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| Articles
by
Santosh Chokkakula |
Total Records (
1 ) for
Santosh Chokkakula |
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Santosh Chokkakula
,
Kalyani Dasari
,
Idress Hamad Attitalla
,
Srikantam Aparna
,
Padmaja Ponnada
and
Madan Mohan Male
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The main pathogen based molecular markers involved in strain typing and strain
differentiation are SNP and VNTR. A typing system for global differentiation
of M. leprae is the analysis of variable number tandem repeats based
on STRs. To expand the analysis of M. leprae TTC gene polymorphism, this
study involved strain typing of 14 Lepromatous Leprosy (LL) SSS samples. For
the strain typing and strain differentiation of M. Lprae, the present
study involves 14 bacteriological positive Lepromatous Leprosy (LL) Slit Skin
Smear (SSS) samples. Most of cases were identified as male and 13 cases have
associated with nerve involvement. Two cases with tenderness and two with eye
involvement also have been screened. DNA was extracted from SSS of 14 untreated
LL leprosy cases. PCR amplification of TTC gene and sequencing of amplicons
showed the allelic variation and percentage of distribution of allele in the
enrolled samples. Among the 14 SSS samples, 1 (7%) exhibited 9 copy number,
1 (7%) associated with 10 copy number, 6 (43%) identified as 12 copy number,
1 (7%) known as 13 copy number, 1 (7%) exhibited 15 copy number, 1 (7%) linked
with 16 copy number, 1 (7%) exhibited 18 copy number and 2 (15%) identified
as 19 copy number of (TTC) 21 allele. However, the positive strain called TN
was associated with standard repeat units of TTC that is 21. As conclusion we
found that, the present results has demonstrated that TTC repeats of leprosy
patients has 12 and 19 copy number as a abundant which was slight differ from
other leprosy strains from India. |
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