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Articles by Sarmin
Total Records ( 4 ) for Sarmin
  Trini Susmiati , Prabowo Purwono Putro , Sarmin , Claude Mona Airin , Hartiningsih and Pamungkas Bagus Satria
  Background and Objective: Polymorphisms of kappa-casein protein genes are closely related to the quality of milk production and composition. The purpose of the study was to investigate polymorphisms in genes encoding kappa-casein (κ-casein) milk protein produced by the dairy cattle of KP4/PIAT UGM Yogyakarta and BBPTU-HPT Baturraden. Materials and Methods: Twenty-three cattle were used to investigate kappa-casein polymorphisms in dairy cows from two different animal farms. Nine cattle were distributed from BBPTU-HPT Baturraden, Central Java and 14 cattle were allocated from KP4/PIAT UGM Yogyakarta. The research procedures included blood sampling, DNA extractions and DNA amplification using Polymerase Chain Reaction (PCR). Kappa-casein gene PCR products were analyzed by using the PCR-RFLP technique with restriction enzymes Hinf I and Hind III. Results: The results of restriction digestion using the Hinf I showed four restriction fragments in the blood sample from BPPTU-HPT Baturraden and three restriction fragments from KP4/PIAT UGM Yogyakarta. The digestion of PCR product using Hind III produced one restriction fragment in the blood samples from KP4/PIAT UGM Yogyakarta and 4 fragments in the samples from BPPTU-HPT Baturraden. Conclusion: The dairy cattle of BPPTU Baturraden offer a more favorable genetic selection of animals for milk production and industry due to their genetic variants in terms of genotypes and alleles.
  Sarmin , Irkham Widiyono , Pudji Astuti and Prabowo Purwono Putro
  Background: The ability of ruminants to undergo metabolic and endocrine adaptation to feeding restrictions and refeeding varies depending on the species and physiological characteristics, the age at which the feed restriction and refeeding are imposed, the rate and length of the feed restriction and refeeding, the physiological responses of various body parts to the feed restriction and refeeding conditions, sex and breed. Objective: The objective of this study was to evaluate the metabolic rates and endocrine responses of Kacang goats, Indonesian-bred ruminants, during the restricted feeding and refeeding periods. Methodology: Nine 1.5-2.5 year-old male goats were subjected to feed adaptation for the first 60 days. Then, the goats were subjected to three-level feed restriction for the second 60 days. The goats received 40, 50 or 60% feed restriction of dry fodder. The goats allocated to the 40, 50 or 60% feed restriction groups were referred to as group 1, 2 and 3, respectively. For the final 60 days, the goats underwent 100% refeeding with dry fodder. The weights of the ruminants were measured and blood samples were obtained at the end of the feed adaptation, feed restriction and refeeding. Results: At the end of the 60-day restriction, the three goats in group 3 were less than the baseline of Average Daily Gain (ADG), while the six other goats in groups 1 and 2 were still greater than the baseline of ADG, although there were no significant differences among the groups (p>0.05). The feed restriction increased lipolysis in adipose tissue and protein mobilization, non-esterified fatty acids (NEFAs) and albumin as the carrier of NEFA, creatinine and total protein in serum. Nonetheless, there were no differences among the three groups (p>0.05). Thyroid hormones (T3 and T4) as lipolytic hormones were ignored because their rates were lower than the baseline. Lipolytic responses in the form of High Density Lipoprotein (HDL) occurred in the three groups and there were significant differences among the three groups (p>0.05), indicating that triglycerides exported as Low Density Lipoprotein (LDL) were also not different among the three groups (p>0.05). This finding showed that there were no LDL secretion differences among the three groups. The two metabolic ketogenic effects, namely, Blood Urea Nitrogen (BUN) and glucose showed that BUN decreased to less than the baseline in group 2, although there were no significant differences among the groups (p>0.05). The glucose rate in group 2 was the highest among the groups (p<0.05), likely because of the low catabolism of labile protein deposits in group 2. After the refeeding was completed, the ADG in groups 2 and 3 increased significantly (p<0.05). In contrast, the ADG in group 1 did not increase significantly (p>0.05). Lipolysis continued to occur after the refeeding, with high rates of NEFA and albumin in the three groups without any differences among the groups (p>0.05). The lipolysis of hormones T3 and T4 was ignored, with both hormones remaining low. The rates of HDL and triglycerides exported as LDL were also constant and were not different among the three groups (p>0.05). Creatinine decreased significantly in the three groups (p<0.05) and there were no differences among the groups. The total protein increased significantly (p<0.5), but there were no differences among the three groups. The BUN metabolite did not change after refeeding was imposed on the three groups (p>0.05), although the BUN metabolite in group 1 tended to be higher than that in group 2. The glucose rate increased significantly in all three of the groups (p<0.05), although none of them reached the baseline. The ruminants in group 2 possessed the lowest glucose rate (p<0.05). Conclusion: A group of goats with a 50% restriction level of dry fodder tended to use ketones as the source of energy by suppressing protein degradation during feed restriction. The responses of ruminants to refeeding took the forms of continued lipolysis and increased protein degradation.
  Bambang Suwignyo , Zein Ahmad Baihaqi , Ristianto Utomo , Sarmin and Irkham Widiyono
  Objective: This study aimed to determine the effects of different feed restrictions and refeeding on feed intake, digestibility, body weight gain and feed cost in goats. Materials and Methods: There were three treatment groups of goats had an average initial body weight of 20-25 kg. Control goats (R0) were fed 3.5% Dry Matter (DM) of body weight or underwent 1 month of feed restriction treatment (R1) and 2 months (R2) of 60% restrictions (DM base). After the restriction period, R1 and R2 groups were fed ad libitum. The variables measured include Dry Matter Intake (DMI), Organic Matter Intake (OMI), Crude Protein Intake (CPI), body weight gain, Dry Matter Digestibility (DMD), Organic Matter Digestibility (OMD), Crude Protein Digestibility (CPD) and feed cost. Data were analyzed using ANOVA based on a Completely Randomized Design (CRD) on SPSS program version 21 for windows. Results: Feed restriction had a significant effect on body weight gain, the DMI, OMI, CPI and feed cost. However, the treatments did not significantly affect body weight or DM, OM and CP digestibility. Conclusion: One month of feed restriction continued with 2 months of re-feeding (R1) can lead to compensatory growth such that the average daily gain is the highest.
  Irkham Widiyono , Sarmin , Prabowo Purwono Putro and Pudji Astuti
  Background and Objective: Nutritional status of livestock has impact on growth performance and development of reproductive organs. Testicular mass, semen characteristics, spermatogenesis and reproductive hormones can be influenced by feeding levels. This study was conducted to evaluate semen characteristics of Kacang goats given various feeding levels for 24 weeks. Methodology: Nine adult male Kacang goats were divided into 3 feeding groups (G1, G2 and G3). After a 2 week adaptation period, all three groups of goats were fed fully for 8 weeks (full feeding). Then the goats were fed an amount that was 60% (G1), 50% (G2) or 40% (G3) of full feeding levels for the next 8 weeks (restricted feeding). Finally, the goats were refed fully for the last 8 weeks (re-alimentation). Semen samples were collected using an artificial vagina at the end of each feeding period. Results: Full feeding followed by restricted feeding and resumption of full feeding had no significant effects on the ejaculate volume, sperm cell concentration, sperm cell motility and proportion of live sperm values in Kacang goats. Semen parameters were maintained in the physiological range that was normal for Kacang goats. Conclusion: Kacang goats have a good capacity to preserve semen quality at various levels of feeding.
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