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Articles by Sri Suharti
Total Records ( 5 ) for Sri Suharti
  Anuraga Jayanegara , Mohammad M. Sholikin , Della A.N. Sabila , Sri Suharti and Dewi Apri Astuti
  Background and Objective: Cricket contains high crude protein level but it also contains considerable amount of chitin that may impede nutrient digestion and decrease production performance of animal. This experiment aimed to decrease chitin content of cricket (C) through exoskeleton removal (CER) or by chemical extraction (CCE). Materials and Methods: Nutritional evaluation of cricket was performed in two experiments. In experiment 1, three forms of cricket were prepared, i.e., C, CER and CCE. These were subjected to chemical composition determination and in vitro rumen fermentation incubation as individual substrates. In experiment 2, C and CER were included in concentrate rations at different proportions to substitute soybean meal (SBM), i.e., R1 (concentrate containing 30% SBM), R2 (50% SBM was substituted by C), R3 (100% SBM was replaced by C) and R4 (100% SBM was replaced by CER). The concentrates were then evaluated in vitro for their rumen fermentation and digestibility characteristics. Data were analyzed with analysis of variance and Duncan’s test. Results: Cricket was high in crude protein(CP), ether extract (EE) and chitin contents. Removal of exoskeleton decreased CP and chitin contents of cricket. Chemical extraction of cricket increased its CP and completely removed its chitin. Main fatty acids observed in cricket were linoleic acid, palmitic acid, oleic acid and stearic acid and the composition was unaltered due to exoskeleton removal or chemical extraction. Cricket was relatively highly digestible and exoskeleton removal and chemical extraction did not further improve in vitro dry matter digestibility (IVDMD) and in vitro organic matter digestibility (IVOMD) of cricket. The R1 and R2 revealed similar IVDMD and IVOMD, but R3 and R4 resulted in lower values for both parameters than those of R1 and R2 (p<0.05). Conclusion: Exoskeleton removal or chemical extraction effectively reduced chitin content of cricket and the insect may be used to substitute SBM up to 50% in concentrate for ruminant.
  Sri Suharti , Dewi Apri Astuti , Elizabeth Wina and Toto Toharmat
  This research was aimed to investigate the utilization of whole lerak extract to improve rumen fermentation, nitrogen retention and performance of beef cattle received high forage based ration. Experimental diet composed of forage (70%) and concentrate (30%). The in vivo study was conducted using 12 local beef cattle which were divided into three treatments ie three different levels of lerak extract (0, 100 and 200 mg/kg body weight) were added to the diet. Parameters measured were nutrient digestibility, volatile fatty acid (VFA) profile, NH3 concentration, microbial protein synthesis, feed intake and daily gain of beef cattle during 90 days of feeding trial. The addition of lerak extract up to the level of 200 mg/kg BW did not affect nutrient digestibility. Total VFA and propionate proportion increased (p<0.05) and the ratio of acetate: propionate decreased (p<0.05) with the addition of lerak extract. Concentration of NH3 in the rumen tended to decrease. Nitrogen retention, microbial protein synthesis, feed intake and daily gain of local beef cattle fed high forage ration tended to increase with the addition of lerak extract at the level up to 200 mg/kg BW. The addition of lerak extract at the level of 200 mg/kg BW increased average daily gain up to 12.5% compared to the control treatment.
  Sri Suharti , Lilis Khotijah , Afdola Riski Nasution , Dewi Ayu Warmadewi , I. Gusti Lanang Oka Cakra , Chairussyuhur Arman and Komang G Wiryawan
  Objective: This study was aimed to evaluate the effect of calcium soap-soybean oil on productive and reproductive performances as well as blood profile of Bali cattle. Materials and Methods: This study used 12 heads of Bali cattle with the average body weight of 230 kg at Sobangan Breeding Centre, Bali Province, Indonesia. There were 2 dietary treatments i.e., control (no supplementation of calcium soap-soybean oil) and control+calcium soap-soybean oil 5%. Control diet which consist of Napier grass and concentrate with ratio approximately 80:20 and all treatment diets were offered for 60 days. All cows were oestrous synchronised using PGF2α injections and continued with artificial insemination. Variable measured were feed intake, body weight gain, average daily gain, feed efficiency, service per conception, blood nutrient and haematology. Results: The results showed that the addition of calcium soap-soybean oil significantly increased (p<0.05) feed consumption of forage and concentrate, but slightly decreased body weight gain and feed efficiency compared to the control treatment. Moreover, the addition of calcium soap-soybean oil significantly (p<0.05) enhanced reproductive performance of Bali cows such as pregnancy rate and proportion of cattle with service per conception (S/C) = 1. However, the use of calcium soap-soybean oil did not affect blood metabolites and blood haematology except white blood cells and leucocyte differentiation such as neutrophil, lymphocytes and monocyte. Conclusion: The use of calcium soap-soybean oil has positive effect on reproductive performance without altering the health status of Bali cows.
  Wulansih D. Astuti , Komang G. Wiryawan , Elizabeth Wina , Yantyati Widyastuti , Sri Suharti and Roni Ridwan
  Background and Objective: Probiotics are widely used in ruminant production, but information about the potential of Lactobacillus plantarum (L. plantarum) as a probiotic for ruminants is still limited. The aim of this research was to select L. plantarum strains as a probiotic for ruminants and to determine their effect on the rumen fermentation system. Materials and Methods: The first experiment was conducted using a randomized block design to select 14 strains of L. plantarum isolated from rumen cattle. The second experiment was arranged in a completely randomized design using two selected L. plantarum strains to determine their effects as a probiotic on rumen fermentation. The substrates used for in vitro fermentation were napier grass (Pennisetum purpureum) and concentrate in a 70:30 ratio. Results: From experiment 1, L. plantarum U32 was selected, because it produced low methane/total gas (27.39%) and strain U40 was selected because it had the highest dry matter and organic matter rumen disappearance (56.45 and 56.44%). In experiment 2, the addition of L. plantarum U32 and U40 as probiotics increased propionic acid and decreased acetic production (p<0.05), which led to a lower A:P ratio (p<0.05). The total volatile fatty acid and in vitro digestibility were not affected by the addition of L. plantarum. Probiotic addition increased lactic acid bacteria and the protozoa population (p<0.05) from the rumen fluid compared to the control. The total rumen bacteria were not significantly changed by the treatments. Conclusion: The addition of L. plantarum strains U32 and U40 as probiotics had beneficial effects for rumen fermentation due to increased propionic acid and decreased methane production.
  Sri Suharti , Fransiscus Xaverius Shila Kurnia , Bagus Pambudi and Komang G. Wiryawan
  Background and Objective: Leucaena (Leucaena leucocephala) is one of the potential legumes that is a source of protein feed for ruminants. Leucaena has approximately 24% protein. Leucaena usage is restricted because it contains the anti-nutrient mimosine (7.19%). Mimosine causes hair loss in sheep and inhibits the action of the hormone thyroxine. The purpose of this study was to evaluate the concentration of mimosine in the blood, rumen, urine, faeces, blood metabolites and thyroid hormones of sheep fed rations containing different levels of leucaena leaf meal. Methodology: There were two kinds of treatments: P1 = Napier grass 60+15% Gliricidia sepium+15% Leucaena leucocephala+10% Pollard and P2 = Napier grass 60+30% Leucaena leucocephala+10% Pollard. This experiment used a randomized block design. The variables observed in this study were the concentration of mimosine in the blood, rumen, urine, faeces, blood metabolites and thyroid hormones. Results: The results showed that different levels of leucaena leaf meal did not have significant effects on the mimosine concentration in the rumen, blood, urine, faeces, blood metabolites and thyroid hormones. Mimosine disappearance from consumption to excretion was between 34-68%. Conclusion: The addition of 30% Leucaena leucocephala leaf meal could be used without a negative effect on blood metabolites and thyroid hormones of sheep.
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