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Articles by Sudhakar Indulkar
Total Records ( 1 ) for Sudhakar Indulkar
  Felix Sanudi , Sudhakar Indulkar and Kumbukani Mzengereza
  Background and Objective: Herbicides are chemicals which are employed to kill or control vegetation. Herbicides being used in agriculture industry have agents with the ability to cause death to plants and animals. Sub-lethal toxicity bioassay experiment was conducted to determine the toxicity of glyphosate herbicide on Koi carp, Cyprinus carpio fingerlings. Materials and Methods: Koi carp fingerlings with mean length 8.06±0.99 cm exposed to sub-lethal concentrations i.e., 1/10th (3.6 mg L1) and 1/5th (6.6 mg L1) of glyphosate. Blood samples were collected from both control and experimental group fishes by puncturing posterior caudal vein using a disposable 1.0 mL tuberculin syringe having needle size 0.45×13 mm for analysis at 7B day interval for a period of 28 days. Total white blood and red blood cells counts were carried out using Neubauer Haemocytometer. Haemoglobin percentage was determined following the Cyanmethemoglobin method. The micro-haematocrit method was used to determine the haematocrit. The derived haematological indices of mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH) and mean corpuscular haemoglobin concentration (MCHC) were calculated using the following standard formulae. Results: Findings revealed significant decrease (p<0.05) in red blood cells (1.06±0.008 to 0.60±0.029), haemoglobin (11.2±0.116 to 7.45±0.029), haematocrit (18.2±0.664 to 14.7±0.404), mean corpuscular haemoglobin concentration (62.7±1.628 to 45.8±1.199) and increase in white blood cells count (204.0±3 to 216.5±0.404), mean corpuscular volume (170.4±7.707 to 255.2±18.65), mean corpuscular haemoglobin (106.2±7.707 to 116.8±6.500). Conclusion: This indicated that glyphosate has impact on haematological parameters of exposed fishes. Thus, it is harmful to target, non-targeted species and the environment.
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