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Articles by Tomoyuki Nakagawa
Total Records ( 4 ) for Tomoyuki Nakagawa
  Viagian Pastawan , Yuny Erwanto , Lies Mira Yusiati , Jamhari , Takashi Hayakawa , Tomoyuki Nakagawa and Nanung Agus Fitriyanto
  Background: This study was performed to determine ammonia oxidation ability of the livestock farm waste by a microbial consortium and to reduce the amount of ammonia gas from the manure. Materials and Methods: The study consisted of five treatments: Without the addition of isolates as control, the addition of Pseudomonas sp., LS3K, the addition of Candida sp., LS3T, the addition of Arthrobacter sp., LM1KK and the addition of microbial consortium. The observed parameters consisted of microbial growth and the oxidation ability of the microbial consortium to minimize the concentration of ammonia. The obtained data were analyzed using one-way analysis of variance and the mean differences between treatments were tested by Duncan’s Multiple Range Test (DMRT). Results: The results showed that determined by Optical Density (OD), the microbial consortium was capable of growing with the addition of more than 10% ammonia with a value of maximum growth (μ max) 4.02 h–1. The number of colonies that grew on the addition of 0, 2.50, 5, 7.50 and 10% (NH4)2SO4 were 1.03×105, 0.94×105, 1.60×105, 0.82×105 and 0.86×105 CFU mL–1, respectively. The reduction of the ammonia concentration in the liquid medium with (NH4)2SO4 was 15.37 ppm. Ammonia emission from the chicken excreta, dairy cows feces and beef cattle feces was lower after the treatment with the addition of microbial consortium which was 28.72, 71.47 and 56.50 ppm, respectively compared than that of the control. Conclusion: The result can be concluded that the ability of oxidation by the microbial consortium was the most optimal in the oxidizing ammonia in beef cattle feces. The capability oxidizing of microbial consortium can reduce as much as 15.34 ppm concentration of ammonia in beef cattle feces.
  Nanung Agus Fitriyanto , Atik Winarti , Fatih Akbar Imara , Yuny Erwanto , Takashi Hayakawa and Tomoyuki Nakagawa
  Background and Objective: A proper management processes for safe and efficient handling of animal manure is important to minimize the possibilities of global environmental problem due to the emission of unpleasant odor. This study was performed to identify strains bacteria screened from the soil in the odorous region of apoultry format Yogyakarta city, Indonesia, to evaluate their capability for growing in ammonium and animal’s urine high content medium and also to investigate the ability to reduce ammonium concentration from the liquid medium. Materials and Methods: The strain is originally obtained from soil sample collected at ammonia high-emitted of chicken hen production area at tropical country Indonesia. The isolation was using a 1/100 nutrient agar with high concentration of (NH4)2SO4 as an organic ammonium stressor. Selected strain was then identified based on morphology and biochemical identifications, as well as molecular identification. The reduction of ammonium was measured from cultured medium using nessler reagent spectroscopically. Results: High ammonium sulfate-responsive, strain LS3K was successfully isolated and identified based on morphology, physiology and biochemical identifications, as well as molecular identification based on 16S rRNA sequence. Based on the morphology, physiological observation and the 16S rRNA gene sequence, strain LS3T was identified as Pseudomonas spp. The content of DNA G+C of the strain was 55.2%. The 16S rRNA gene sequence analysis demonstrated that the isolate is showing high similarity to species of Pseudomonas tolaasii. The cells of strain LS3K are short rods, aerobic and motile. Colonies (l-2 mm in diameter) on plate culture are yellowish, circular and smooth. This bacterium has an optimal growth temperature 30°C. Conclusion: Strain LS3K can tolerate ammonium at 756.77 mM and has the ability for growing in animal’s manure contained a medium that should make it applicable for use in animal waste handling systems as well as for enhancing the capability of deodorization. Ammonium was oxidized 5.648 mg L–1 by strain LS3K during 30 h cultivation period.
  Raden Lukas Martindro Satrio Ari Wibowo , Zaenal Bachruddin , Nanung Agus Fitriyanto , Tomoyuki Nakagawa , Takashi Hayakawa and Ambar Pertiwiningrum
  Background: Puffer fish skin tannery is an alternative to substitute the production of hide and animal skin in Indonesia that has been decreasing. To improve the quality of puffer fish leather, keratinase was needed by removing the thorns. Objective: The aim of this study was to screen bacteria that show keratinolytic activity. Methodology: The isolated bacterial strains were screened for the production of extracellular keratinase using skim milk agar. After incubation, the formations of a clear zone around the bacterial growth were observed. They were identified based on morphological enzyme activity and molecular identification. Bacterial screening and identification were conducted with descriptive method. The microbial activity tests were analyzed using a completely randomized design. Results: The result of the screening was that 3 of 5 kinds of strains exhibited caseinolytic activity (by showing the clear zone). Three Bacillus bacteria that were newly isolated from puffer fish waste-using a feather enrichment technique-were identified on the basis of 16S ribosomal RNA gene sequence analysis, physiological and carbohydrates assimilation tests. They were revealed as the strains of Bacillus thuringiensis for BRAW_PT isolate, Bacillus aerius for BRAW_PB isolate and Bacillus firmus for BRAW_PI isolate. The results of proteolytic enzymes assay showed that Bacillus firmus BRAW_PI has the highest protease and keratinase activity, which was 37.52±0.96 and 6.781±0.479 U mg–1 consecutively. Conclusion: All bacteria obtained were the superior bacteria that can be used for the removal of thorns from puffer fish skin in the tanning process.
  Nanung Agus Fitriyanto , Adhitya Permadi , Yuny Erwanto , Takashi Hayakawa and Tomoyuki Nakagawa
  Background: Animal manure may contributes global environmental problems especially unpleasant odor from the emission of ammonia. It challenges the development of proper management processes for safe and efficient disposal. Objective: The aim of this experiment was to identify isolates screened from soil in the odorous region of chicken hens farms in a tropical area and to evaluate their capability for growing in ammonium and animal’s urine high content medium. Materials and Methods: Indigenous isolates originally obtained from soil sample collected at ammonia high-emitted area of a tropical country Indonesia using a 1/100 nutrient agar with high concentration of (NH4)2SO4. Selected strain was identified based on morphology and biochemical identifications, as well as molecular identification (16S rRNA sequence homology). Reduction of ammonium was measured from cultured medium using Nessler reagent and spectrophotometer. Results: Ammonium sulfate-responsive, strain LM1KK was successfully isolated and identified based on morphology, physiology and biochemical identifications, as well as molecular identification based on 16S rRNA sequence. The cells of strain LM1KK are rods, aerobic and motile. Colonies (1-2 mm in diameter) on a plate culture are yellowish, circular and smooth. Based on the morphology, physiological test and its 16S rRNA gene sequence, strain LM1KK was identified as species Arthrobacter nitroguajacolicus. The 16S rRNA gene sequence analysis demonstrated this isolate is showing 98% similarity to species of Arthrobacter nitroguajacolicus MNPB4T. Conclusion: This bacterium has a higher optimal growth temperature of 30°C. Strain LM1KK can tolerate ammonium at high concentration and has the ability for growing in the urine contained medium that should make it applicable for use in deodorization plants for enhancing the efficiency of deodorization. Ammonium was oxidized 26.86 mg L–1 by strain LM1KK during 48 h cultivation period.
 
 
 
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