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Articles by Roghayeh Ghorbanzadeh
Total Records ( 3 ) for Roghayeh Ghorbanzadeh
  Abbas Bahador , Hormozdyar Etemadi , Bahram Kazemi , Mahbobeh hajabdolbaghi , Roghayeh Ghorbanzadeh and Omid Pajand
  The objective of this study was to compare characteristics of Direct and Concentrated Flurochrome (auramine-rhodamine) smears for the detection of Mycobacterium sp. and the direct microscopically examination, the concentration method and culture. A total of 900 sputum specimens from patients diagnosed with pulmonary tuberculosis were tested for the presence of mycobacteria. In this study we used the direct staining and an improved technique after treating the smears with N-acetyl-L-cysteine, NaOH and sodium citrate and concentration of bacteria by centrifugation. Specimens were stained using the auramine-rhodamine technique. The gold standard was defined as a positive Löwenstein-Jensen culture, or a positive AFB smear of material obtained from a negative culture after 60 days. Fifty two specimens were positive according to the gold standard definition. Decontamination and concentration of the sample increased the sensitivity of the direct microscopically examination from 71.1 to 88.4%. The concentration method adds significantly to the sensitivity of the direct microscopic examination, without much extra input.
  Abbas Bahador , Hormozdyar Etemadi , Bahram Kazemi and Roghayeh Ghorbanzadeh
  Polymerase Chain Reaction (PCR) has been widely used due to its high specificity, sensitivity and rapid turn-around time. However, inhibitory factors may be co-extracted with the target nucleic acid that will hinder the performance of PCR. The major difficulty with mycobacteria is achieving optimal cell lysis. Due to a genuine need for an accurate test for the diagnosis of tuberculosis a comparison of DNA extraction methods was conducted. DNA extraction methods for Mycobacterium tuberculosis were evaluated including Triton, Chelex, Nonidet, SDS/Lysozyme and Silica-based methods on bacterial cells and spiked sputum. DNA extracted from the above procedures was diluted from 10-1 to 10-7 for use as templates in the PCR titration assay. The PCR end point was at a dilution of 10-2 when DNA was extracted using the Triton and Chelex extraction methods. It was 10-3 for Nonidet and 10-4 for SDS/Lysozyme extraction methods. DNA extractions by silica-based method had PCR end point titrations at the 10-5 dilution. The sensitivity of extraction by silica-based is 1-10 cells. In the present study silica-based method is effective extraction method. The end point titrations are identical for bacterial cells and spiked sputum. Inhibition was not observed in PCR with DNA isolated from spiked sputum. Silica-based method required the least labor and completion time
  Abbas Bahador , Hormozdyar Etemadi , Bahram Kazemi , Mahbobeh Hajabdolbaghi , Mansour Heidari , Abasali Kokab , Roghayeh Ghorbanzadeh , Omid Pajand , Zoya Hojabri and Farzaneh Bazarjani
  Bacille Calmette Guerin (BCG) was initially used as vaccine against tuberculosis, however, it uses for verity of clinical application. Detection of adverse effects and complications of BCG is essential for necessary timely quality control of the vaccine, administration techniques and its application Direct microscopy and culture are still the methods of choice for detection of BCG in most laboratories, but they are very difficult processes and time-consuming. An ESAT-IS1081 multiplex PCR amplification of targeted gene segments was optimized to detect and differentiate the BCG strains from other members of M. tuberculosis complex. The sensitivity of detection for H37Rv, M. bovis and BCG were dilution of 104,105 and 105 cells mL-1 of cell stock suspension, respectively. The results from this study showed that the ESAT-IS1081 multiplex PCR assay permits a specific, sensitive and reproducible system for the detection and differentiation of the BCG, thereby improving the clinical management of BCG complication to clinician.
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