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Articles by T.V. Meenambigai
Total Records ( 4 ) for T.V. Meenambigai
  P. Hepsibha , T.V. Meenambigai , A. Mangalagowri , A. Palanisamy , A. Stalin , S. Nithya and K. Kumanan
  The present study has been undertaken for isolation, characterization and differentiation of Buffalo Adipose Derived Mesenchymal Stem Cells (bADMSCs). Cocktail enzymatic digestion method was used for isolation of bADMSCs which yielded optimum number of cells than other methods. Subcutaneous adipose tissue yielded comparatively higher number of Stem Cells (SC) than from omentum and bone regions. The growth and proliferation of SC were rapid when Mesencult medium was used, in comparison to Dulbecco’s Modified Eagles Medium (DMEM). Expression of oct 4 gene by reverse transcriptase-polymerase chain reaction (RT-PCR) indicated the stemness of isolated bADMSCs. Magnetic cell sorting and immunostaining revealed that the isolated ADMSCs were cluster of differentiation 44 (CD44) positive. Buffalo ADMSCs were also induced to differentiate into adipogenic, osteogenic and chondrogenic lineages. Expression of specific marker genes-Peroxisome Proliferator Activated Receptor Gamma (PPAR γ) in adipogenic, Osteopontin in osteogenic and Collagen II in chondrogenic differentiated lineages were confirmed in buffalo Adipose Derived Stem Cells (ADSCs) by RT-PCR. The study clearly proves that Buffalo white adipose tissue is a source of multipotent SC and can be induced to differentiate into adipogenic, osteogenic and chondrogenic lineages. Further, this is the first study to report such finding in buffalo.
  T.V. Meenambigai and V. Sejian
  Embryonic Stem (ES) cells are derived from blastocyst and these cells have the capability to generate all embryonic tissues in vitro. This propensity of ES cells has acquired considerable attention in recent years due to the promising potential for future cell replacement-based therapies. The in vitro differentiation capacity of ES cells provides unique opportunities for experimental analysis of gene regulation and function during cell commitment and differentiation in early embryogenesis. The ES cells are pluripotent cell lines with the capacity of self-renewal and a broad differentiation plasticity. They are derived from pre-implantation embryos and can be propagated as a homogeneous, uncommitted cell population for an almost unlimited period of time without losing their pluripotency and their stable karyotype. The ES cell technology is of high interest for researchers associated with livestock species. Simultaneously, research activities are being focused on characteristics and differentiation potential of Somatic Stem Cells (SSCs), unraveling an unexpected plasticity of these cell types. Somatic stem cells are found in differentiated tissues and can renew themselves in addition to generating the specialized cell types of the tissue from which they originate. Additional to discoveries of SSCs in tissues that were previously not thought to contain these kinds of cells, they also appear to be capable of developing into cell types of other tissues, but have a reduced differentiation potential as compared to embryo-derived stem cells. Therefore, SSCs are referred to as multipotent rather than pluripotent. This review summarizes characteristics of pluripotent ESCs in bovines and evaluates their potentials for in vitro propagation and differentiation as well as their potential uses in cell based therapies.
  V. Sejian , T.V. Meenambigai , M. Chandirasegaran and S.M.K. Naqvi
  Farm animal selection and reproduction are on the threshold of the application of new biotechnologies. Modern biotechnologies will allow advances to be made. Research into physiology and embryology has provided a basis for the development of technologies that increase productivity of farm animals through enhanced control of reproductive function. The livestock provides many opportunities to utilize these disciplines and evolving competencies. Artificial insemination, embryo transfer, in vitro fertilization, cloning, transgenics and genomics all are components of the tool box for present and future applications. Understanding the mechanisms that regulate reproductive function has important implications for this diverse field. Several peptides play a role in determining the normal functioning of the neuroendocrine regulation of reproduction. Kiss1 neurons have emerged as primary transducers of internal and environmental cues to regulate the neuroendocrine reproductive axis. Leptin serves as a metabolic signal that acts on the hypothalamic-pituitary-ovarian axis to enhance GnRH and LH secretion and ovarian function. Leptin effects on Gonadotropin-Releasing Hormone (GnRH) /LH secretion are mediated by NPY and kisspeptin. In recent years, livestock productivity has been increased by improved reproduction. Various techniques have been developed and refined to obtain a large number of offspring from genetically superior animals or obtain offspring from infertile animals. These techniques include: artificial insemination, cryopreservation of gametes or embryos, induction of multiple ovulations, embryo transfer, in vitro fertilization, sex determination of sperm or embryos, nuclear transfer, cloning, etc. Further the wide development radio-immuno-assay technology offers wide scope for improving the reproductive efficiency of farm animals. RIA technique for early non-pregnancy diagnosis can be integrated in to AI programmes in order to increase their effectiveness, reduce the unproductive period of dairy cows and increase the economic benefits to farmers. The greater challenge lies ahead for animal researchers is to integrate and potentially exploit these novel technologies in a society-friendly manner. Accepting this challenge and working towards achieving such targets should enable us to reap maximum benefits from the farm animal sector.
  Rajni Chhetri , T.V. Meenambigai and Veerasamy Sejian
  This review focused on our current understanding of the renal adult stem cells and their participation in kidney repair and regeneration. Currently, cells (growing in vitro) are being used as a replacement therapy/regenerative medicine with the great potential to treat kidney failure or other degenerative diseases. Regenerative medicine is now considered of great hope not only to control but also to cure some of the diseases which is otherwise difficult to treat. Recent studies have indicated that adult stem cells, either in the kidney itself or derived from the bone marrow, could participate in this repair process and might therefore be utilized clinically to treat acute renal failure. After renal ischemic injury, there is a upregulation of stromal cell-derived factor-1 expresson found in the kidney, which can induce leukocytosis and kidney repair. Renal stem cells, both from the renal papilla or the CD24+CD133+ cell niche of the Bowman’s capsule could differentiate into adult epithelial cells or tubular cells such as podocytes participate in this renal repair. Bone marrow-derived stem cells appeared to have a capacity for transdifferentiation and to be able to replace damaged renal tissue by replacing tubular epithelial cells, mesangial cells, endothelial cells and even podocytes. It is apparent from this review that there is a hidden potential within the kidney as well as in the bone marrow cells to stimulate endogenous or exogenous kidney regeneration. Further it can be speculated that harnessing the potential of these stem cells will go a long way in management and recovery of kidney failure through regenerative medicine approach.
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