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Articles by Tapas Kumar Goswami
Total Records ( 2 ) for Tapas Kumar Goswami
  M. Yaqoob Wani , Tapas Kumar Goswami , Raies Ahmad Mir , Pallab Chaudhuri and Kuldeep Dhama
  Gram negative sepsis and septic shock are among the leading causes of death, both in humans and animals. Statins, 3-hydroxy-3-methylglutaryl coenzyme (HMG-CoA) reductase inhibitors are said to have immune modulating effects. In the present study, it was hypothesized that amelioration of hyper immune activation by pravastatin can improve the immunoapthological status of acute sepsis. Pasteurella multocida Pm52 strain was used as a source of Lipopolysaccharide (LPS) and the pathogenic organism for induction of septicaemia in mice. In vitro trials showed that LPS extracted from P. multocida stimulated Nitric Oxide (NO) production in time and dose dependent manner in Mouse Embryonic Fibroblast (MEF) cultures. Addition of pravastatin to MEF culture supernatant significantly reduced Pm52 LPS induced NO production (p<0.05). In vivo studies showed that administration of pravastatin in combination with cefotaxime to P. multocida induced septicaemic mice significantly increased both mean survival time and survivability percentage compared to antibiotic and pravastatin treatments regimes. Furthermore, the serum TNF-α : IL-10 levels were significantly improved and near to normal healthy ratios in septicaemic mice treated with pravastatin+cefotaxime combination at 24 h post infection. Gross and histopathological findings revealed moderate lesion in pravastatin treated mice as compared to untreated and cefotaxime alone treated groups. The findings conclude that pravastatin stabilizes the immune compromised status of the septicaemic animals during early septic stages by stabilising the NO production, regulating the TNF-α: IL-10 ratio and reducing histopathological lesions. Although the mortality was not prevented, the immunopathological signs were ameliorated to a greater extent by this new treatment combination, further investigations are suggested to explore its possible therapeutic utility against sepsis and for septicaemic patients.
  M. Yaqoob Wani , Tapas Kumar Goswami , Divya Chaudhary , Rajendra Singh and Kuldeep Dhama
  Lipopolysaccharide is the most important component of Gram negative bacteria that activates immune system through TLR-4 pathway. In the present study, the adjuvant effects of Pasturella multocida 52 strain (Pm52) on Hen Egg-white Lysozyme (HEL) and the possible attenuation of septic signs by pravastatin were investigated. In vitro results showed that Pm52 LPS stimulates nitric oxide (NO) production from splenocytes in time and dose dependent manner where as pravastatin (10 μM) inhibited the LPS induced NO production to basal levels (p<0.05). Furthermore, presence of pravastatin (10 μM) does not inhibit the in vitro proliferative responses of splenocytes by graded doses of Pm52 extracted LPS. The co-administration of Pm52 LPS with HEL by subcutaneous route in mice significantly increased TNF-α and NO levels at 2 and 6 h, respectively, as well as the HEL specific IgG development at 14th day Post Inoculation (PI). Administration of pravastatin by intraperitoneal route significantly reduced serum TNF-α and NO levels, the pro-inflammatory mediators responsible for induction of septic shock as well as stabilized TNF-α: IL-10 ratio without affecting HEL specific adaptive immune responses. However, further studies are required in this direction to determine the long term immune modulating effects of LPS and pravastatin on the host animals so as to be used in a beneficial way.
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