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Articles by S.K. Gupta
Total Records ( 5 ) for S.K. Gupta
  P. Thomas , T.R. Arun , K. Karthik , P.V. Berin , M. Asok Kumar , Neetu Singh , J. Usharani , M. Palanivelu , S.K. Gupta , K. Dhama and K.N. Viswas
  Necrotic enteritis, caused by Clostridium perfringens, is an important bacterial disease of poultry. A suspected case of necrotic enteritis was presented for necropsy from an Indian Kadaknath Fowl flock showing diarrhea and progressive debility. Gross examination revealed necrotic to ulcerative lesions in intestine. The organism was isolated from the intestinal contents, tissue and liver under anerobic conditions. The cultural characteristics and Gram staining were suggestive of C. perfringens. The sequencing of 16s rRNA gene confirmed the isolate as C. perfringens and which was well differentiated from other clostridia associated with avian intestinal tract. This study demonstrates that 16s rRNA gene sequencing can provide rapid and confirmatory identification of C. perfringens. Further, Multiplex Polymerase Chain Reaction (mPCR) was performed for toxinotyping and isolate was found to be positive for α toxin (cpa) and β2 toxin (cpb2), a feature of C. perfringens type A isolates. As some recent studies have highlighted the involvement of NetB toxin in pathogenesis, therefore, PCR was carried out to find the presence of this toxin, the isolate was found to be negative for netB gene. This study emphasizes the molecular characterization and toxinotyping as a rapid tool for detection of C. perfringens from suspected necrotic enteritis cases. Very few reports regarding molecular characterization are available from India, hence it adds to the available data on this important poultry pathogen. Further investigations are required to understand the exact role of NetB toxin in pathogenesis as various studies including the current one reports NetB negative strains involved in necrotic enteritis.
  Deepak Sumbria , L.D. Singla and S.K. Gupta
  The veterinary, medical and economic importance of arthropods across the globe and the historical status of some important arthropod borne diseases have been reviewed. Due to hasty altering thermo-climatic conditions in different parts of the world, a variety of medical and veterinary related arthropod borne diseases are expected to rise. Arthropods can infect various parts of living vertebrates, which in turn feed on the host’s tissues and body fluids, often causing extensive damage left untreated. The feeding success of arthropods is linked to the vast array of pharmacological substances in their saliva, which interfere with the host haemostasis and immune response. Reducing arthropod abundance is an imperative but elusive ambition. Some arthropods transmit pathogens that affect humans and animals worldwide. Chemical pesticides applied to territory occupied by these harmful vectors can be valuable but appear to have significant negative effects on other non-target beneficial organisms. Thus, vaccination and biological control need to be explored. Natural biological control is affected by native or co-evolved natural enemies in the environment without human intervention. The purpose of this review is to synthesize the available information concerning arthropod vectors and vector-borne diseases, mainly of public health significance. In conclusion, a low number of investigations on various aspects of arthropod borne infections as well as incuriosity to report the cases in disease reporting system have made the arthropod borne diseases as a more or less neglected field.
  Yogendra Arya , Narendra Kumar , S.K. Gupta and Pankaj Chawla
  This study presents the Load Frequency Control (LFC) of four-area interconnected reheat thermal power system using Fuzzy Logic based PI controller (FLPI). The system is incorporated with governor dead band, generation rate constraint non-linearities and boiler dynamics. The conventional PI controller does not yield adequate control performance when considering the non-linearities and boiler dynamics. The aim of FLPI controller is to restore the frequency and tie line power very smoothly to its nominal value in the shortest possible time. Four performance criteria, i.e., settling time, peak overshoot, Integral Absolute Error (IAE) and Integral of Time Multiplied Absolute Error (ITAE) are utilized for the comparison. The comparison between the conventional PI controller and the proposed controller show that the proposed controller can generate the best dynamic response following load perturbation in either area of the system. Robustness of the proposed controller is achieved by analyzing the system responses with varying system parameters.
  K.P. Kiran Kumar , A.K. Sinha , S.K. Gupta , K.N. Madhusudhan and B.C. Prasad
  Pebrine disease in tasar silkworm is considered to be most serious because of its chronic pathogenicity. Prophylactic method of mother moth examination proves to be the foolproof technique for pebrine identification. So, the study was conducted to investigate pebrine spores detection competency and evaluation of their quality in exhisting mother moth examinations. Four existing mother moth examination methods namely Prick and See, Conventional, Fuziwara and Delayed Mother Moth Examination (DMME) by Fuziwara were selected for the study. The maximum pebrine and hatching percentages were noticed in 4 days DMME with 23.2 and 93.1%, respectively. While, minimum values were observed in prick and see with 4.3% and 84.3, respectively. No significant variations in Cocoon weight, Shell weight and S.R% was observed in cocoons obtained from the different mother moth testing methods. Least percentage of effective rate of rearing (ERR) was observed in Prick and See method (58.75%) followed by Conventional method (61.25%) and Fuziwara (63.15%). High percentage of ERR was observed in DMME which ranged from 64.53% (1st day after egg laying) to 67.28% (4th day after egg laying). Least percentage of improvement of ERR% over Prick and See was observed in Conventional (2.50%) followed by Fuziwara (4.40%). High percentage of ERR over Prick and See was observed in DMME which ranged from 5.78% (1st day) to 8.53% (4th day). Consequently, among the tested mother moth examinations, DMME by Fuziwara after delaying four days from the egg laying has given best results. This method can be implemented in the research institutions for the maintenance of germplasm and breeders stock.
  Shashwati Sen , Madhvi Sharma , Vivek Kumar , K.P. Muthe , P.V. Satyam , Umananda M. Bhatta , M. Roy , N.K. Gaur , S.K. Gupta and J.V. Yakhmi
  Tellurium nanotubes have been grown by physical vapor deposition under inert environment at atmospheric pressure as well as under vacuum conditions. Different techniques such as scanning electron microscopy (SEM), transmission electron microscopy (TEM), X-ray diffraction (XRD) and optical absorption have been utilized for characterization of grown structures. Films prepared using both types of tellurium nanotubes were characterized for sensitivity to oxidizing and reducing gases and it was found that the relative response to gases depends on the microstructure. Nanotubes prepared at atmospheric pressure (of argon) showed high sensitivity and better selectivity to chlorine gas. Impedance spectroscopy studies showed that the response to chlorine is mainly contributed by grain boundaries and is therefore enhanced for nanotubes prepared under argon atmosphere.
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