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Articles by Tayyab Husnain
Total Records ( 6 ) for Tayyab Husnain
  Khurram Bashir , Muhammad Rafiq , Tahira Fatima , Tayyab Husnain and Sheikh Riazuddin
  In present studies, level of toxicity of hygromycin was optimized for local inbred line of maize (Zea mays L.). The immature embryos were isolated and cultured on N6 media modified with 1.0 mg L-1 2,4-D, 25 mM L-Proline and 100 mg L-1 Casein Hydrolysate and containing different concentrations of hygromycin. These immature embryos were observed for callus formation on working concentrations of 0, 20, 40, 60, 80, 100 and 120 mg L-1 hygromycin. The results showed that 97.3 38.5 and 32.5% calli survived on media containing 0, 20 and 40 mg L-1 hygromycin. Hygromycin concentration of 60 mg L-1 and above was effective for BR-6 as no callus formation was observed at this concentration or the immature embryos were completely dead. It was also observed that although 32.8% calli survived on media containing 40 mg L-1 hygromycin, these calli were slower in growth, smaller in size and pale in color as compared to calli containing (hph) gene on same media. On the basis of these results 40 mg L-1 hygromycin seems suitable for identification of putative transformants.
  Rozina M. Ali , Tayyab Husnain , Syed Sarfraz Hussain , Nasir Mahmood and Sheikh Riazuddin
  Induction of multiple shoots in Gossypium hirsutum L. variety CIM-443 has been achieved by using meristem and cotyledonary node as explants. Meristems of size 3-4 mm were excised from embryos isolated from seeds while cotyledonary nodes were cut from seven days old seedling and the mean shoot number per explant response for total of three experiments was maximum i.e., (6.72±0.79) and (4.92±0.67), respectively for both explants in MS medium supplemented with benzyl-amino purine (BAP) 1.0 mg L-1 + naphthalene acetic acid (NAA) 0.05 mg L-1. Shoot elongation was observed in MS medium amended with NAA 0.1 mg L-1. The percentage of shoots forming roots was maximum (79.16) in case of 1/2 MS supplemented with NAA 0.05 mg L-1. Rooted plantlets hardened in soil and normal boll formation observed.
  Syed Sarfraz Hussain , Tayyab Husnain and S. Riazuddin
  Successful cotton plant transformation depends on regeneration of plants from transformed cells. Recalcitrance of cotton to tissue culture has not only slowed the development of transgenic cotton but also narrowed its genetic base. Keeping this in view, an efficient in vitro plant regeneration system characterized by bulk, rapid and continuous production of somatic embryos using immature zygotic embryos, taken at various stages of development from ovules as explant has been developed in cotton. One of the drawbacks of using this is the requirement of large number of high quality immature zygotic embryos. To address this problem, we have developed a procedure that generates highly homogeneous populations of embryogenic calli by selectively propagating a small number of regeneration proficient calli derived from immature zygotic embryos. Stages showing the early cotyledon development cultured on modified Murashige and Skoog media proliferated intensely. Rapid callogenesis was observed from these immature zygotic embryos. To induce germination and plantlet growth, embryoids were placed on sterile processed cotton, saturated with Stewart and Hsu media. Upon development of roots and leaves, plantlets of 3-4 cms were potted in 1:1:1 mixture of sand, silt and peat moss under high humidity and further hardened under green house conditions. Using this system, we have been able to regenerate approximately 70% of healthy plantlets.
  Syed Sarfraz Hussain , Tayyab Husnain and S. Riazuddin
  Current approaches of cotton improvement include the use of genetic engineering, but progress in this area is limited because of notoriously recalcitrant nature of most elite cotton cultivars in tissue culture. A well-established regeneration system is desired for the improvement of cotton through genetic engineering. As it is reported that somatic embryos have been obtained from the regenerable lines of Coker 312 and Coker 315 but the problem with these varieties was the loss of embryogenic nature of callus with the passage of time. A procedure of recurrent somatic embryogenesis and twin embryo production in Gossypium hirsutum L Cv. Coker 312JS is being reported for the first time from the older somatic embryos. Calli were subcultured regularly. Embryogenic capacity was remained stable for more than two years. Similarly, twin embryo production was seen in second cycle of this system. This will help to propagate embryos for the development seed technology and gene transfer system.
  Syed Sarfraz Hussain , Tayyab Husnain and S. Riazuddin
  The study was undertaken to develop a culture technique permitting to grow cotton embryos from fertilization to germination with minimum of manipulation and maximum yield of viable plants. During the study, conditions were optimized for transfer of zygotic ovules after post anthesis and selection of media for the growth of ovules. Forty-eight hours post anthesis was selected as the most appropriate time since >50% of ovules were found alive, floating and developing. Also, ST medium was found suitable as it supported the growth very well and ovules continued to grow till the end of 10 weeks. This in-ovule technique has several advantages over culture of isolated embryos. The aseptic removal of 48 h old ovules from the ovaries was rapid and efficient and minimum damage was observed in this technique. No change of medium was needed during the entire culture period. This technique was used for the first time as a means of cotton transformation using Sonication assisted Agrobacterium mediated transformation (SAAT) method while a marker gene was used to transform cotton ovules.
  Tahira Fatima , Asad Jan , Tayyab Husnain and Sheikh Riazuddin
  Efforts were made to optimize tissue culture conditions for quick establishment of cell suspension and simple procedure for the cryopreservation of embryogenic cells suspension cultures of three rice varieties. Suspension cultures were initiated from friable, globular embryogenic calli in MS/R2 media supplemented with 2mg/l 2,4-D. These suspension cultures were regenerated and cryopreserved. Both MS and R2 media supplemented with 2mg/l 2,4-D were quiet suitable for developing cell suspension. All the three varieties gave compact light yellow calli on MMS medium. The regeneration frequency was 55% on average for 10-14 weeks old cell suspension. Vigorous regeneration was observed on MS containing sorbitol. Post thaw cell viability varied from variety to variety. No significant difference in viability was found in varieties cryopreserved for different periods. In conclusion cryopreservation can be used to preserve cell suspension line for a reasonable time. It can easily be used on culture medium or liquid R2 medium.
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