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Articles by T.A. Sofi
Total Records ( 4 ) for T.A. Sofi
  B.A. Padder , M.D. Shah , Mushtaq Ahmad , T.A. Sofi , F.A. Ahanger and Aflaq Hamid
  Apple scab, caused by Venturia inaequalis, is one of the most damaging diseases worldwide on apple and is currently managed mainly by scheduled applications of fungicides. The aim of the present study was to understand the pathogen population structure in Jammu and Kashmir which is important for breeding and deployment of resistant cultivars. Twenty-seven isolates of V. inaequalis were sampled from commercial apple growing areas to estimate differences in pathogen populations using Random Amplified Polymorphic DNA (RAPD) 10 -and 20-mer primers. RAPD data analysis grouped 27 isolates in to three major clusters accommodating 10, 10 and 7 isolates each. The categories did not follow any geographic or source cultivar pattern. Allele frequencies among the three populations varied from 0.00 to 1.00. The average genetic diversity within each population (HS) over all loci studied was 0.21, 0.23 and 0.20 in Ganderbal, Pulwama and Srinagar, respectively thereby showing high genetic diversity within each population. The average genetic differentiation at a single locus among all sampled populations (GST) was 0.12. The overall gene flow (Nm) was 3.54 while the gene flow values at a single locus ranged from 0.70 to 69.32. Pair-wise genetic differentiation values (FST) among all loci were low, thereby indicating high diversity among the three populations. Analysis of molecular variance (AMOVA) obtained after clustering the isolates at district level showed highly significant genetic differentiation among populations with 5.46 and 94.54% genetic variability recorded among and within district populations, respectively. Population genetic analysis of V. inaequalis is the first molecular analysis of this pathogen from the India and especially from Jammu and Kashmir, a north-western Himalayan state of India.
  Lubna Masoodi , Ali Anwar , Shahzad Ahmed and T.A. Sofi
  Chilli is an important cash crop and India is the largest grower, consumer and exporter of dry chillies and other products to over 90 countries around the world. This crop suffers heavy losses in yield due to many diseases especially dieback and fruit rot diseases the frequent epiphytotics of the diseases in the Kashmir valley. During the past few years and extend of damage infelicated necessitated us to generate basic information on the important aspects like status, variability, hostrang and integrated management of the disease. Thus, the present study was undertaken to know the behavior of the disease and biology of the pathogen so as to device better managemental practices of the diseases to avoid losses. The objectives of the study were carried out as per the latest methodologies adopted by various workers in the world. Pathogenic behavior of twenty isolates of C. capsici, developed from fruits of chilli, was established following Koch’s Postulates. Colonies varied in their cultural behavior ranging from cottony to fluffy, mostly suppressed with regular to irregular margins. Colour of colonies ranged between white to grey. Growth rate of isolates was between 32.0-67.5 mm. Morphological studies of isolates revealed variations in their colour, size, shape, acervuli production, setae size and shape, conidia. Average conidial size varied from 2.23-33.6 μm and average setae size varied from 4.48-177.21 μm. On the basis of disease reaction expressed by differential hosts, ten groups (races) of C. capsici were identified. The group 1 comprised of isolates Cc-1, Cc-15 whereas group 2 included the isolate Cc-2, Cc-6, Cc-16. The Cc-3, Cc-10 were included in group-3 whereas group-4 included the isolate Cc-18, Cc-20, Cc-12, Cc-9. The group 5 comprised of isolates Cc-13, Cc-14. The group 6 comprised of Cc-17, Cc-19. The group 7 comprised of isolates Cc-5, Cc-11. Similarly, the isolate Cc-7 was clubbed under group 8.
  Farooq A. Ahanger , Gh. Hassan Dar , Muzafar A. Beig and T.A. Sofi
  Blue pine (Pinus wallichiana), the dominant species of pine widely grown in the Himalayan region including Jammu and Kashmir State, has been found affected by needle cast disease. Recently during forest survey for needle blight diseases conducted in the year 2010 a new fungal pathogen, Lophodermium macci Sokolski and Berube, was identified for the first time from Asia with the help of morphological characterization and ITS-1 DNA sequencing technique. A free hand section in lactic acid fuchsin was used for morphological characterization. For molecular identification DNA was isolated from hysterothecia formed on the infected needles. The fungus, previously recorded on Pinus strobus, has been noticed for the first time on Blue pine (Pinus wallichiana) and Aleppo pine (P. halepensis). The pathogen L. macci is closely (98%) related to L. pini-excelsae and L. nitens. The conidiomata of L. macci which have not previously been documented were observed for the first time. The conidiomata were subepidermal dark brown to black in color. Black zone lines were only present when adjacent to other Lophodermium species. Since Lophodermium macci Sokolski and Berube has not so far been reported from any of the Asian countries so this forms the first report of this fungus from Asia. Further, the presence of L. macci on Pinus wallichiana and P. halepensis are new host records worldwide.
  M.S. Dar , Irtefa Mohammed , T.A. Sofi , F.A. Ahanger , M.D. Shah , Mushtaq Ahmad , Aflaq Hamid , A.A. Mir , Asha Nabi and B.A. Padder
  Apple scab caused by a fungus Venturia inaequalis cause enormous losses to growers both qualitatively and quantitatively. In order to manage the crop, farmers routinely spray 8-12 fungicides from pink bud till harvest. Hence, the best strategy to manage the disease is planting apple scab resistant cultivars. In the present study, four races viz., (0), (1), (2) and (1, 2) were reported from various commercial apple growing regions of Kashmir. Apple scab races were spatially distributed in all apple growing regions of Kashmir. Race 1 was most prevalent among the four races. Screening of 31 apple genotypes under controlled conditions revealed majority of commercial cultivars susceptible. Genotypes carrying RVi3 to RVi13 scab resistance genes along with the cultivars American Apirouge, H27, Shireen and Firdous (latter two contain RVi6 scab resistance gene) were found resistant to all the four races present in Kashmir. Resistant genotypes reported in the present study can easily be exploited by breeders for management of apple scab resistance using marker assisted selection approach.
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