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Articles by Sikander Ali
Total Records ( 6 ) for Sikander Ali
  Umar Farooq Awan , Kiran Shafiq , Shazia Mirza , Sikander Ali , Asad-ur- Rehman and Ikram-ul- Haq
  Solid-state fermentation for lipase production was carried by Rhizopus oligosporous ISUUV-16. Almond meal was used as basal substrate with distilled water as moistening agent at substrate to diluent ratio of 1:0.7. Fermentation was carried out at 30oC and culture was incubated for 48 h. Maximum lipase activity under solid-state condition was obtained when Tween 80 at the level of 0.5 % was used as additional carbon source. Best organic nitrogen sources for optimal lipolytic activity was ammonium sulfate (4 %). This study would improve the further optimization steps on the bioprocess development track.
  Asad-ur- Rehman , Sikander Ali and Ikram-ul- Haq
  The effect of different temperatures on citric acid fermentation by Aspergillus niger ANABt was studied in a 15 L stirred fermentor. Ferrocyanide treated (200 ppm) cane molasses medium containing sugar 150 g l-1, was used as the basal fermentation medium. The citric acid yield ranged from 48.58-65.96 g l-1. Maximum amount of anhydrous citric acid obtained during the course of study was 65.96 g l-1 at 30°C. The sugar consumption was 98 g l-1 while mycelial dry weight was 14.55 g l-1. Mycelia were intermediate round pellets.
  Sikander Ali , Ikram-ul-Haq , M.A. Qadeer and Javed Iqbal
  Sixteen different cultures of Aspergillus niger were isolated from different soil samples. These isolates of Aspergillus niger were evaluated for citric acid fermentation in shake flask. Sucrose salt media was used and the volume of fermentation medium was kept at 25 ml. The cultural conditions such as pH (3.5), temperature (30°C), incubation period (8 days) and sugar concentration (15%), were optimised.
  Ikram-ul- Haq , Amna Ehsan , Waseem Ahmad Butt and Sikander Ali
  The optimization of cultural condition was studied for the production of xylanase by mutant strain of Aspergillus niger GCBMX-45. Submerged fermentation was carried out in 250 ml Erlenmeyer flasks. Medium containing (%w/v) NaNO3, 0.1; Tween-80, 0.2; NH4Cl, 0.1; KH2PO4, 0.1; MgSO4.7H2O, 0.03; CaCl2.2H2O, 0.1; wheat bran, 2 was used for xylanase production. The enzyme production was maximum (195 U ml-1) at pH 4.5. One percent meat extract was added in medium as an additional organic nitrogen source.
  Sikander Ali , Asad-ur- Rehman and Ikram-ul- Haq
  The present investigation deals with the time course study of three different mutant strains of Aspergillus niger (IFS-5, IFS-6 & IFS-17) during citric acid production by surface culture technique. The mutant IFS-17 was found to be the best producer of citric acid (76.22 g l-1, 7 days after inoculation) having high production yield coefficients (i.e., Yp/s 0.492 g g-1 & Yp/x 1.992 g g-1). The product is low volume and high cost product. Thus the use of mutant IFS-17 in citric acid fermentation is economically more feasible due to larger citric acid production vs. shorter incubation period.
  Sikander Ali , Asad-ur- Rehman , Amna Ehsan , Ikram-ul- Haq and Javed Iqbal
  The present investigation deals with the effect of vegetative inoculum on submerged citric acid fermentation by Aspergillus niger using stirred bioreactor. All fermentations were carried out following growth on 15% raw molasses sugars for 144 h. Ferrocyanide (200 ppm) was used to control the trace metals present in the molasses medium. The maximum production of citric acid was obtained when 1.0% vegetative inoculum was used. The dry cell mass and sugar consumption were 18.5 and 96.55 g l-1, respectively. The mycelia were intermediate round pellets in their morphology. The specific productivity (qp = 0.074±0.02a g g-1 cells h-1) was several folds higher than many other workers.
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