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Articles by Thalisa Yuwa-amornpitak
Total Records ( 3 ) for Thalisa Yuwa-amornpitak
  Thalisa Yuwa-Amornpitak
  Ethanol production from cassava starch by co-culture of selected fungi and S. cerevisiae was investigated. Firstly starch hydrolysis fungi were isolated and screened from tan-koji (loog-pang). Enzymatic activities of 10 isolated Rhizopus sp. strains were determined on 0.1% starch agar plate at various pH (3-10) as primary screening. Clear zone diameter was occurred at pH ranging from 3 to 8 and no response at pH 10. The highest clear zone diameter that were found from 2 strains of Rhizopus sp. #2Bu and Rhizopus sp. #3Su at pH 4 were nearly equal. Secondary screening, sugar liberated were performed at various starch concentration from these 2 fungal strains. The results showed that Rhizopus sp. #3Su was the maximum efficiency. The highest reducing sugar yield was 25.9% from 6% cassava starch medium at 72 h. Ethanol production by SSF process, the coupling process between saccharification and fermentation was developed by using co-culture of Rhizopus sp. #3Su and Saccharomyces cerevisiae 5088. After 24, 48 and 72 h of saccharification period by the fungal strain, the fermentation process was begun by adding yeast inoculum. The highest ethanol production was achieved at 14.36 g L-1 after 24 h of saccharification process on 6% cassava starch medium.
  Thalisa Yuwa-amornpitak and Kannika Chookietwattana
  Lactic acid producing fungi were screened from soil samples at various temperatures. Several fungal strains were selected from 30 and 35°C and found that no fungal growth at 40 and 45°C. The results from starch hydrolysis and acid testing revealed that the highest lactic acid was produced by Rhizopus sp. LTH23. Effect of various parameters on lactic acid production were investigated. It was found that the enhancing temperature for higher lactic acid production was at 40°C from 5% starch medium and the medium should be adjusted to pH 9. Effect of N-sources on lactic acid production from NH4(SO4)2 and Urea at various concentrations were also studied. The results showed that more lactic acid production from the medium containing Urea than NH4(SO4)2. The optimum concentration of urea was at 7.5 g L-1. The effect of agitation speed on lactic acid production in batch fermentation showed that lactic acid of 20 g L-1 was produced during 60 h of cultivation at an agitation speed of 200 rpm, air flow rate of 0.5 vvm and maintained at a temperature of 40°C. Fed-batch fermentation system, a method for enhancing higher production yield was used. The result was indicated that lactic acid was produced 3 times higher than in the batch processing by adding 25% fresh medium at 10 h of cultivation. Fungal identification by ITS region sequence was used. It was proved that this fungus was 99.85% similar to Rhizopus microspores (Accession No. JX661044.1) from GenBank data base. Then named it as Rhizopus microsporus strain LTH23.
  Thalisa Yuwa-amornpitak , Taweerat Vichitsoonthonkul and Morakot Tanticharoen
  Dipterocarpoideae is a species-rich subfamily of Dipterocarpaceae. Dipterocarpaceae in Thailand is represented by 65 species in 8 genera. In recent years molecular taxonomist constructed a phylogenetic tree of dipterocarps found in South-East Asia. However, many species of dipterocarps found in Thailand are not included on that tree. In an effort to complete the tree, the phylogenetic relationships among 23 species belonging to 7 genera of dipterocarps found in Thailand were studies using nucleotide sequences of two intergenic spacer region between trnL-trnF and atpB-rbcL in chloroplast DNA. With Neobalanocarpus heimii as an out group, the molecular tree was consistent with the morphological classification and it was clearly resolved two major groups; Vavate-dipterocarpi and Imbricate-Shorea. The analysis showed that each member genera have similar characteristics and base chromosome number of 11 and 7, respectively. The first group consisted of genus Diptercarpus, Anisoptera, Cotylelobium and Vatica. Shorea and Hopea were in the second group.
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