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Articles by Sweda Sreekumar
Total Records ( 2 ) for Sweda Sreekumar
  Elayarajah , R. Rajendran , Venkatrajah , Sweda Sreekumar , Asa Sudhakar , Janiga and Soumya Sreekumar
  Biomaterial-centred bacterial infections present common and challenging complications with medical implants like ureteral stent which provide substratum for the biofilm formation. Hence the purpose of this study is to make antibacterial stent surface with biodegradable polymer (tocopherol acetate) and anti-infective agents (norfloxacin and metronidazole) using a modified dip-coating procedure. This is done by impregnating the stent pieces in the anti-infective solution (a mixture of norfloxacin-metronidazole and polymer) for uniform surface coating (drug-carrier-coated stents). After coating, agar diffusion test was performed as qualitative test to find out the sensitivity of coated stents against the clinical isolates, Staphylococcus epidermidis and Escherichia coli. Quantitative test was measured by calculating the numbers of adhered bacteria on coated and uncoated stents by incubating the stent pieces in artificial urine. Difference in the number of viable bacteria adhered on the surface of coated and uncoated stents were statistically calculated using chi square test with p<0.05 considered significant. The stent colonising ability of Staphylococcus epidermidis and Escherichia coli in a controlled environment chamber was determined using two-challenge dose of the isolates by in vitro challenge test. In qualitative test, the zone of inhibition around the coated stents showed sensitivity against the clinical isolates. In quantitative test, the number of adhered bacteria on the surface of coated stents was reduced to a significant level (p<0.05). The polymer, tocopherol acetate is highly biodegradable in nature. Due to its degrading ability in body tissues, it releases the anti-infective drugs at a constant and sustained rate.
  Sweda Sreekumar , B. Elayarajah , R. Rajendran , B. Venkatrajah , Soumya Sreekumar and Selin Jacob
  Coronary artery disease usually requires angioplasty-involving stents that help in keeping the artery open for proper blood flow. Such stents may present surfaces for colonization of biofilm forming bacteria thereby causing stent-associated infections. The purpose of this study was to provide anti-infective Poly Tetra Fluoro Ethylene (PTFE) stents for the prevention of biofilm formation. Anti-infective mixture of synergistic drugs with biodegradable carrier, DL-lactic Acid (DLLA) was used to coat PTFE stents by means of dip-coating procedure. FTIR analysis of PTFE stent was used to determine the presence of bound anti-infective drugs. Antibacterial activity of anti-infective PTFE stents was determined qualitatively and quantitatively. In vitro challenge test was monitored to determine the persistence of drugs on anti-infective PTFE stent surface. The Fourier Transform Infrared (FTIR) analysis showed two major peaks at 3101.64 and 1141.94 nm for the O-H stretching and .C=O stretching of COOH. Antibacterial activity was analysed by qualitative (Agar diffusion test) and quantitative (Bacterial adherence test) methods. The former showed largest inhibition zone for Pseudomonas aeruginosa (29 mm) and the latter confirmed that the number of adhered bacteria in drug-carrier coated stents (p<0.05) were less than the number of adhered bacteria in carrier-coated stents (p>0.05). In vitro challenge test clearly demonstrated that bacterial growth failed to develop even after three consecutive challenge doses. These drug-eluting stents could be of great interest for coronary stenting to prevent stent-associated infections if these results are confirmed in vivo.
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