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Articles by Sabrina
Total Records ( 5 ) for Sabrina
  Yurnalis , Husmaini and Sabrina
  Objective: The objective of this study was to determine Growth Hormone (GH) gene polymorphisms and their association with productive traits, including body weight, at ages 1, 2, 3, 4, 5, 6, 7 and 8 weeks. Methodology: Polymorphisms in exon 1 of the GH gene were evaluated in two duck populations in West Sumatra Province Indonesia (Pitalah and Kumbang Janti ducks). For this purpose, blood samples were collected and DNA samples were extracted using the Promega Wizard® Genomic DNA Purification Kit. For this purpose, a total 225 ducks blood samples were collected from 145 male and 80 female ducks. Genetic polymorphisms were determined with the Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) method using the Eco721 restriction enzyme and agarose gel electrophoresis. Direct sequencing of some samples was used to confirm the results. Results: Two alleles (GHG and GHA) and three genotypes (GH/GG, GH/GA and GH/AA) were found in the studied duck samples at locus GH/Eco721. In both groups of ducks, the dominant allele was GHG. The most frequent genotype in the examined ducks was GH/GA. Three genotypes were observed in the Pitalah ducks, whereas two genotypes (GH/GA and GH/GG) were identified in the Kumbang Janti ducks and in the males. Pitalah ducks with the GH/GA genotype were characterized by a higher (p<0.01) body weight than the ducks with the GH/GG and GH/AA genotypes. This same trend was observed in the female Pitalah ducks; individuals with the GH/GA genotype had higher body weights (p<0.05 and p<0.01) than the birds with the two other detected genotypes. Kumbang Janti ducks with the GH/TT genotype were distinguished by higher values of all evaluated traits compared to the ducks with the GH/CT and GH/CC genotypes; however, most of the recorded differences were not significant. The only trait that was markedly impacted (p<0.05) by the polymorphism of GH gene intron 1 was the body weight at 5, 6, 7 and 8 weeks. Conclusion: This study found that the GH/TT genotype was associated with a higher body weight at 5, 6, 7 and 8 weeks of age in Pitalah and Kumbang Janti ducks.
  Nuraini , Sabrina and Suslina A. Latif
  An experiment was conducted to improve the nutrient content of tapioca by product to produce β carotene rich feed as alternative poultry feed through fermentation by using carotenogenic fungi (Neurospora crassa) as inoculums. The experiment was determination of substrate composition (carbon source and nitrogen source) based on nutrient quality and quantity of these fermented products. The study was conducted in experimental methods, using the completely randomize design in factorial with 2 treatment were : 1. A factor, ( tapioca by product as carbon source with nitrogen sources: A 1 = tapioca by product+tofu waste, A 2 = tapioca by product + palm kernel cake and A 3 = tapioca by product + rice bran. 2. B factor (Percentage of composition of carbon source with nitrogen source), B 1 = 90% : 10%, B 2 = 80% : 20%, B 3 = 70% : 30% and 60% : 40%). Results of study showed that optimum substrate composition of the fermentation by Neurospora crassa was the mixture 60% tapioca by product with 40% tofu waste. This conditions can increase β carotene and crude protein and also decrease crude fiber which made the nutritional value of the product based on dry-substance was 295.16 μg/g, β carotene 20.44% crude protein, 2.75% crude fat, 11.96% crude fiber, 0.24% calcium, 0.17% phosphor, metabolic energy 2677 Kcal/kg, 67.05% nitrogen retention and 35.44% fiber digestion.
  Nuraini , Sabrina and Suslina A. Latif
  An experiment was conducted to determine the effect of feeding fermented products by Monascus purpureus on performances and egg quality of laying hen. This experiment was arranged in a completely randomized design with four dietary treatments: 0%, 10%, 20% and 30% fermented products by Monascus purpureus in the diets and five replications. 200 laying hen Isa Brown (22 week of age) were randomly allocated into 4 treatments (5 replications of 10 hens per treatment). Variable measured were feed intake, egg production, feed conversion, egg cholesterol and yolk colour. Results of the experiment indicated that feed intake, egg production, feed conversion, egg cholesterol and yolk colour were affected (p<0.01) by increasing fermented products in the diet. Feed intake, egg production and yolk colour in D treatment (used 30% fermented product by Monascus purpureus) was the highest than other treatment, but the lowest on egg cholesterol and feed conversion. The conclusion of the experiment that up to 30% fermented products by Monascus purpureus improved performance and reduced egg cholesterol 31.49% and increased yolk colour 18.56%.
  Sabrina , M.H. Abbas , E. Purwati , Y. Heryandi and Robby
  This study was conducted to evaluate the effects of altitude and dietary protein level on performance of local ducks. Two hundred and forty of female Pitalah ducks 14 weeks old were used in this study. Ducks reared on high altitude (H) and low altitude (L). The research was carried out by using Split-plot arrangement in a Randomized Block Design with the H and L as main-plot and the dietary protein level (PL: T-1 = 14, T-2 = 16, T-3 = 18% protein) as sub-plot. Treatments were done when duck was 16 weeks old. Observations were made after the production of duck was 10 %. The environment and rectal temperature were recorded daily. Variables such as: feed intake, protein intake, egg production (Duck day and Egg mass) and Feed Conversion Ratio (FCR) were observed weekly. The altitude were highly significantly (P<0.01) influenced on feed intake, protein intake, egg production (Duck day and Egg mass) and Feed Conversion Ratio (FCR). PL were not significant (P>0.05) affect to feed intake but significantly (P<0.05) increased duck day production and highly significant (P<0.01) increased egg mass and FCR. There was no interaction (P>0.05) between altitude and dietary of protein level. The results of this study showed that the performance of the duck raised on the H was better than L. The increasing of protein level up to 18% have been given the best performance of Pitalah duck.
  Husmaini , Sabrina , F. Arlina , E. Purwati , S.N. Aritonang and H. Abbas
  Objective: A trial was conducted to evaluate the effects of age at administration of Lactococcus plantarum isolates from virgin coconut oil processing waste on the number of Lactic Acid Bacteria (LAB) in the intestine and the growth performance of broilers. Methodology: The research used 160 day old cobb broilers divided into 4 treatment groups: T0 (without LP), T1 (Lactococcus plantarum administered at 1 week of age), T2 (2 weeks of age) and T3 (3 weeks of age). The basal diet consisted of corn, rice brain, fish meal, soy bean meal, bone meal, vegetable fat and premix (21.1% crude protein and 3038 kcal kg–1 energy metabolism). Chickens were given Lactococcus plantarum only one time and were slaughtered every week until 5 weeks old. Variables included the number of LAB, E. coli and Salmonella in the intestine, thickness and length of the intestine, carcass weight, fat and cholesterol content of carcass, body weight, feed intake and feed efficiency. The data were evaluated using a one-way ANOVA. Results: The results showed that Lactococcus plantarum administration affected the balance of microflora in the gut and the length of the intestine. Lactococcus plantarum treatment significantly increased the number of LAB in the intestine (p<0.01) up to 2 weeks after administration, conversely, the number of E. coli and Salmonella decreased. When given at 2 and 3 weeks, the effect of Lactococcus plantarum increased intestinal length and broiler growth performance was highly significant (p<0.01). Probiotic treatment did not affect carcass percentage but affected both the abdominal fat and cholesterol of broiler meat. Conclusion: Optimal body weight, feed conversion ratio (1.78) and cholesterol content were observed when Lactococcus plantarum was given at 2 weeks of age.
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