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Articles by Tahira Fatima
Total Records ( 3 ) for Tahira Fatima
  Asad Jan , Qazi M. Hassan , Tahira Fatima and Tayyab Hasnain
  Four rice genotypes i.e. Swat I, Swat II, Dilrosh 97 and Pakhal were tested for their callus induction frequency and their subsequent regeneration from different explants on a variety of media combinations. Swat I appeared to be the most responsive genotype to callus induction followed by Swat II, Dilrosh 97 and Pakhal respectively. Swat I and Swat II produced high amount of callus compared to other genotypes. Callus induction frequencies ranged from 68.88 to 57.70 percent in Swat I, 60.00 percent in Swat II, 52.20 percent in Dilrosh 97 and 40.00 to 42.20 percent in Pakhal from scutella on two different media. Similar response was also found for root induced calli. However, N6 medium containing 2 mg/l of 2,4-D was found to be optimum for callus induction irrespective of the genotypes and explant studied. All varieties showed significant differences in regeneration from two and three week old calli. RM medium containing MS salts and vitamins, 2 gm casamino acid, 1 mg/l NAA, 2 mg/l BAP and 30 gm sorbitol gave comparatively higher regeneration response than the other two media tested. Plants regenerated were grown in harmone free MS medium for vigorous rooting and subsequent transfer to soil.
  Sultana Rasheed , Tahira Fatima , Khurram Bashir , Tayyab Husnain and Shiekh Riazuddin
  Seeds of three indica rice varieties B-370, B-2000 and Super basmati were sterilized and incubated on callus inducing medium (MS-Medium with 2 mgl-1 2,4-D) for 2, 4, 6 and 8 weeks and calli were obtained after somatic embryogenesis. After the incubation periods, calli were transferred to the regeneration medium. Regeneration efficiencies decreased from 98.5, 98 and 95% after two weeks incubation to 30, 34 and 24% after eight weeks incubation for B-370, Super basmati and B-2000 respectively. Some interesting patterns for adventitious root formation were also observed. Significant variation was observed within and among different cultivars and incubation periods for average number of tillers, average plant height, number of panicles per plant, panicle length and average yield per plant. Seven interesting somaclones were marked and scored for agronomic and physiochemical analysis. Physiochemical studies did not reveal any significant variation. Present studies clearly indicated that somaclonal variation can successfully be used for creating genetic variation for varietal improvement.
  Tahira Fatima , Asad Jan , Tayyab Husnain and Sheikh Riazuddin
  Efforts were made to optimize tissue culture conditions for quick establishment of cell suspension and simple procedure for the cryopreservation of embryogenic cells suspension cultures of three rice varieties. Suspension cultures were initiated from friable, globular embryogenic calli in MS/R2 media supplemented with 2mg/l 2,4-D. These suspension cultures were regenerated and cryopreserved. Both MS and R2 media supplemented with 2mg/l 2,4-D were quiet suitable for developing cell suspension. All the three varieties gave compact light yellow calli on MMS medium. The regeneration frequency was 55% on average for 10-14 weeks old cell suspension. Vigorous regeneration was observed on MS containing sorbitol. Post thaw cell viability varied from variety to variety. No significant difference in viability was found in varieties cryopreserved for different periods. In conclusion cryopreservation can be used to preserve cell suspension line for a reasonable time. It can easily be used on culture medium or liquid R2 medium.
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