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Articles by S. Chowdhury
Total Records ( 2 ) for S. Chowdhury
  M.I. Hoq , M.U. Seraj and S. Chowdhury
  Both induced and non-induced hemolymphs were collected from the mud-crab, Scylla serrata and were subjected to antibacterial assay to investigate the presence of inducible and constitutive antibacterial protein(s). Induction was done by injecting Escherichia coli ATCC 25922. The induced hemolymph showed antibacterial activity against a range of different strains of marine or non-marine gram-positive and gram-negative bacteria including few antibiotic resistant strains, whereas the non-induced hemolymph was not appeared to be active against those tested. The induced hemolymph was subjected to SDS-PAGE and seven proteins were detected. The molecular weight of which were in the range from 36 to 84.5 kDa. The hemolymph proteins were fractionated by gel filtration chromatography. Repeated antibacterial assay of the chromatographic fractions against previously proved hemolymph sensitive bacteria showed that only a few fractions were responsible for the activity and that the fraction purified proteins showed better activity than the crude hemolymph. Total five different proteins were present in all the fractions detected by SDS-PAGE analysis. The molecular weights were 64 kDa, 61 kDa, 56.5 kDa, 49 kDa and 36 kDa.
  P.K. Biswas , B.K. Sil , R. Faruque , S. Ahmed , D. Biswas and S. Chowdhury
  A disease outbreak was investigated in a commercial broiler parent stock farm. The outbreak began at the mid of May 2000 and continued more than one month. During the period mortality was recorded around 4 % in the affected shed, flock strength of which was 5000 birds. During post mortem examination the pericardial sac was found engorged with straw colour pericardial fluid. Numerous subcapsular petechial and ecchymotic haemorrhages were seen in liver. No bacterial growth was found after 48 hours of incubation on Muller-Hinton and MacConkey agar surfaces inoculated from the pathological sample. But poor viral growth was recorded in chick embryo fibroblast cell culture propagated inoculums making from heart and liver. Harvested virus using as antigen source from the growth was found reacted positively in agar gel precipitation test with reference conventional adenovirus polyvalent antiserum. Two of the four randomly collected serum samples of the affected flock at day 20 of the outbreak were also found reacting positively with the known reference antigen produced by the same institute. Not having the type specific antiserum the present study failed to elucidate the stereotype (s) that involved in the said outbreak. Nevertheless, the study seems to be the first report of adenoviral induced hydropericardium-hepatitis syndrome or leechi heart disease affecting chicken in Bangladesh.
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