertilization and their correlation.
  V.N. Kalpana and V. Devi Rajeswari
  In this study, the agricultural waste was used to screen for an organism that is capable of producing enzymes for degrading xylan. Streptomyces sp. are the important source of enzyme involved in lignocellulosic degradation. Streptomyces sp. was isolated from different soil samples. Out of 10 different soil samples, 6 samples gave the best result in starch casein agar plates. Screening was mainly carried out to detect the enzyme and a clear zone surrounding the growth was seen if enzyme xylanase was present. Substrates being cheap and readily available, have recently gained considerable interest because of their possible use in fermentation process. Streptomyces sp., produces xylanase on various feed stuffs like sugarcane molasses, oat spelt xylan, Tomato pomace, Rice bran, wheat bran and saw dust under submerged fermentation condition. The attempts have been made to replace a xylan, costly substrate for xylanase to make xylanase production cost effective. The xylanase activity in each production medium was confirmed by measuring the amount of reducing sugars liberated from the medium by the DNS method using crude extract. The application of the crude enzyme in deinking of newsprint was also studied.
  Haymanti Saha , Apoorva Srikkanth , Siddharth Sikchi and V. Devi Rajeswari
  The current investigation was performed with the objective of finding the scientific reason for health benefit of Occimum sanctum, Phyllanthus niruri and Cadaba fruticosa, simultaneously evaluating the antioxidant activity of ethanol, methanol and aqueous extracts. The potent antioxidant, antimicrobial and anti-inflammatory leaf extracts can be used for producing a novel ointment formulation, functional food or neutraceutical, in combination. The qualitative analysis of antioxidant activity was performed by reducing power assay and DPPH assay and in vitro anti-inflammatory test was performed by the HRBC membrane stabilization method. Further, the bioactive compounds present in the alcoholic leaf extracts were detected by GC-MS analysis and their applications were noted. Comparative studies on antimicrobial and anti-inflammatory activity of the 3 samples were determined using respective standards. Cadaba fruticosa is an under-emphasized herb, whose potential in the medical world is yet to be explored in details. Hence, Cadaba fruticosa has been chosen as one of the herbal plants for comparative study with Ocimum sp. and Phyllanthus sp. Ethanolic extract of showed significant antimicrobial activity against the tested bacterial strains. Methanolic extract of Ocimum sanctum at 200 Fg mL–1 depicted highest scavenging activity, 81.1%. The results also indicate that Cadaba fruticosa possesses significant free radical scavenging and anti-inflammatory activities in vitro. GC-MS analysis of the herbs revealed the presence of several bioactive compounds.
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