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Articles by T. Ren
Total Records ( 4 ) for T. Ren
  The objective of the present study was to investigate the effect of dietary phospholipid (PL) level on growth and feed intake of juvenile amberjack (Seriola dumerili) fed non-fishmeal (non-FM) diet containing alternative protein sources; soybean protein isolate, tuna muscle by-product powder and krill meal. Three non-FM diets were prepared to contain three levels (14, 37 and 54gkg−1 dry diet) of PL (soybean lecithin acetone insoluble, 886gkg−1) and growth performance was monitored in a 30-day growth trial by using 2.6g of fish. The results indicated that final body weight, weight gain and feed intake significantly increased with increasing dietary PL level. At the highest dietary PL level (54gkg−1 dry diet), the fish consumed 14.8% and 10.2% as much feed as those fish fed diets containing 14gkg−1 dry diet and 37gkg−1 dry diet PL, respectively. An increasing tendency with increasing dietary PL level on feed efficiency was observed. In conclusion, the present study demonstrated that dietary PL supplementation could increase feed intake, and improve the growth of juvenile S. dumerili fed non-FM diets. Therefore, purified PL might be a good candidate to stimulate the growth of fish through enhancing the feed intake when they are fed diets containing alternative protein sources.
  This study was conducted to examine the effects of dietary ascorbic acid (AsA) and phospholipid (PL) and their interaction on growth, survival, and stress resistance in red sea bream larvae. Twenty-six days old red sea bream were fed nine micro-bound diets supplemented three levels of AsA (0, 800 and 1600 mg kg−1 diet) and PL (0, 20 and 40 g kg−1 diet) for 15 days. Dietary AsA and PL were both significant factors on survival rates. There was also an interaction between dietary AsA and PL on survival rate (P < 0.05). The larvae fed 800 or 1600 mg kg−1 AsA with 40 g kg−1 PL diets showed the highest survival rate, with values similar to those of the live-food supplemented group. Stress resistance against low salinity exposure significantly increased with increased dietary level of AsA and PL. However, significant interaction of AsA and PL was not detected. The larvae fed 1600 mg kg−1 AsA with 40 g kg−1 PL diet showed the highest stress resistance among all diets, but it was not significantly different than that of larvae fed 800 mg kg−1 AsA with 40 g kg−1 PL diet. This study clearly demonstrated that combined use of AsA and PL can improve survival of 26–40 days posthatching red sea bream larvae. Moreover, the present study suggested that 800 mg kg−1 AsA with 40 g kg−1 PL in diet was needed for producing high quality seedling under the stressful conditions.
  Shi-Min Gao , Min-Hua Sun , Zhao-Xiong Wang , Xian-Wei Li , Hui-Ying Fan and T. Ren
  Newcastle Disease (ND) is caused by Newcastle Disease Virus (NDV) with a respiratory and digestive mucosal bleeding for typical symptoms. It is an acute highly contacting disease. NDV could multiplicate in DF-1 cells. NDV from waterfowl is different from former NDV from chicken, it lead waterfowl dead. But the molecular mechanisms of waterfowl NDV infected still are poorly understood. In this study researchers employed the illumina genome analyzer platform to perform genome-wide Digital Gene Expression (DGE), a tagbased high-throughput transcriptome sequencing method analysis of DF-1 cells infected NDV-YC. Researchers could reach a sequencing depth of 5.0-6.1 million tags per library and found >8000 genes to be differentially expressed during NDV-YC infection processes. NDV-YC notably identified genes involved in I-kappaB kinase/NF-kappaB and TGF-beta signaling pathway. Further analysis employed Gene Ontology (GO) analysis cell part, binding and biological regulation terms are main items, respectively. The data benefit for better understanding the molecular mechanism of NDV-YC infected and provide a basis for further in vivo study and clinical trials.
  J. L. Heitman , R. Horton , T. Ren , I. N. Nassar and D. D. Davis
  Diffusion-based coupled soil heat and water transfer theory includes capability to describe transient behavior. Unfortunately, laboratory tests of theory typically include a single initial water content distribution with a single set of boundary conditions, rather than providing a set of experimental conditions with a range of measurements for comparison with predictions. Agreement between theory and measurements can result from calibration, but this provides an incomplete test of theory. The objective of this work was to test diffusion-based coupled heat and water transfer theory by comparing theory-based predictions with measured transient temperature and water content distributions. Data from a single boundary condition were used for calibration of each of two soils, silt loam and sand. Subsequent testing was performed at additional boundary and initial conditions using measurements from the same soil. Results indicate that the theory can be calibrated for a single boundary condition with adjustment of soil saturated hydraulic conductivity and/or the vapor enhancement factor, which adjust the liquid and vapor fluxes, respectively. For silt loam, calibration reduced Root Mean Square Error (RMSE) by 67 and 18% for water content and temperature distributions, respectively. For sand, RMSE was reduced by 14 and 46% for water content and temperature, respectively. Using this calibration, there was agreement between calculated and measured distributions for additional boundary and initial conditions with RMSE ≤ 0.03 m3m–3 and 1.28°C for water content and temperature distributions, respectively. However, when the boundary temperature gradient was instantly reversed, noticeable differences occurred between measured and calculated patterns of heat and moisture redistribution. The theory described observations well when boundary temperature conditions were changed gradually, but results suggested a need for further development of coupled heat and water transfer theory combined with testing under transient conditions to make improvements in the description of transfer mechanisms.
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