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Articles by Thongchai Taechowisan
Total Records ( 7 ) for Thongchai Taechowisan
  Thongchai Taechowisan , Suchanya Chaiseang , Winyou Puckdee and Waya S. Phutdhawong
  Background and Objective: Two new biphenyls, 3'-hydroxy-5-methoxy-3,4-methylenedioxybiphenyl (1) And 3'-hydroxy-5,5'-dimethoxy-3,4-methylenedioxybiphenyl (2) Have been isolated from Streptomyces sp. BO07, an endophyte in the root tissue of Boesenbergia rotunda (L.) Mansf A. Although, there are many biphenyls on anti-inflammatory activity have been reported. This study investigated the anti-inflammatory property of these biphenyls on LPS-induced macrophage RAW 264.7 cells. Materials and Methods: The effects of the two biphenyl compounds were investigated on the formation of NO, PGE2, TNF-α, IL-1β, IL-6, IL-10 and also on inducible nitric oxide synthase (iNOS) and cyclo-oxygenase-2 (COX-2) in LPS-induced murine macrophage RAW 264.7 cells. Results: The data obtained were consistent with the modulation of TNF-α, IL-1β, IL-6, IL-10 production by these biphenyls at concentration of 5-20 μg mL–1. A similar effect was also observed when LPS-induced NO release, iNOS and COX-2 enzyme expression were tested. The inhibitory effects were shown in concentration-dependent manners. However these compounds did not significantly reduce the formation of PGE2. Conclusion: From the obtained results, it is concluded that these biphenyls possess anti-inflammatory activity on LPS-induced RAW 264.7 cells.
  Thongchai Taechowisan , Kanticha Dumpin and Waya S. Phutdhawong
  Background and Objective: It has been previously reported that tea extracts of Camellia sinensis could inhibit the growth of influenza virus. The aim of the present study was to evaluate the effects of tea extracts on viral absorption and propagation. Materials and Methods: The H5N2 influenza A virus was isolated from a cloacal swab sample of an apparently healthy free-grazing duck in Banglane district, Nakhon Pathom province, Thailand in July, 2017. The extracts of dried tea leaves and green tea were used to evaluate the inhibition of influenza virus replication in embryonated chicken eggs. Results: The findings indicated that dried tea leaves extract and green tea extract inhibited hemagglutination caused by H5N2 influenza A virus and viral propagation and the amounts of total phenolic contents were correlated with inhibition of viral propagation. Conclusion: These results were expected to provide guides for rational design of tea extracts as antiviral substances to prevent influenza A virus infection, especially in pandemic area of avian influenza A viruses.
  Thongchai Taechowisan , Nantiya Chuaychot , Srisakul Chanaphat , Asawin Wanbanjob and Yuemao Shen
  Some endophytic actinomycetes (120) were isolated from the roots of Alpinia galanga. Identification of these endophytes was based on their morphology and amino acid composition of the whole-cell extract. Most isolates were classified as Streptomyces sp. (82), with the remainder belonging to Nocardia sp. (11), Microbispora sp. (3) and Micromonospora sp. (2). Eight isolates were unclassified and 14 were lost during subculture. The strain identified as endophytic Streptomyces sp. Tc052 strongly inhibited test microorganisms. This endophyte was cultured, the agar was extracted with organic solvent and the extract was purified on a column of silica gel to give a major component, which was identified to be kaempferol, isoscutellarin, umbelliferone and cichoriin on the basis of spectroscopic data. These compounds together with the extract were tested for their antimicrobial activity against bacteria and yeast using micro-dilution methods for the determination of Minimum Inhibitory Concentrations (MIC) and Minimum Microbicidal Concentration (MMC). The MIC values obtained with the crude extract varied from 64-128 μg mL-1 against tested microorganisms. All the isolated compounds showed various activities.
  Thongchai Taechowisan , Parisa Sarakoat and Waya S. Phutdhawong
  Background and Objective: Morinda citrifolia L. has been reported as a medicinal plant for treatment of abscesses and microbial infections. Thus, this study was conduced to isolate and identify the major constituents of the ethanolic extract of M. citrifolia fruit and also to evaluate their antibacterial, antioxidant and cytotoxicity properties. Materials and Methods: Isolation procedures included silica gel 60 column chromatography and thin layer chromatography. Identification of purified compounds was archived by spectroscopic methods. The antibacterial, antioxidant and cytotoxicity properties of purified compounds were carried out. Results: On the basis of the spectral data, the major compounds were isolated and identified as rutin (1) and asperulosidic acid (2) with yield of 9.28 and 7.71% (w/v), respectively. Antibacterial activity of crude extract and isolated compounds was evaluated by disc diffusion, MIC and MBC against Staphylococcus aureu, Bacillus subtilis, Escherichia coli, Salmonella typhimurium and methicillin-resistance Staphylococcus aureus SP6-106. The crude extract exhibited an antimicrobial activity at a dose level of 15 mg disc–1, while the isolated compounds exhibited an excellent antimicrobial activity at a dose level of 3.75 μg disc–1 in all test micro-organisms for disc diffusion method. Compound 2 showed the lowest MIC (8 μg mL–1) against S. aureus, while compound 1 showed the lowest MIC (16 μg mL–1) against E. coli. However, compounds 1 and 2 had high MIC values (512 μg mL–1) against B. subtilis. Compound 1 showed the lowest MBC (16 μg mL–1) against E. coli, while the compound 2 showed the lowest MBC (8 μg mL–1) against S. aureus whereas these compounds had high MBC values (512 μg mL–1) against B. subtilis. The compound 1 had the highest antioxidant activity with SC50 values of 68.45 μg mL–1, while compound 2 and the crude extract had antioxidant activity with SC50 values of 103.32 and 150.22 μg mL–1, respectively. The cytotoxicity activity of the crude extract and isolated compounds was observed and showed weak cytotoxicity activity with IC50 values of 1352.67-2780.80 and 1524.19-2854.06 μg mL–1 toward L929 and HEK293 cell lines, respectively. Conclusion: It can be concluded that the major compounds isolated from the crude extract of M. citrifolia fruits in Nakorn Pathom, Thailand were rutin and asperulosidic acid, which have antibacterial, antioxidant and cytotoxicity properties. They may provide promising improvements in the therapeutic approach to infectious diseases and oxidative stress treatments.
  Thongchai Taechowisan , Tipparat Samsawat , Winyou Puckdee and Waya S. Phutdhawong
  Background and Objective: Methyl 5-(hydroxymethyl)furan-2-carboxylate (MFC) was isolated as a major compound from Streptomyces zerumbet W14. This compound was evaluated the cytotoxicity and antibacterial activities. Materials and Methods: Isolation and structure elucidation of the bioactive compound were carried out using silica gel column chromatography and nuclear magnetic resonance. The cytotoxicity and antibacterial activities were reported. Results: MFC showed high antibacterial activity against Gram positive bacteria, Staphylococcus aureus, Bacillus cereus and Bacillus subtilis and moderate activity against Gram negative bacteria, Escherichia coli, Salmonella Typhi, Serratia marcescens and Pseudomonas aeruginosa. This compound showed bacteriolytic effects on the tested microorganisms, causing cell wall and membrane damage by using the Sytox Green assay. It showed moderate cytotoxic activity on both normal cells, LLC-MK2 cells and L929 cells with IC50 values of >512.00 and 239.06 μg mL1, respectively. The strongest cytotoxicity was observed in HeLa and HepG2 cells with IC50 values of 64.00 and 102.53 μg mL1, respectively. Conclusion: The findings revealed that MFC affects selective cytotoxicity on cancer cells and Gram positive bacteria at a low concentration. It suggested that MFC has a potential usage as an alternative agents for treatments of some cancers and some bacterial infections.
  Thongchai Taechowisan , Asawin Wanbanjob , Pittaya Tuntiwachwuttikul , Yuemao Shen and Saisamorn Lumyong
  Different extracts of Streptomyces aureofaciens CMUAc130 culture were studied as potential antifungal agents for selected phytopathogenic fungi. In a serial agar dilution method, crude ethyl acetate and 10% methanol in ethyl acetate extracts exhibited fungistatic activity against Aspergillus flavus, Colletotrichum musae, Fusarium oxysporum, Pythium ultimum, Rhizoctonia solani and Sclerotium rolfsii. Both ethyl acetate extract and 10% methanol in ethyl acetate extract were highly effective on all tested fungi, with Minimum Inhibitory Concentration (MIC) values ranging from 0.25 to 50 and 10 to 100 mg mL-1, respectively. The major active ingredients from those extracts were purified by silica gel column chromatography and identified to be 5, 7, 4`-trimethoxy-4-phenylcoumarin (1), 4`-hydroxy-5,7-dimethoxy-4-phenylcoumarin (2), 3`-Hydroxy-5,7,4`-trimethoxy-4-phenylcoumarin (3), 5,7,3`,4`-Tetramethoxy-4-phenylcoumarin (4) and 4`-hydroxy-5,7,3`-trimethoxy-4-phenylcoumarin (5) by NMR and mass spectral data, respectively. Five compounds (1 to 5) had activity against F. oxysporum with MICs of 0.30, 1.00, 0.40, 10.00 and 20.00 mg mL-1, respectively. Compounds 1, 2 and 3 also showed a synergistic effect when combined in different concentrations, displaying four times less concentration to reach complete inhibition in the growth of F. oxysporum.
  Thongchai Taechowisan , Nutthapol Mungchukeatsakul and Waya S. Phutdhawong
  Background and Objective: Staphylococcus aureus has long been recognized as one of the most important causes of bacterial disease in humans. This study investigates the prevalence of Staphylococcus aureus in clinical and environmental samples and their susceptibility patterns to antibiotics. Methodology: A total of 69 samples, 36 clinical specimens obtained from patients and 33 environmental samples from hospital facilities, were screened for S. aureus using standard microbiological and biochemical methods and PCR-based assay. Isolates resistant to both cefoxitin and oxacillin were considered to be Methicillin Resistant S. aureus (MRSA). Results: Of the two categories of samples screened, 31 (86.1%) and 23 (69.7%), respectively tested positive for S. aureus. The highest prevalence of MRSA from clinical samples (28.6%) was found in sputum and wounds and the highest (25%) from environmental samples was found in corridor, door handle and patient bed samples. Conclusion: In suggesting that healthcare personnel and hospital environments serve as potential reservoirs of S. aureus, these findings have practical, clinical and epidemiological importance. Ten of 16 of multi-drug resistant S. aureus isolates were MRSA, suggesting a correlation between the results of PCR patterns and their phenotypic multi-drug resistance testing.
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