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Articles by Samy Ali Hussein
Total Records ( 3 ) for Samy Ali Hussein
  Samy Ali Hussein , Mohamed Ragaa R. Hassanein , Aziza Amin and Asmaa H. Mohammad Hussein
  Objective: The protective effects of alpha-lipoic acid against lead (Pb) induced oxidative stress to kidney tissues in rats were evaluated. Materials and Methods: Eighty male albino rats were divided into 4 equal groups. Group 1: (control) administered distilled water. Group 2 received lead acetate orally (30 mg kg–1 b.wt., of 1/20th of LD50). Group 3 received lead (30 mg kg–1 b.wt.) plus alpha-lipoic acid (54 mg kg–1 b.wt./day/i.p). Group 4: received alpha-lipoic acid (54 mg kg–1 b.wt.). Blood samples were collected for determination of serum TNF-α, IL-6, IL-1β. Also, kidney tissues were taken and processed for L-MDA, CAT, SOD,GPx, DNA fragmentation, caspase-3, NF-kB P65, 8-OhdG and Cox-2. Additionally, liver and kidney specimens were excised for histopathological examination and lead residues determination. Results: The obtained results showed significant increase in serum TNF-α, IL-6 and IL-1β, kidney tissues L-MDA, DNA fragmentation, caspase-3, NF-kB P65, 8-OhdG and Cox-2 in addition to liver and kidney lead residue in lead intoxicated rats. However, administration of alpha-lipoic acid exhibited a significant decreased in all mentioned parameters. Kidney tissues antioxidant enzymes were markedly decreased in lead intoxicated rats and the activities were attenuated after treatment with alpha-lipoic acid. Various pathological alterations were observed in liver and kidney of lead administered group. Interestingly, the histopathological results supported that alpha-lipoic acid markedly reduced the deleterious effect induced by Pb and preserved the normal histological architecture of the liver and kidney tissues. Conclusion: The results indicate that, alpha-lipoic acid could be applicable as a cytoprotective against oxidative stress of tissue damage mediated by heavy metals intoxication as confirmed by biochemical and histopathological results.
  Samy Ali Hussein , Mamdouh El-Haggar , Omayma Ahmed Abo-Zaid , Mohammed Ragaa Hassanien and Ragab El-Shawarby
  Diabetic neuropathy is the most common complication of diabetes. We investigate the effect of alpha-lipoic acid and insulin alone and in combination on changes in the phospholipids composition in the sciatic nerve of experimental diabetic neuropathy. A total of 120 male rats were used in this study. The experimental induction of diabetes in rats was induced by a single intraperetinoel (i.p) injection of 50 mg kg-1 of streptozotocin (STZ) freshly dissolved in citrate buffer, pH 4.5. After eight weeks of diabetes induction all rats were divided into six main equal groups, 20 animals each. Group I (control group): Received no drugs, Group II (diabetic group), Group III (normal α-lipoic acid-treated group), Group IV (diabetic alpha-lipoic acid -treated group), Group V (diabetic insulin- treated group), Group VI (diabetic alpha-lipoic acid and insulin-treated group). Eight weeks after diabetes induction therapeutic treatment with alpha-lipoic acid (54 mg kg-1 b.wt. i.p daily) and insulin (2U s.c daily) were given either alone or in combination and continued for six weeks. Equivalent volumes of saline were given subcutaneously to the rats in the other diabetic and non diabetic control groups. Blood samples and sciatic nerve tissues were collected at 4 and 6 weeks from the onset of treatment for determination of serum glucose and total cholesterol, total phospholipids and membrane phospholipids composition of sciatic nerve. The obtained results revealed that, diabetic neuropathy in rats resulted in marked increase in serum glucose level, sciatic nerve total cholesterol and phosphatidylglycerol contents. Treatment with α-lipoic acid significantly decreased serum glucose, phosphatidylglycerol and sphingomyelin contents with increase in total cholesterol content in sciatic nerve. Insulin treatment significantly increased total phospholipids and markedly decrease phospholipids composition of rat sciatic nerve including phosphatidylethanolamine, phosphatidylcholine, phosphatidylglycerol and sphingomyelin contents. Meanwhile, treatment with α-lipoic acid and insulin combination significantly decreased total phospholipids concentration and phospholipids composition in rat sciatic nerve including phosphatidylcholine, phosphatidylglycerol and sphingomyelin contents. These results indicate that, alterations in the amounts of phospholipids composition in sciatic nerve could be related to the physiological changes of early diabetic neuropathy. These result suggest that, administration of α-lipoic acid combined with insulin prevent hyperglycemia-induced changes in phospholipids composition suggesting its therapeutic potential in complications of diabetes and dibetes neuropathy.
  Samy Ali Hussein , Ahmed Ragab Omayma and Asmaa Elwakil
  Brain stroke is the rapid loss of brain function due to disturbance in the blood supply to the brain. So, it is important to test the biochemical abnormalities and oxidative mechanisms of focal cerebral ischemia induced in left common carotid artery occlusion (LCCAO).The rats were divided into five groups. Group 1 (Control normal), group 2, 3, 4 and 5 were subjected to occlusion for ½, 1, 4 and 6 h occlusions, respectively. LCCAO was retracted to allow reperfusion of ischemic region. Blood samples and tissue specimens from brain were collected four times after reperfusion, zero time, 1, 3 and 24 h, respectively. Moreover, the changes in blood and brain biomarkers in ischemia/reperfusion injury were assayed. The results showed that LCCA occlusion in male rats significantly increased the levels of brain L-MDA, lactate, sodium, antioxidant enzymes, AChE, LDH and plasma fibrinogen. Also brain stroke significantly decreased the levels of brain nitric oxide, CAT and serum CK and C-RP as compared to control rats. Moreover, reperfusion of LCCAO significantly increased levels of brain L-MDA, antioxidant enzymes and serum C-RP. While, results showed a significant decrease in brain nitric oxide, sodium, AchE, serum CK as well as fluctuation in brain LDH, lactate and plasma fibrinogen levels as compared to the zero hour post occlusion. In conclusion, the results provide in vivo evidence that brain ischemia has harmful effect on brain energy metabolism, through induction of oxidative stress via production and rapid increase in the generation of reactive oxygen species, lipid peroxidation and alteration in antioxidant defenses.
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