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Articles by S.Z. Mirhosseini
Total Records ( 7 ) for S.Z. Mirhosseini
  H. Kioumarsi , K. Jafari Khorshidi , M. Zahedifar , A.R. Seidavi , S.Z. Mirhosseini and M.R. Taherzadeh
  In this study, two levels of Metabolizable Energy (ME) (2.3 and 2.5 Mcal kg-1 DM) and three levels of Crude Protein (CP) (12, 14 and 16%) and their interactions were studied to identify the optimum levels of dietary energy and protein for lambs of the Taleshi breed. The growth performance variables measured included Average Daily Gain (ADG), final weight, Daily Dry Matter Intake (DDMI, kg day-1) and Feed Conversion Ratio (FCR) whilst other commercially important factors were assessed during specific periods. The urea dilution method was used for predicting the body composition of live lambs. At the end of the experiment, all animals were slaughtered, the carcasses were cut and the chemical composition of the area around the 9th, 10th and 11th rib was measured. The study comprised a completely randomized design with a 2x3 factorial arrangement with four replications per diet. The results showed that the energy and protein levels had a significant effect (p<0.05) on growth. The diet with 2.5 Mcal ME kg-1 and 14% CP was associated with the best final weight, ADG, FCR, feed cost kg-1 gain, un-variable profit kg-1 live weight, un-variable profit/total gain, carcass weight, shoulder weight, thigh weight and ribeye area (REA). A high dietary energy level helps to produce more ME and fermentable products for paunch microorganisms resulting in an increase in the synthesis of microbial protein and therefore the amount of protein available to the animal. Increasing the dietary protein level causes a change in the process of fermentation in the paunch whilst increasing fatty acid production and the ratio of propionate to fatty acids. These changes in the paunch improve the lamb`s energy balance allowing more nitrogen to be stored and increasing the body weight.
  H. Kioumarsi , K. Jafari Khorshidi , M. Zahedifar , A.R. Seidavi , Z.S. Yahaya , W.A. Rahman and S.Z. Mirhosseini
  This study estimated the relationship between Urea Space (US) and carcass attributes in lambs to derive coefficients for these relationships and functional equations for components of carcass quality and quantity. Twenty-four male Taleshi lambs with an average age of 8 months were used. Lamb live weight was determined using the urea dilution method and two or three days before slaughter, the urea dilution procedure was again used to estimate the chemical composition of the carcass. Subsequently, plasma urea nitrogen was determined and the percentage of urea space to lamb live weight was calculated. After slaughter, the carcasses were butchered and the parts measured. The results showed that the average amount of urea injected was 20.09 cm2 and the Blood Urea Nitrogen (BUN) averaged 17.16 and 27.72 mg before and after injection respectively. The average percentage of US to lamb live weight was 18.78%. Correlation coefficients between live weight, empty body weight, hot carcass weight and the weight of different parts of the carcass were high and statistically significant (p < 0.01). The urea space was significantly related to the percentage of protein and ash in the region of the 9th, 10th and 11th ribs (p < 0.05). Using this rib area and US, the development of functional equations between live weight and empty body weight for different parts of the carcass showed that the urea dilution test is a useful tool for predicting the chemical composition of Taleshi lamb carcasses.
  A.R. Seidavi , S.Z. Mirhosseini , M. Shivazad , M. Chamani and A.A. Sadeghi
  In the present study, a multiplex Polymerase Chain Reaction (mPCR)-based assay was developed for the simultaneous identification of Salmonella sp., Escherichia coli and Bifidobacterium sp., that isolated from broiler gastrointestinal in Iran. In order to detect these bacteria species, we were used a set of specific primers that produce different fragment size. Our results showed that all tested gastrointestinal segments were contaminated by these species and therefore the mPCR method is a appropriate method to identify these species directly from poultry gastrointestinal samples. There was no difference in the sensitivity of this method between samples of different gastrointestinal segments. This method has the potential to save considerable time and effort within the laboratory. Our developed mPCR is fast, sensitive, specific and can be used as a reliable choice for routine detection method of these bacteria species in chick gastrointestinal contents.
  M. Salehi Nezhad , S.Z. Mirhosseini , S. Gharahveysi , M. Mavvajpour and A.R. Seidavi
  The present study aims at shedding more light to larval duration and development of silkworm lines from Iranian silkworm gene bank and comparison of the results using statistical models for selection of the superior strains. Feeding and other conditions of larval rearing were conducted following the standard procedure and all germplasm strains were reared under standards protocols in all rearing steps. From obtained results, it is showed that the larval duration of the 101 (608.000 h), 5118x10133-3-3 (588.670 h), 307-300-2 (584.000 h), 105 (584.000 h) and 31 (584.000 h) strains remained significantly at upper level than other strains, respectively. The feeding larval duration in B2-09 (574.000 h), N19 (533.000 h), 1433-9 (525.000 h), BH-2 (517.330 h) and 1433-15 (511.330 h) strains increased significantly in comparison with other strains. Molting larval duration remained significantly at upper level in I 20 (197.670 h), 107-K (113.000 h), Black Larvae-White Cocoon (104.000 h), 101(104.000 h) and Shaki (103.000 h) increased significantly in comparison with other strains. From obtained results, it is showed the 1-3 instars larval duration of the Black-White (292.670 h), 101 (290.000 h), 1003-5 (288.670 h), 101xF6 (286.000 h) and 31 (286.000 h) strains remained significantly at upper level than other strains, respectively. Totally, 7409 (577.881), Black Larvae-White Cocoon (577.508), 236 (570.769), M-1-2(5) (568.583) and T5-M (566.602) showed higher evaluation index values. Also, 7409 (5.374), 236 (5.267), T5-M (5.183), 113-K (5.163) and White Larvae-Yellow Cocoon (5.027) showed higher sub-ordinate function values.
  A.R. Bizhannia , S.Z. Mirhosseini , B. Rabiee and M. Taeb
  The silkworm is an important economical insect in sericultural field. Its major production-economic characteristics are polygenic. In this study, three F2 populations (second generation) derived from three cross between three pairs, parents of Lemon Khorasan (as maternal) and 107 (as paternal) lines. we contacted AFLP (amplified fragment length polymorphism) technique for mapping genetic factors or QTLs that effect on Cocoon Shell Weight (CSW) trait. Following it we used 20 selected primer combinations of PstI/TaqI and DNAs were individually extracted to phenol-chloroform method. They digested by two restriction enzymes (TaqI and PstI) and also produced DNA fragments amplified by appropriate adaptors separately. After transferring of DNAs samples on annealed 6% polyacrylamide gels and genotyping of individuals, the linkage maps of each population were drawed. The QTLs for cocoon shell weight trait in LRS = LRT>12.5 (LOD>2.71) threshold level based on permutation test (n = 1000) and using compound interval mapping methods were analyzed and detected 5, 1 and 1 QTLs that were localized on linkage groups 9, 11; 12 and 24 in each studied F2 populations, respectively. The QTLs had different gene effects from over dominance, dominance to partial dominance.
  M. Salehi Nezhad , S.Z. Mirhosseini , S. Gharahveysi , M. Mavvajpour and A.R. Seidavi
  This study was conducted in ordered to analyze of phylogenetic relationships and determination of relationship amounts in all peanut cocoon strains of Islamic Republic of Iran germplasm based on reproductive characteristics. Hierarchical agglomerative clustering was done by using NTSYS-pc, version 2.02e based on complete, single, UPGMA, UPGMC, FLEXI approaches and SAS-pc based on WARD and average approaches. However, method of average linkage between groups under UPGMA (Unweighted Pair-Group Method using Arithmetic average) was considered as major and final protocol for data conclusion and the resulting clusters were expressed as dendrograms. Various methods generated similar dendograms. This study reveals the phylogenetic relationship of peanut cocoon strains of Iran germplasm. Based on data from studied characters, we constructed dendrograms that resolved the 51 silkworm strains into 2 major clusters. However, the strains of the same origin did not grouped together, demonstrating they can have different biological and development performance. First cluster divided into one sub-group included five strains. However, second cluster divided into two sub-groups. Other strains were grouped together and far from other silkworm strains, indicating they might be suitable for future crossings, maintenance of parental strains and hybridizations with oval cocoon strains so as to maximize heterosis and to avoid depression inbreeding.
  A. Shabdini , A.R. Seidavi , S. Gharahveysi and S.Z. Mirhosseini
  Considering the lake of sufficient information in connection with the three commercial line of silkworm 32, 104, 110 of Islamic Republic of Iran, the objective of research is to investigate the effect of sex, generation, line on economic cocoon traits in these 3 commercial lines. Economic cocoon traits of 3 lines 32, 104, 110 by utilizing 11259 records obtain from four generation in Iran Silkworm Research Center (ISRC) was assessed. The fixed effect of sex, generation, line on traits studied were significant (p<0.01). The commercial lines regarding cocoon traits were also significant which demonstrated the various genetic diversities of commercial lines of silkworm. The total average of the cocoon weight was measured at 1.667±0.0045. The averages of Cocoon Weight trait (CW) in line 32, 104, 110 were 1.734±0.0084, 1.6997±0.0047 and 1.5654±0.0066, respectively. The effect of line was very significant (p<0.001). The effect of the generation was significant too (p<0.001) and the average of 1st generation was higher than other generations and after that there were 3rd, 2nd and 4th generations. The effect of sex was very significant and the average of CW in females and males were 1.85±0.005 and 1.48±0.003 g, respectively. Total mean of Cocoon Shell Weight (CSW) was 0.3618±0.0009 and average of this trait in line 32, 104 and 110 were 0.3902±0.0016, 0.3675±0.0013 and 0.3268±0.0019 g. Effect of all fixed effects were significant on cocoon shell weight. Total average of Cocoon Shell Percentage (CSP) was 21.89±0.044. Average of this trait in line 32, 104 and 110 were 22.736±0.789, 21.8416±0.752 and 21.0677±0.694%. All of the fixed effects were significant on this trait. Average of 1st generation for this trait was 23.794±0.184 and the average of 2nd, 3rd and 4th generations were measured at 21.723±0.079, 21.936±0.081 and 21.628±0.072, respectively. In addition the value of this trait was higher in male than female.
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