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Articles by S. Das
Total Records ( 5 ) for S. Das
  D. Biswas , S. Das , B.C. Das and A.K.M. Saifuddin
  A 9-year-old virgin German shepherd was admitted with continuous sanguinopurulent vaginal discharge, distended abdomen, and cachexia. The dog was clinically diagnosed with pyometra and successfully cured by ovario-hysterectomy. The results of histo-pathological examination showed that the endometrial glands were larger than normal and that part of the endometrial wall had sloughed off and become infiltrated with mostly neutrophils. This is the first case report of endometritis and cystic endometrial hyperplasia in a virgin German shepherd in Bangladesh.
  B.C. Saha , P.M. Das and S. Das
  A zinc-selenium complex (Selcon®) was evaluated for the prevention of IBDV infection in broilers. Chicks were reared in relative isolation with optimum temperature, water and feeds. They were divided into 4 different groups named A1, B1, A2 and B2; having 3 replications each. Among them Groups A1 and B1 were uninfected group of which Group B1 was supplemented with Selcon®. The rest 2 groups (A2 and B2) were Infected (challenge) group of which Group B2 were supplemented with Selcon®as a preventive measure. At 28 days of age all the birds of challenge Groups (A2 and B2) were inoculated with field homogenates of Infectious Bursal Disease (IBD) virus. The birds were observed for clinical signs, morbidity and mortality at every 6 h interval upto 10 days post infection. Three birds from each of the four groups of three replications were sacrificed at 0, 3 and 6 days post infection and bursa/body weight ratio was determined. Part of bursal tissues were fixed in formalin and processed for bursal lesion scoring along with spleen, thymus, liver and kidney tissues by histopathological study. Selcon® treated IBDV challenged birds of Group B2 revealed 44.44% overall cumulative morbidity and 2.22% overall cumulative mortality, while Selcon® untreated IBDV challenged birds of Group A2 revealed 60.00% overall cumulative morbidity and 11.11% overall cumulative mortality. Results also showed significant difference (p>0.05) in bursa/body weight ratio between Selcon® treated and untreated groups, that overall bursa/body weight ratio in birds of Group B2 was highest (1.30"0.44) and lowest (1.23"0.42) in those of Group A2 had at 6 days post infection. The bursal lesion scores of Group B2 were lower than Group A2. There was no significant variation among the histopathological lesions of spleen, thymus, liver and kidneys of different groups. It may be concluded that supplementation of Selcon® to broiler diets as a preventive measure provides a satisfactory level of protection against morbidity and mortality in IBD.
  A.B.M. Shahinuzzaman , A.K. Saha , A.C. Mazumder , S. Das , M.A. Sufian , M.A. Baki and M.M. Hossain
  The research work was conducted to study the sequential pathological changes of Pullorum Disease (PD) and immunohistochemical detection of its causal agent (Salmonella pullorum) in various tissues of experimentally infected chicks. Total 40 day old (D0) chicks were divided into experimental and control groups. The chicks were infected at day 15 (D15) of age by oral route with 1 ml of 2 x 108 CFU of S. pullorum. Chicks were sacrificed at day 1 (D1), day 3 (D3), day 5 (D5), day 7 (D7) and day 9 (D9) of Post Infection (PI) and observed the remarkable gross lesions in liver, lung, heart and cecum. Grossly, liver found fragile (40%) at D7 and D9. Cheesy materials in cecum (20%) showed at D9. The highest reisolation of S. pullorum demonstrated in cecum (68%). Histopathologically, nodular lesion in liver developed at D7 (20%) and D9 (40%). Hepatitis from D1 (20%) and continued upto D9 (60%). Pneumonia and bronchopneumonia along with inflammatory cells in lung were observed at D1 (20%) that continued upto D9 (80%). Spleen showed depletion of lymphocytes at D7 (40%) and D9 (60%). Typhlitis in cecum noticed at D5 (20%) and remained up to at D9 (40%). Congestion and hemorrhage was common in organs at early times and gradually reduced both in grossly and histopathologically thereafter. Immunohistochemistry revealed S. pullorum bacteria in the cytoplasm of hepatocytes of liver, in the cytoplasm of epithelia of cecum (D3, D7) and in the cytoplasm of epithelia of crop (D3).
  S.K. Sinha , S. Das , R.C. Misra and B.R. Singh
  Not Available
  S. Das , M. R. Pingle , J. Munoz-Jordan , M. S. Rundell , S. Rondini , K. Granger , G.- J. J. Chang , E. Kelly , E. G. Spier , D. Larone , E. Spitzer , F. Barany and L. M. Golightly
  The detection and successful typing of dengue virus (DENV) from patients with suspected dengue fever is important both for the diagnosis of the disease and for the implementation of epidemiologic control measures. A technique for the multiplex detection and typing of DENV serotypes 1 to 4 (DENV-1 to DENV-4) from clinical samples by PCR-ligase detection reaction (LDR) has been developed. A serotype-specific PCR amplifies the regions of genes C and E simultaneously. The two amplicons are targeted in a multiplex LDR, and the resultant fluorescently labeled ligation products are detected on a universal array. The assay was optimized using 38 DENV strains and was evaluated with 350 archived acute-phase serum samples. The sensitivity of the assay was 98.7%, and its specificity was 98.4%, relative to the results of real-time PCR. The detection threshold was 0.017 PFU for DENV-1, 0.004 PFU for DENV-2, 0.8 PFU for DENV-3, and 0.7 PFU for DENV-4. The assay is specific; it does not cross-react with the other flaviviruses tested (West Nile virus, St. Louis encephalitis virus, Japanese encephalitis virus, Kunjin virus, Murray Valley virus, Powassan virus, and yellow fever virus). All but 1 of 26 genotypic variants of DENV serotypes in a global DENV panel from different geographic regions were successfully identified. The PCR-LDR assay is a rapid, sensitive, specific, and high-throughput technique for the simultaneous detection of all four serotypes of DENV.
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