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Articles by Shiferaw Demissie
Total Records ( 4 ) for Shiferaw Demissie
  Anbessa Dabassa and Shiferaw Demissie
  Salmonelosis is a leading food borne disease worldwide and Africa at large. Eggs were one of the sources Salmonella for the food. So, this study was aimed to evaluate the traditional knowledge with scientific methods for detection of Salmonella in egg shell and content. Randomly collected egg samples were categorized based upon traditional knowledge and Salmonella metabolic recovery and proliferation of cells were done with primary and secondary enrichment media. From a total of 60 samples analyzed, 31 isolates of Salmonella were detected. Moreover, Conventional biochemical and serological test methods were used to identify the suspected Salmonella. The isolates were checked for their susceptibility to different antibiotics on Muller-Hinton agar in standardized inoculum and disc diffusion technique with 11 Oxoid drug discs. The result of study shows that 71% and 29% of Salmonella isolates were detected from egg shell and contents, respectively. All the isolates were resistant to ampicillin; Amoxicillin, Tetracycline and also 45% of the isolates were susceptible to Gentamicin and 16% for ciprofloxacin. Most Salmonella isolates show multi-drug resistance for at least five antibiotics. The laboratory results were supported the traditional egg spoilage identification knowledge, hence most of Salmonella isolates were detected from movable, floating and opaque eggs. On the other hand, the detection of high number of Salmonella isolate from the egg shell could be due to poor sanitation of the laid egg environment. Hence, the appropriate care and awareness should be needed before and/or after the egg laid to minimize contamination of egg by Salmonella.
  Shiferaw Demissie , Diriba Muleta and Gezahegn Berecha
  The aim of this study was to evaluate effect of phosphate solubilizing bacteria on seed germination and seedling growth of Faba bean (Vicia faba L.) under lath-house condition. Phosphorous is an essential macronutrient next to nitrogen required by the plants for vital biosynthesis. But often unavailable for plants because of adsorbed by Ca2+, Mg2+, Fe2+ and Al3+ ions through legend exchange. Although there is mounting information that phosphorus solubilizing bacteria as inoculants increases P uptake of plants. This was not yet tested on Faba bean in Ethiopia. A total of 183 phosphate solubilizing bacteria were isolated from 150 samples collected from rhizosphere soil and root nodules. From these isolates based on their solublization index and mobilization efficacy, two isolates (JURB48 and JURMB69) were selected and tested on Faba bean. The co-inoculants (JURB48+JURMB69) increased the percentage of seed germination (21.4%), vigor index (46.2%), radicle (25.3%) and plumule (50%) lengths of germinated seeds of Faba bean over the non-inoculated. Faba bean shoot fresh, root weight, leaf number, flower number, root dry weight and total dry matter were significantly increased compared to non-inoculated as a result of co-inoculants (JURB48+JURMB69). Plant height, root length, phosphorus content, P uptake and Nodule number and weight were enhanced due to inoculation with JURB48 and JURMB69, respectively, compared to non-inoculated either in the presence or absence of phosphate sources. The present study suggests the potential of JURB48 and JURMB69 isolates as biofertilizers for Faba bean cultivation.
  Shiferaw Demissie , Geda Kebede , Diriba Muleta and Anbessa Dabassa
  Awetu is the River which passes through Jimma town of south western Ethiopia, and one of the major water resources for irrigation and domestic activities for the area. Improper utility of contaminated water create unlimited health concern for the society that utilizing the water for farm and domestic activities. This study was important to evaluate the microbial load, protolytic and lipolytic activities of the isolated microorganisms. The results showed great number of bacterial contaminant in the river. Mainly, Aerobic mesophilic, Enterobacteriaceae, Staphylococcus and aerobic spore formers bacteria range between 103-106 CFU 100 mL-1 while yeast and mold ranged between <30 to 103. In a total of 30 samples were analyzed for microbial load determination using conventional culture method. The results of the this study indicate that the human and cattle feces, domestic activities, as well as solid and liquid waste disposal around the river were the main sources of contamination potential of water. Therefore, continuous and accurate assessment of the quality of water had a paramount importance. In addition, the presence of fecal contaminants in the three sites along the river segment indicates the problem of the water quality. Possible remedial actions are needed as recommendations in this study.
  Shiferaw Demissie and Anbessa Dabassa
  Water quality is the great public health concern in developing countries and for the study area at large. This study is aimed to evaluate the Physico-chemical and antibiotic resistance activities of some bacterial isolates obtained from Awetu River. In this study, 284 bacterial strains were isolated and evaluated for their antimicrobial resistance patterns. All of the isolates tested were not sensitive to Nor, Chl, Kan, Gen and Tet. Among the bacterial isolates, the most frequent resistance was noted for methicillin (96%), followed by resistance to penicillin (85%). The most frequent resistance among (Stapylonocci and Micrococci isolates was observed for Methicillin and Penicillin and streptomycin (96% each), followed by resistance to kanamycin (84%), gentamicin (55%) and methicillin (30%). A total of 9 Multiple Drug Resistance (MDR) patterns were detected. About 42% of the isolates showed MDR to four drugs, 29% to five drugs and 2% to six drugs. The most frequent MDR pattern was Van/Str/Kan/Gen/Met and was seen in 42% of the isolates. This pattern was seen in Lactobacillus, Pediococcus and Weissella isolates, but was the most frequent pattern in Pediococcus isolates (41%). The results obtained from physico-chemical and antimicrobial susceptibility patterns of the isolate were the health risk for the study area community. The study also found the coliform contamination to be the key problem with drinking water.
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