|
|
| |
| Articles
by
T. Behera |
Total Records (
6 ) for
T. Behera |
|
 |
| |
|
| |
P.K. Nanda
,
P. Swain
,
S.K. Nayak
,
T. Behera
and
K. Dhama
|
| |
Enzymatic method using trypsin to establish primary culture from various cells
from heart, liver, kidney, brain, testis, ovary, fin and embryo of Indian Major
Carp, Cirrhinus mrigala was investigated and compared with that of explant
culture method. The dissociation time to obtain cells from individual tissues
and the viability of cells for primary culture varied at 0.125 and 0.25% trypsin
concentration. Single cell culture of trypsinised cells from testis, ovary and
embryo showed good attachment irrespective of trypsin concentration. Cells from
liver and heart were found to be sensitive to trypsin only at higher concentration
(0.25%); whereas, no attachment/or proliferation was observed from cells of
kidney, brain and fin due to sensitivity to trypsin at both the concentrations.
In contrast to this method to obtain primary culture, explant culture from most
of the tissues showed better attachment, resulting in subsequent growth and
proliferation of cells forming monolayer. Overall, the explant culture of most
of the tissues of C. mrigala was found to be suitable and survived more
passages as compared to single cell culture obtained through trypsinisation.
For obtaining primary culture from single cells, further investigations are
desired to identify tissue specific enzymes, standardization of dose, duration
and temperature of enzymatic treatment. |
|
| |
|
| |
P.K. Nanda
,
P. Swain
,
S.K. Nayak
,
T. Behera
,
P. Jayasankar
and
K. Dhama
|
| |
The success in establishing in vitro culture from anchorage dependent tissue explants depends upon their ability to attach to culture substratum. In this study, evaluation of different coating factors on the attachment and subsequent monolayer formation from various tissue explants of Indian Major Carp, Mrigal, Cirrhinus mrigala was investigated to establish cell culture. Explants from heart, liver, gills, testis, ovary, kidney and fin were taken in respective culture flasks coated either with type I collagen, gelatin or fibronectin to know their ability on attachment and proliferation of cells resulting in attaining confluency. The percentage attachment of explants from same tissue, when cultured in different coated flasks, varied greatly. Explants (40-65%) from liver, ovary, testis and fin were found to attach well in fibronectin coated flasks, whereas maximum attachment (>65%) from heart tissue was recorded in gelatin coated flasks. This difference in percentage attachment of explants to substratum significantly affected growth, proliferation of cells and subsequent formation of confluent monolayer. Overall, fibronectin as a coating factor was found to be good for attachment of explants from most of the tissues of C. mrigala. Contrary to this, attachment percentage of explants from fin, gills, ovary and testis in type I collagen coated flasks was less which eventually failed to form monolayer. The findings indicate that coating factor(s) has a major role in establishing cell culture which not only influences attachment but also growth and proliferation of cells from explants obtained from different tissues of C. mrigala. Further investigations are suggested to find out suitable coating factors for each cell types of different fish species so as to establish cell cultures. |
|
| |
|
| |
P. Mohapatra
,
R.K. Swain
,
S.K. Mishra
,
T. Behera
,
P. Swain
,
N.C. Behura
,
G. Sahoo
,
K. Sethy
,
B.P. Bhol
and
K. Dhama
|
| |
To study the comparative effects of Nano Selenium (Nano-Se) and sodium selenite
on the growth, bioavailability, antioxidative activities, hematological and
biochemical parameters, cellular and humoral immunity, a trial was conducted
on BV 300 layer birds during grower phase (9-20 after weeks) in six treated
groups. After twenth weeks body weight of the all Nano-Se treated groups (up
to a dose of 0.3 mg kg-1 of diet) was found to be significantly (p<0.05)
higher than sodium selenite treated and control groups. However, further increase
in dietary Nano-Se content in feed had negative effect on body weight of bird.
Birds fed with both Nano-Se and sodium selenite showed higher (p<0.05) Se
content in different tissues (Such as breast muscle, liver, kidney, pancreas,
serum and feathers). However, Se content in liver, breast muscle, pancrease
and feathers were signifcantly higher (p<0.05) in Nano-Se treated groups.
In addition, significant (p<0.05) difference was observed as regard to glutathione
peroxidase (GSH-Px), erythrocyte catalase (CAT) and superoxide dismutase (SOD)
activities among the treated groups. Significantly better cellular and humoral
immunity response were observed in Se supplemented birds. Dietary supplementation
of Nano-Se improved the body weight, feed consumption ratio, antioxidant status,
immunity and tissue Se deposition in grower birds. |
|
|
| |
|
| |
P. Mohapatra
,
R.K. Swain
,
S.K. Mishra
,
T. Behera
,
P. Swain
,
S.S. Mishra
,
N.C. Behura
,
S.C. Sabat
,
K. Sethy
,
K. Dhama
and
P. Jayasankar
|
| |
Comparative study on the effect of nano selenium (nano-Se) and sodium selenite on the growth, bioavailability, antioxidative activities, hematological and biochemical parameters, cellular and humoral immunity was done in layer chicks upto 8th week post feeding. The results showed significant differences (p<0.05) in relative weight gain and final body weight of the nano-Se treated groups upto a dose of 0.3 mg kg-1 of diet as compared to sodium selenite and control groups. However, further increase in dietary nano-Se content in feed had negative effect on weight and Relative Gain Rate (RGR). Survival rate and Feed Conversion Ratio (FCR) were not affected by dietary treatments. Chicks fed with both nano-Se and sodium selenite showed higher (p<0.05) Se content in different tissues (breast muscle, liver, kidney, pancreas, serum and feathers). However, highest value (p<0.05) of Se content in breast muscle and liver was observed in nano-Se treated groups. Selenium concentrations in serum, liver and breast muscle increased linearly and quadratically (p<0.05) as dietary Se level increased for all Se sources but its magnitude was substantially greater (p<0.05) when nano-Se was fed. Glutathione peroxidase (GSH-Px), erythrocyte catalase (CAT) and superoxide dismutase (SOD) activities were significantly different (p<0.05) in all treated groups than control. Dietary nano-Se also increased several serum biochemical and haematological parameters. In addition, it significantly increased both cellular and humoral immunity in layer chicks after 8th weeks of post feeding. In conclusion, dietary administration of nano-Se was found superior than that of inorganic sodium selenite in various aspects in layer chicks. Further extensive study for exploring absorption mechanisms, metabolic pathways, ideal dose/form of nano-Se is suggested for optimum utilization of nano-material based application of Se feeding in poultry. |
|
| |
|
| |
A. Das
,
S.K. Mishra
,
R.K. Swain
,
G. Sahoo
,
N.C. Behura
,
K. Sethi
,
B. Chichilichi
,
S.R. Mishra
,
T. Behera
,
K. Dhama
and
P. Swain
|
| |
A comparative study on effect of replacement of inorganic minerals viz., Zinc (Zn), Copper (Cu) and Manganese (Mn) with their corresponding organic minerals (methionine) on growth, bioavailability and immunity in layer chicks was undertaken till 8th week post feeding. At 8th week, the body weight of birds were found to be significantly (p<0.05) higher in 100% organic Zn group, 100 and 50% organic Zn, Cu and Mn supplemented groups. The cumulative feed consumption and Feed Conversion Ratio (FCR) of all the treated groups showed no significant (p>0.05) difference. The serum glucose, cholesterol and ALP levels showed significant (p<0.05) differences. Significantly (p<0.05) higher titer levels were observed in 100% organic Zn group, 100% and 50% organic Zn, Cu and Mn supplementation groups. The CBH response of all the treated groups showed no significant (p>0.05) difference. At 8 weeks of age, the relative weight of spleen of layer chicks in 100% and 50% organic Zn, Cu and Mn supplementation groups were significantly (p<0.05) higher than all the other treatments. Tibia bone weight (g), tibia calcium (%) and tibia phosphorus (%) varied insignificantly (p>0.05). But significant (p<0.05) difference was observed with respect to the tibia ash content. Except Zn and Mn levels of tibia, all other studied mineral levels of serum and liver did not differ significantly (p>0.05). Faecal excretions of minerals were significantly lower in organic mineral fed groups. Replacement of inorganic Zn, Cu and Mn with corresponding organic minerals improved the body weight, immunity and lower faecal excretion of minerals in chicks. |
|
| |
|
| |
Abinash Das
,
S.K. Mishra
,
R.K. Swain
,
P. Swain
,
K. Dhama
,
G. Sahoo
,
N.C. Behura
,
K. Sethy
,
B. Chichilichi
,
T. Behera
and
S.R. Mishra
|
| |
Effect of replacement of inorganic minerals viz., zinc (Zn),
copper (Cu) and manganese (Mn) with their corresponding organic minerals (methionine)
on growth, bioavailability and immunity was studied in grower birds (9th to
20th week). At 20th week, the body weight of grower birds were significantly
(p<0.05) higher in 100% organic Zn group, 100 and 50% organic Zn, Cu and
Mn supplemented groups. The cumulative feed consumption and FCR of all the treated
groups showed no significant (p>0.05) difference. The serum glucose, after
cholesterol SGOT, SGPT and ALP levels differ significantly (p<0.05) of all
the treated groups of differed significantly (p<0.05). The CBH response and
the antibody titers against SRBC were found to be significantly higher in 100%
organic Zn group and 100% Zn, Cu and Mn supplemented groups. Tibia bone weight
(g), tibia calcium (%) and tibia phosphorus (%) varied insignificantly (p>0.05).
But significant (p<0.05) difference was observed as regard to the tibia ash
content. Faecal excretions of Zn, Cu and Mn were significantly lower in organic
mineral fed groups. Replacement of inorganic Zn, Cu and Mn with their corresponding
organic minerals improved the body weight and immunity, with lower faecal excretion
of minerals in grower birds. |
|
|
|
|
| |
|
