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Articles by Syed Sarfraz Hussain
Total Records ( 5 ) for Syed Sarfraz Hussain
  Syed Sarfraz Hussain , Faqir Muhammad Azhar and Ishtiaq Mahmood
  The correlation studies showed that in the plant material studied, plant height, monopodial branches and No. of bolls per plant were revealed to be significantly correlated with seed cotton yield at genotypic level. The correlation between No. of bolls per plant and plant yield appeared to be strong as compared with other combinations. In addition, plant height showed positive and significant relationship with No. of sympodial branches, No. of bolls per plant and ginning %age. Path coefficient analysis revealed that No. of monopodial branches, No. of bolls per plant had maximum positive direct effect on yield of seed cotton. Therefore, it may be suggested that negative direct effects of plant height, No. of sympodial branches and ginning out turn may be considered as selection criteria to effect indirect improvement in yield of seed cotton and quality in the present breeding material.
  Rozina M. Ali , Tayyab Husnain , Syed Sarfraz Hussain , Nasir Mahmood and Sheikh Riazuddin
  Induction of multiple shoots in Gossypium hirsutum L. variety CIM-443 has been achieved by using meristem and cotyledonary node as explants. Meristems of size 3-4 mm were excised from embryos isolated from seeds while cotyledonary nodes were cut from seven days old seedling and the mean shoot number per explant response for total of three experiments was maximum i.e., (6.72±0.79) and (4.92±0.67), respectively for both explants in MS medium supplemented with benzyl-amino purine (BAP) 1.0 mg L-1 + naphthalene acetic acid (NAA) 0.05 mg L-1. Shoot elongation was observed in MS medium amended with NAA 0.1 mg L-1. The percentage of shoots forming roots was maximum (79.16) in case of 1/2 MS supplemented with NAA 0.05 mg L-1. Rooted plantlets hardened in soil and normal boll formation observed.
  Syed Sarfraz Hussain , Tayyab Husnain and S. Riazuddin
  Successful cotton plant transformation depends on regeneration of plants from transformed cells. Recalcitrance of cotton to tissue culture has not only slowed the development of transgenic cotton but also narrowed its genetic base. Keeping this in view, an efficient in vitro plant regeneration system characterized by bulk, rapid and continuous production of somatic embryos using immature zygotic embryos, taken at various stages of development from ovules as explant has been developed in cotton. One of the drawbacks of using this is the requirement of large number of high quality immature zygotic embryos. To address this problem, we have developed a procedure that generates highly homogeneous populations of embryogenic calli by selectively propagating a small number of regeneration proficient calli derived from immature zygotic embryos. Stages showing the early cotyledon development cultured on modified Murashige and Skoog media proliferated intensely. Rapid callogenesis was observed from these immature zygotic embryos. To induce germination and plantlet growth, embryoids were placed on sterile processed cotton, saturated with Stewart and Hsu media. Upon development of roots and leaves, plantlets of 3-4 cms were potted in 1:1:1 mixture of sand, silt and peat moss under high humidity and further hardened under green house conditions. Using this system, we have been able to regenerate approximately 70% of healthy plantlets.
  Syed Sarfraz Hussain , Tayyab Husnain and S. Riazuddin
  Current approaches of cotton improvement include the use of genetic engineering, but progress in this area is limited because of notoriously recalcitrant nature of most elite cotton cultivars in tissue culture. A well-established regeneration system is desired for the improvement of cotton through genetic engineering. As it is reported that somatic embryos have been obtained from the regenerable lines of Coker 312 and Coker 315 but the problem with these varieties was the loss of embryogenic nature of callus with the passage of time. A procedure of recurrent somatic embryogenesis and twin embryo production in Gossypium hirsutum L Cv. Coker 312JS is being reported for the first time from the older somatic embryos. Calli were subcultured regularly. Embryogenic capacity was remained stable for more than two years. Similarly, twin embryo production was seen in second cycle of this system. This will help to propagate embryos for the development seed technology and gene transfer system.
  Syed Sarfraz Hussain , Tayyab Husnain and S. Riazuddin
  The study was undertaken to develop a culture technique permitting to grow cotton embryos from fertilization to germination with minimum of manipulation and maximum yield of viable plants. During the study, conditions were optimized for transfer of zygotic ovules after post anthesis and selection of media for the growth of ovules. Forty-eight hours post anthesis was selected as the most appropriate time since >50% of ovules were found alive, floating and developing. Also, ST medium was found suitable as it supported the growth very well and ovules continued to grow till the end of 10 weeks. This in-ovule technique has several advantages over culture of isolated embryos. The aseptic removal of 48 h old ovules from the ovaries was rapid and efficient and minimum damage was observed in this technique. No change of medium was needed during the entire culture period. This technique was used for the first time as a means of cotton transformation using Sonication assisted Agrobacterium mediated transformation (SAAT) method while a marker gene was used to transform cotton ovules.
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