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Articles by Sher Hassan
Total Records ( 4 ) for Sher Hassan
  Mohammad Arif and Sher Hassan
  Pathogen-derived resistance strategies using coat proteins, movement proteins, non-structural proteins. replicases, antisense and satellite RNAs for the production of virus-resistant transgenic plants are briefly discussed. The concept of recombination of viral RNA with transgene products is critically analyzed and potential risks associated with commercialization of transgenic plants with viral inserts and its effects on bio-safety, are highlighted.
  Mohammad Arif and Sher Hassan
  Soybean Mosaic potyvirus (SMV) is highly prevalent in major soybean growing areas of the NWFP. Infected plants exhibited predominantly mosaic and mottling symptoms on leaves and stunted growth due to shortened petioles and internodes. Incidence of the virus ranged from mild to severe infection between 8-72% in areas surveyed. ELISA test further confirmed the incidence of the virus from 7 to 64% in major soybean growing areas of the province, during summer 1997-98. Twelve soybean mosaic virus isolates were collected from soybean crop grown in different areas on the basis of characteristic and distinguishable mosaic and mottling symptoms. Isolates, SMV-S1, S2, S3 from Swat and SMV-P1, P2, P3 from Peshawar were further selected on the basis of severity of symptoms and characterized. SMV-S1, 52, 53 produced severe mosaic on mechanically inoculated soybean (Glycine max) cv. Weber-84, leaf chlorosis and chlorotic local lesions on Phaseolus valgaris and P. lunatus and local lesions on Chenopodium amaranticolor and C. quinoa. SMV-P1, P2 and P3 isolates differed from Swat isolates by producing mild mosaic on soybean cv. Weber-84. Isolate SMV-S1 produced severe mosaic, followed by severe mottling, necrosis and occasionally death of plants Weber-84 inoculated with serially diluted sap from infected soybean plants while SMV-P1 caused mild to moderate mosaic symptoms. The dilution end points of both isolates was 11512. Mean A405,,,,, ELISA values of SMV-S1, 52 and S3 was 1.919 and of SMV-P1, P2 and P3 was 1.377, respectively, after overnight incubation of antigen with substrate solution at 4°C. Among these isolates, SMV-S1 showed maximum A405,in values (1.987) followed by SMV-P1 (1.477), respectively.
  Jahangir Khan , Shabir Ahmad and Sher Hassan
  Rotted apples collected from six cold stores and two fruit markets in Peshawar and Swat divisions showed attacks of Penicillium expansum (74.2%), Alternaria alternata (11.6%) and Rhizopus sp. (14.2%). However, P. expansum was found to be the most prevalent fungus both in apple stores and markets because of its survival potentiality both at low and high temperatures. These fungi are mostly wound pathogens and require injury for infection. The apple variety Red delicious was more susceptible to the disease than Golden delicious indicating differential reaction of these varieties to apple rot. However, the amount of rotting incited by Rhizopus sp. was greater than the other fungi due to its rapid growth and virulence at 20°C.
  Mohammed Arif , Asad Ali , Mouzam Stephen and Sher Hassan
  None of the 29 soybean cultivars and 40 germplasm lines tested were found immune to two isolates of soybean mosaic potyvirus (SMV-S1 and SMV-P1). Malakand-96 was the only cultivar found highly resistant to both S1 and P1 isolates. Swat-84, Bryan, Hobbit-87, Kingsay, Lugan, Sherman and Harper-87 were resistant to S1 and P1 whereas Rincondita was resistant to S1 and moderately resistant to P1 isolate. Similarly, Clark, Nare, NARC-V and Wahab-93 were resistant to P1 but not to S1 isolate. Wahab-93, Kharif-93, Ajmeri, Hodgson, Mid-Spray, Full-Walter, NARC-II, NARC-IV, NARC-VI, Clark and Nare were moderately resistant to S1 whereas Kharif93, Ajmeri, Hodgson, Full-Walter, NARC-Il, NARC-IV, Rincondita, Mid-Pharoah, NARC-VI and Winchester were moderately resistant to P1 isolate. William, Rawal-I, Mid-Pharoah, Bass, NARC-lll, NARC-I, NARC-V and Winchester were susceptible, to SMV-S1 whereas William, Rawal-I, Mid-Spray, Bass, NARC-I and NARC-III were susceptible to P1, Weber-84 was highly susceptible to both isolates of SMV. Among 40 soybean lines, GC-81083-63, GC-81084-51, GC-80072-2-6, AGS-253 and AVRDC-12, AVRDC-13 and AVRDC-15 were highly resistant to both isolates of SMV. Lines GC-81075-44, GC-81090-108, GC-81084-147, GC-81090-10, AGS-85, AGS-249, AVRDC-10, AGS-297, AVRDC-7 were resistant to S1 and P1 isolates whereas L-85-2308, AVRDC-5, AVRDC-14, were resistant to S1 and moderately resistant to P1 isolate. Line L-77-1863 was resistant to P1 but moderately resistant to S1 isolate. Similarly, GC-81080-13, GC-82117-8, AGS-154, L-88-8502, AVRDC-3, AVRDC-4, AVRDC-8 were moderately resistant to S1 and P1 isolates and AVRDC-11, GC-81084-134, GC-8108441, GC-81080-36, GC-81090-94, AVRDC-2, AVRDC-6 were moderately resistant to only P1 isolate. Ten soybean lines were susceptible and 3 highly susceptible to Si whereas 4 lines were susceptible and only one line was highly susceptible to P1 isolate.
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