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Articles by S. Mondal
Total Records ( 3 ) for S. Mondal
  G. Halder , S. Mondal , S.K. Paul , B. Roy and G. Samanta
  An experiment was conducted to find out the effect of arsenic with or without excess supplementation of methionine and methionine-betaine combination on performance of layer chicken. Sixteen week old pullets (n = 120) of Rhode Island Red (RIR) were randomly assigned into four dietary treatments replicated three times with 10 birds/replicate. The groups were control (C)-provided basal diet to meet all the nutrients requirement, T1-birds were offered basal diet and 5.5 ppm arsenic (As) through water, T2-birds were offered control diet with 50 g methionine per 100 kg of feed and 5.5 ppm As through water and in T3- 25 g methionine + 25 g betaine per 100 kg of control feed and 5.5 ppm As through water were provided. The birds were maintained replicate wise in deep litter system of housing with a common system of management. Final body weight and body weight gain was significantly (p<0.01) varied among the groups. Reduced feed intake was noticed in arsenic treated groups. Egg production and egg weight were significantly (p<0.01) decreased in arsenic treated groups than control. The first laying age were differed significantly (p<0.01) among the experimental groups.
  S.K. Tripathi , S. Nandi , P.S.P. Gupta and S. Mondal
  The present research was undertaken to study the effect of a saturated fatty acid-Palmitic Acid (PA), an unsaturated fatty acid-Oleic Acid (OA) and also to investigate the efficacy of Oleic acid on prevention of detrimental effect of saturated fatty acids on oocyte development in ewes (Ovis aries). Sheep oocytes were matured in vitro in the presence of different concentration of palmitic acid (0, 20, 40 and 60 μM) in experiment 1 and oleic acid (0, 40, 60 and 80 μM) in experiment 2 for 24 h. Oocytes were then in vitro inseminated and evaluated for cleavage rates after 42 h post insemination and presumptive zygotes were evaluated for morulae/blastocyst stages after 7-8 days. In experiment 3, oocyte were matured in vitro in presence of, (a) Palmitic acid (60 μM)+oleic acid (40 μM) in oocyte maturation medium and (b) Oleic acid (40 μM)+stearic acid (30 μM) in medium for 24 h. Palmitic acid, was found to impair the maturation, viability, cleavage and embryo production rates at the level 60 μM in ewes. In contrast, oleic acid improved the oocyte development in vitro. Oleic acid also compensated for the unfavorable effects of palmitic and stearic acid on oocyte development.
  I.J. Reddy , Ashish Mishra , S. Mondal and H.N.N. Murthy
  Prolactin (PRL) is a peptide hormone secreted by anterior pituitary gland. PRL increases as per the age and reproductive cycle of birds from normal physiological levels to extremely higher level there by affecting the ovulation, egg formation, oviposition, egg production and hyperprolactinemia. This is more pronounced and persistent after 72 weeks of age in birds. Hence a study was conducted in anterior pituitary primary cultured cells obtained from 72 and 82 weeks old white leg horn (WLH) hens to knock down the PRL gene expression by siRNA and observing its effects on PRL, PRL mRNA, protein content of PRL, prolactin receptor (PRLR) and Growth Hormone (GH) mRNA to unravel the functional role of PRL at 72 and 82 week age by siRNA for PRL. Three siRNAs were designed as per standard siRNA protocols and studied the suppression of PRL gene expression in primary cultured cells procured from adult chicken anterior pituitary glands in in vitro conditions. Average percentage reduction of PRL in anterior pituitary primary cell culture following siRNA transfection was 82 and 60% at 72 and 82 weeks respectively. Protein content of PRL was significantly (p<0.01) decreased in siRNA transfected cells compared to controls. Growth Hormone (GH) mRNA and PRL receptor (PRLR) mRNA levels did not change significantly (p<0.01) between control and treated cells. Results clearly suggested that the siRNA designed for PRL specifically decreased PRL gene expression in in vitro conditions. Level of PRLR mRNA and GH mRNA levels expression did not follow the similar pattern of PRL gene expression in anterior pituitary cells. It is concluded that, construction of short specific siRNA for PRL significantly decreased PRL, PRL mRNA and protein content of PRL without showing any effect on PRLR and GH between the two age groups of birds. These results may lead to construction of short specific siRNA for stable and chronic suppression of PRL gene expression during embryogenesis before an increase of PRL occurs for long term knock down of PRL in in vivo conditions. In conclusion, understanding of hyperprolactinemia and the involvement of PRL may provide the basis for the development of therapeutic drugs or methods against hyperprolactinemia by RNA interference.
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