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Articles by S.P. Geetha
Total Records ( 3 ) for S.P. Geetha
  A.V. Raghu , Gerald Martin , V. Priya , S.P. Geetha and Indira Balachandran
  The study of cost reduction alternatives in micropropagation of Centella asiatica was reported. Low cost options adopted in the study were effective in lowering cost of production without compromising the quality of plants. The cost on media ingredients was reduced by using household sugar instead of laboratory grade sucrose and tap water instead of double distilled water. Use of liquid media during multiplication stage also helped in reducing the cost on agar. Rooting of in vitro developed shoots under ex vitro conditions also helped cost effectiveness.
  Satheesh George , S.P. Geetha and Indira Balachandran
  A protocol has been standardized for mass in vitro propagation of Baliospermum montanum. Among the cytokinins, BA was superior to kinetin in terms of the number of shoots produced per explant. All the media containing cytokinins and auxins in combination showed superior results than the media with cytokinins alone. Murashige and Skoog (MS) medium containing 1.0 mg L1 BA in combination with 0.1 mg L1 NAA was superior for shoot multiplication. In this medium an optimum number of 9.25 shoots per explant was produced after six weeks of incubation. Among the auxins tried for root induction, NAA and IAA were the most and least effective in root induction, respectively. MS medium containing 0.2 mg L1 NAA was superior for root induction with an average number of 6 roots per shoot. Rooted plantlets on transfer to moist sand, got established successfully in one week and were planted out to pots containing sand, soil and cow dung in 1:1:1 ratio. The micropropagated plants were successfully established with 90 percent success.
  A.V. Raghu , S.P. Geetha , Gerald Martin , Indira Balachandran , P.N. Ravindran and K.V. Mohanan
  An efficient and rapid in vitro clonal propagation of the endangered medicinal tree Aegle marmelos (L.) Corr. (Rutaceae) by enhanced axillary shoot proliferation from mature single node was designed. The explants showed marked seasonal variation in their response under in vitro conditions. Explants collected in October (72.8%) and November (78.6%) showed maximum response. Multiple shoots were formed on Murashige and Skoog (MS) medium supplemented with 0.5 mg L-1 6-Benzyladenine (BA). An average of 6.2 shoots/explant could be obtained after 45 days of culture. The number of shoots was increased at the third subculture with an average of 16.3 shoots per explant. The effect of subsequent subcultures (upto 20 cycles) on shoot formation was also studied. Subculturing was carried out every 45 days on fresh shoot multiplication medium. Continuous culture in the same medium resulted in distorted and vitrified shoots. Transfer of cultures to half strength MS medium devoid of ammonium ions and cytokinin (BA) for a single cycle before going to the shoot multiplication medium could solve this problem. In vitro rooting was inconsistent in medium with different auxins (Indole 3-butyric acid-IBA, Indole 3-acetic acid-IAA and α-naphthalene acetic acid-NAA) at varying concentration and combinations. But in vitro raised shoots could be rooted ex vitro by pulse treatment with naphthoxy acetic acid (NOA) and IBA and then in chlorogenic acid followed by planting in moist sand. This treatment resulted in 83.9% survival of plantlets. The method standardised could be used for large scale planting material production and conservation of this important endangered medicinal tree.
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