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Articles by Ruchi Saumya
Total Records ( 4 ) for Ruchi Saumya
  N. Rajendra Goud , Ujjwal Neogi and Ruchi Saumya
  The main objectives of this study was to isolate and screening of the fungi for the production of GLA which can be further used to large industrial scale production of GLA from microbial sources. Gamma-linolenic acid, (GLA; cis-6, cis-9, cis-12-octadecatrienoic acid) is an omega-6 fatty acid found in Oenothera biennis, Borago officinalis, Ribes nigrum and fungi mainly in the division Phycomycetes. GLA is extensively used in diabetes, Sjögren's syndrome, osteoporosis, menopausal symptoms, premenstrual syndrome (PMS), allergic rhinitis, rheumatoid arthritis, attention deficit/hyperactivity disorder (ADHD), alcoholism, cancer, weight loss, high blood pressure, heart disease and ulcers. In this study, lipid extraction was carried out by Soxhlet extraction method using petroleum ether (60-80°C). It was found out that the total lipid contents of Cunniinghamella I is more (21.65%) with compared to Mucor II (19.20%) but the GLA content was more (06.34%) in Mucor II. Rhizopus I, Cunniinghamella III, Absidia II, Mucor I strains also had more lipid contents. Stearic acid and oleic acid content was more in all the cases (40-70%). Further the study was carried out using different carbon and nitrogen sources and different physical conditions like different pH, aeration etc to determine the highest yielding conditions with Cunniinghamella I, Rhizopus I, Absidia II and Mucor II. It was found that pH, aeration and C:N ratio played very important role in growth of organism, biomass production and GLA production. In 30:3 C:N ratio, glucose was best carbon source than sucrose because glucose in synthetic media enhances the biomass and the oil contents with compared to 30:6 C:N ratio where the biomass production was more but the oil content was less. In pH 5 the GLA production was more compared to pH 3 and 9. Results of these kind heralds an interesting promise for industrial scale production of GLA from the Phycomycetes.
  K.C. Raju , Ujjwal Neogi , Ruchi Saumya and N. Rajendra Goud
  Keratinases (E.C. are of particular interest because of their action on insoluble keratin substrates and generally on a broad range of protein substrates. The objective of this study is to isolation, screening, purification and determination of the enzymatic activity of extracellular keratinase from dermatophyte Microsporum gypseum. The study clearly indicates the presence of the enzyme keratinases in the dermatophyte Microsporum gypseum. One milliliter of the purified sample contain 80 μg of protein, 1.09 μmole mL-1 60 min enzyme activity and 13.6 μ mole mg-1 60 min specific activity with respect to the unpurified one. The purified and unpurified state of the enzyme was judged by SDS/PAGE. Purified enzyme showed a single band of molecular weight of 33 kDa. Characterization studies showed optimum activity at pH 8 and at 35°C. The enzyme kinetics increased with increased concentration of MgCl2 and decreasedwith increased concentration of ZnCl2. Maximum biomass and keratinase activity were observed from pH 7.0 to 9.0, which agrees with those described for most feather-degrading Bacillus. In this study, the optimum conditions for keratinase synthesis by the Microsporum gypseum were determined, which will be an essential step for the production of adequate amounts for application in research field and other areas.
  Ujjwal Neogi , Ruchi Saumya and Bushra Irum
  The antibacterial effects in vitro of crude ethanol and aqueous extracts of Garlic (Allium sativum), Ginger (Zingiber officinale), Turmeric (Curcuma longa) and Neem (Azadirachta indica) were assayed against Staphylococcus aureus, Salmonella typhi and E. coli were positive. The antibacterial activity was determined only by agar disc diffusion method and it gave clear zone of inhibition. The aqueous and ethanolic plant extracts containing diffusion disc of diameter 5 mm with 167 μg/disc, gave the zone of inhibition. The zone diameter was less in single extracts but in combinations it was more effective. The highest inhibition zone of 15 mm was observed with synergistic combinations of Garlic and Turmeric (70% Ethanolic) extracts on Staphylococcus aureus, Garlic and Turmeric (Aqueous), Garlic and Turmeric (70% Ethanolic), Ginger and Turmeric (70% Ethanolic) on Salmonella typhi. The E. coli was more resistant than the other two organisms. Highest zone of inhibition observed with Garlic and Turmeric (Aqueous), was 13 mm. Results of these kind herald an interesting promise of designing a potentially active antibacterial synergized agent of plant origin. The control experiment was done with O-Trimoxazole, Ampicillin-A, Cloxacilin, Chloramphenicol of Hi-media Company.
  G. Kesava Naidu , S.M. Gaddad , C.T. Shivannavar , N. Rajendra Goud , Ujjwal Neogi and Ruchi Saumya
  The main objectives of this research was to isolate Shiga Toxin Producing Escherichia coli (STEC) from diarrheal samples, stool samples from cattle, beef, mutton samples, water and sewage samples collected from different places in Gulbarga region of Karnataka, India and determination of the antibiotic susceptibility patterns of STEC. The highest number of STEC was found in the Sewage sample (14.84%) where as urine sample did not contain any STEC. Among the 2109 sample 65 were confirmed as STEC. The highest (73%) incidence of resistance was recorded against Ampicillin, closely followed by that against streptomycin (70.77%) and cephalexin (69.23%).While only two antibiotics, chloramphenicol (21.54%) and gentamicin (12.3%) recorded comparatively lower incidence of resistance. The multiple antibiotic resistances were most common. More than 98% of the isolates were resistant to two or more antibiotics. Resistances to three (11 isolates), six (10 isolates) and five (9 isolates) antibiotics were most common. Alarming to note that, few isolates (4.61%) are resistant to all the 12 antibiotics tested and 21.5% of the isolates are resistant to ten or more antibiotics.
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