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Articles by S.H. Kua
Total Records ( 2 ) for S.H. Kua
  P. Hanachi , S.H. Kua , R. Asmah , G. Motalleb and O. Fauziah
  Normal biochemical processes in human body may produce free radicals. These free radicals can, in turn, lead to oxidative stress related disease. This study examined the antioxidant activity of Berberis vulgaris fruit extract and its cytotoxic effect on human liver cancer cell line (HepG2).The antioxidant activity of Berberis vulgaris Fruit Extract (BFE) was assay by β-carotene bleaching and 1-diphenyl-2-picrylhydrazyl (DPPH) assay. The screening of cytotoxic effect was carried out by the microculture tetrazolium salt (MTT) assay on the human liver cancer cell line (HepG2). The BFE with concentration of 5-140 μg mL-1 was used. The control group cell was without any treatment. Intracellular Alkaline Phosphatase (ALP) activity is determined by p-nitrophenyl phosphate. The concentration of 5 μg mL-1 was chosen for this test. In β-carotene bleaching, ascorbic acid showed the mean total antioxidant activity of 96.16±5.09%, f ollowed by BHT (66.71±2.52) and BFE (59.91±8.64). In DPPH, the EC50 of ascorbic acid was 0.252±0.000 mg mL-1, BHT (0.612±0.009 mg mL-1) and BFE (0.685±0.033 mg mL-1). The IC50 of BFE was found 106.0±10.1 μg mL-1. Beside reduction in cell proliferation the crude extract was capable of enhacing the intracellular protein content in cell cancer line by one fourth while intracellular alkaline phosphatase activity increased by 7 fold. The results showed that processed commercial Berberis vulgaris exhibited antioxidant properties, has the ability of reducing cell viability and may had the potential of enhancing the ALP activity probably through structural changes.
  G. Motalleb , P. Hanachi , S.H. Kua , O. Fauziah and R. Asmah
  This study was carried out to determine the total antioxidant activity and phenolic content of barberry (Berberis vulgaris) fruit extracts. The extract was prepared with distilled water and ethanol and methanol (80%), respectively. The -carotene bleaching and 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay were used to determine antioxidant properties of barberry fruit by measuring the decrease in absorbance at 470 and 517 nm. In distilled water, Berberis vulgaris showed 82.52±0.64% free radical scavenging activity. In ethanol, Berberis vulgaris fruit extract showed 73.62±1.87% free radical scavenging activity, BHT 67.50±0.53% and vitamin C 88.56±0.43%, respectively. Berberis vulgaris fruit extract exhibited the higher free radical scavenging activity in distilled water with 82.52% and EC50 = 0.64 mg mL 1. The methanol 80% extract of Berberis vulgaris showed 60.15% -carotene bleaching assay and vitamin C 91.17%. Berberis vulgaris fruit extract in 80% methanol had the highest phenolic content followed by extract in water. The results of showed significant differences (p<0.05) in the means of free radical scavenging activities of barberry fruit in water and ethanol.

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