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Articles by Shahida Hasnain
Total Records ( 12 ) for Shahida Hasnain
  Habib Ahmad , Shahida Hasnain and Afzal Khan
  Reports regarding the chromosomal architecture of Brassica genomes and genome relationship among various species of the genus appeared in the second quarter of the 20th century AD. In the second quarter genome relationship was elaborated through secondary associations, pachytene and somatic karyotypes. Genome analysis and preferential pairing were extensively utilized for characterizing Brassica genomes in the third quarter. In last quarter of the century molecular characterization supplemented the conventional analytical tools for understanding the infrastructure of Brassica genomes. Whether it is the breeding system analysis, chromosome morphology, meiotic associations or molecular characterization proves that Brassica is monophyletic origin and it descended from an unknown six chromosomal prototype. Scientific developments made in Brassica genetics, from genome analysis to genomics during the 20th century AD are generalized in this paper.
  Muhammad Faisal and Shahida Hasnain
  Sikander Sultan and Shahida Hasnain
  Ten hexavalent chromium resistant bacterial strains SECr-1, SECr-2, SECr-3, SECr-4, SECr-5, SECr-6, SECr-7, SECr-8, SECr-9 and SECr-10 were isolated horn the effluents of three electroplating units situated in Gujranwala. These bacteria exhibited a very high level of resistance to hexavalent Cr salt and could bear more than 40 mg ml–1 of potassium chromate in nutrient agar medium. All of them had yellowish white (except SECr-7 which had yellowish brown, SECr-6 and SECr-8 which had off white), convex and circular colonies with entire margins. The cells of these strains were G-ve, motile, aerobic rods. Their morphological and biochemical attributes align them with family Pseudomonadaceae. The optimum temperature for the growth of these bacterial strains was 37°C both in the absence and presence of chromate except SECr-7 fin the absence), SECr-1, SECr-2 and SECr-5 (in the presence of chromate) which yielded maximum growth at 28°C. All of them were capable of growing in wide pH range (pH 5 to pH 9) with maximum growth at pH 7 or 8. However, in the presence of chromate in the medium they preferred alkaline pHs (pH 8 or 9). These bacterial. strains also conferred resistance against salts of other metals and antibiotics. These bacterial strains had great potential for hexavalent chromium reduction and can be exploited for hexavalent chromium detoxification.
  Saima Mehr , Shazia Afrasayab and Shahida Hasnain
  Bacterial strains obtained from the rhizoplane, histoplane and phylloplane of Achyranthus aspera (RAa1, RAa2, HAa1, HAa2, HAa3, PAa1, PAa2, PAa3, PAa4, PAa5, PAa6), Euphorbia heliscopia (REh1, REh2, HEh1, HEh2, PEh1, PEh2), and Malvestrum tricuspidatum (RMt1, HMt1, HMt2, PMt1, PMt2) were used to inoculate the seeds of Triticum aestivum var. Inqlab-91. Inoculated as well as uninoculated seeds were germinated and grown under 0 and 100mM NaCl stress for 10 days. Different growth parameters and some biochemical characters were considered for these studies. Salinity reduced the percentage germination, root, shoot, seedling length as well as fresh weight per seedlings, whereas it increased the dry matter accumulation, Na+, K+ uptake, protein, auxin content and activity of peroxidases and acid phosphatases in uninoculated seedlings. While the association of many bacterial strains with wheat enhanced the seedling growth under 0 and 100mM NaCl stress. Bacterial inoculations improved different growth parameters by reducing the Na+ uptake and improving the protein content, auxin production and alteration in enzyme activities.
  Tanveer Zahra Mirza and Shahida Hasnain
  Curing efficiency was higher with different TMP concentrations in combination with alkaline pH (9 ) at 37 as well as 42°C. Both strains could tolerate up to 3.5 M NaCI in L agar but in L broth RCa-1 could deter 2 M and Ca-2 upto 2.5 M NaCI. Curing was accomplished with loss of salt tolerance property. Salt tolerance among the cured derivatives ranged from 0.1 M (majority) to 0.5 M (few) in L broth. Generally, Na+ ion uptake was more in the cured derivatives as compared to their parental strains when grown in 0.1 M Neel supplemented broth under optimum temperature and pH. A comparison of cell wall composition of parental strains and their cured derivatives exhibited more peptidoglycan (PG), diaminopamelic acid (DAP) and teichoic acid contents in the plasmid free isolates.
  Azra Yasmin and Shahida Hasnain
  Bacterial strains (which could tolerate 2-3 M NaCI in the growth medium) were isolated from the roots of Erigerane linifolious. They were EI-1, EI-2, El-3 (from histoplane of roots) and REII-1, REI-2, REI-3, REI-4, REI-5 (from the rhizoplane). All bacterial strains were motile rods (except El-2 which were cocci), exhibiting either Gram-negative (El-1, El-2, El-3, REI-1) or Gram-variable (REI-2, REI-3, REI-4, REI-5) staining. These strains were affiliated with genus De/ya (EI-1), Vibrio (EI-3, REI-1), while four Gram-variable strains chaired characters with Bacillus pumilus. They have wide temperature and pH ranges with different optima. These strains also exhibit multiple salts/osmolytes/heavy metals/antibiotics resistance. Curing of plasmids from four of these strains revealed that salt tolerance and most of the other resistances were plasmid encoded. Plasmids residing in these halo-tolerant strains were conjugative (except pSH1413I. Hybridization experiments revealed that one of these plasmids (pSH1414) belonged to IncN group of plasmids.
  Shazia Afrasayab , Azra Yasmin and Shahida Hasnain
  Among six Hg-resistant isolates (SHg-7, SHg-8, SHg-9, SHg-10, SHg-11, SHg-12), SHg-8 and SHg-12 could tolerate 450 μg ml–1 of HgCl2 in L-agar medium, whereas rest of the strains could tolerate 500 μg ml–1 of HgCl2 in this medium were Gram-negative rods (SHg-7, SHg-8, SHg-9, SHg-10, SHg-11) and SHg-12 were Gram-positive rods. SHg-11 & SHg-12 were strictly aerobic, remaining were facultatively aerobic. On the basis of morphological and biochemical characterization isolates SHg-7, SHg-8, SHg-9, SHg-10 could be affiliated with family Vibrionaceae and SHg-11 shared characters with Pseudomonadaceae, while the affinities of SHg-12 remained uncertain. All of them had a single plasmid band except SHg-11, but these plasmids were non-conjugative. They had broad pH range (5-11) with pH optima either at 6 or 7. Their optimal growth temperature was 32°C (SHg-9, SHg-10, SHg-11) and 37°C (SHg-7, SHg-8, SHg-12). All of them were sensitive to Sm, Km and Tc, while they showed resistance against Ap and Cm. They could also tolerate other heavy metal salts.
  Muhammad Faisal and Shahida Hasnain
  Shaiza Iftikhar , Muhammad Faisal and Shahida Hasnain
  In the present study chromium resistant bacterial strain Bacillus cereus S-6 isolated from effluents of tannery was used for the reduction of toxic Cr (VI) into less toxic Cr (III). It could resist very high concentration of K2CrO4 i.e., up to 40 mg mL-1 on nutrient agar, 25 mg mL-1 in nutrient broth and up to 1.5 mg mL-1 of K2CrO4 in acetate-minimal medium. At an initial Cr (VI) concentration of 100 μg mL-1, the cytosol and membrane preparation of the strain were able to reduced almost 67 and 43% of Cr (VI) within 24 h incubation period while the heat killed cytosol and membrane preparation reduced 24 and 18% within the same time period. At high initial K2CrO4 concentration (500 μg mL-1), the reduction percentage decreased and cytosol reduce 36% and membrane preparation 18% of the total chromium supplied after 24 h. After heat shock these reduction values were 13 and 9%, respectively.
  Khushi Muhammad , Azra Yasmin , Hazir Rehman , Muhammad Faisal and Shahida Hasnain
  Twenty-four bacterial strains were isolated from rhizosphere and histoplane of four different arid plants, Trianthema partulacastrum, Rumex dentatus, Chenopodium morale and Coronopus didymus. These bacteria were inoculated to seeds of Vigna radiata var NM-92 which were germinated and grown for 15 days. Majority of strains had inhibitory effect on seed germination (2.2 to 13.0%). The increase in seedling length ranged from 17 to 38% with different inoculations. Bacterial strains TP1, TP3, TP4, RTP4 and RRD1 were the most effective ones for the different growth parameters such as seed length and seed weight whereas the most adverse effects were recorded with the inoculation of TP17 strain. Maximum increase in protein and auxin content was observed with strains TP5 and RCM2, respectively. Peroxidase and acid phosphatase showed maximum activity with the inoculation of strain TP1.
  Saira Anwar , Anjum Nasim Sabri , Hazir Rehman , Muhammad Faisal and Shahida Hasnain
  Effects of temperatures (37 and 45°C) in combination with varying pH (6, 7, 8 and 9) after 24 and 96 h of incubation were studied on soluble protein content and protein profile of PY79 (wild type) and sporulation defective mutant strains (5, 19, 96 and 99) of Bacillus strains. These strains were isolated from local polluted environments around Lahore, Pakistan. Soluble protein content is more in PY79 as compared to the defective sporulating mutant strains (5, 19, 96 and 99). In strain 5, it appeared that polypeptides of 55-59 kDa (pH 6, 7 and 8), 50-54 kDa (pH 6, 7 and 8), 45-49 kDa (pH 8), 20-24 kDa (pH 7) and 10-14 kDa (pH 8) were the heat stress proteins. While in strain 99, at high temperature different stress proteins appeared especially at pH 6 (30-34, 25-29 kDa), pH 7 (25-29 kDa), pH 8 (50-54, 30-34 kDa) and at pH 9 (45-49, 30-34, 20-24 kDa). At temperature 37°C, the polypeptides of 55-59 kDa at pH 6, 7 and 9 were observed which disappeared at high temperature.
  Muhammad Faisal and Shahida Hasnain
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