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Articles by Rizkita Rachmi Esyanti
Total Records ( 2 ) for Rizkita Rachmi Esyanti
  Yurnaliza , I Nyoman Pugeg Aryantha , Rizkita Rachmi Esyanti and Agus Susanto
  Ganoderma boninense is a major pathogen in oil palm plantations which easily infects basal stems and thus it is difficult to be eradicated. Fungal endophytes from healthy oil palm tissues can be used as biological control agents in host plants against G. boninense. For selecting the antagonist candidates against G. boninense, a simple method of dual culture assay was used. A total of 526 endophytic fungi were isolated from 531 oil palm segments in three of plantations location in Medan, North Sumatera province of Indonesia i.e. Kuala Bekala (KB), Medan Tuntungan (MT) and Medan Johor (MJ). Overall, fungal isolation rate in oil palm segments was 0.99 and colonization rate was 77.2% and the highest being found in the KB plantation. From different sample parts, we found that palm leaf midrib had the highest value of colonization and isolation rate. Based on six categories in qualitative dual culture assay, 53 isolates showed a high antagonism effect to G. boninense, twenty two of which were found to have a Colony Growth Inhibition (% CGI) value of more than 80%. Further studies showed that all 22 isolates exhibited a chitinolytic activity on Coloidal Chitin Bromcresol purple (CCBp) assay while only ten (crude extract of liquid culture) inhibited mycelial colony of G. boninense. From microscopic observation, fungal endophytes attached themselves to the hyphae of G. boninense at the interaction zone and causing hypae abnormalities to the pathogen. Almost all selected fungal endophytes potential to be biocontrol agents against G. boninense belong to the Genera of Trichoderma.
  Mustika Dewi , Rizkita Rachmi Esyanti and I. Nyoman Pugeg Aryantha
  Background and Objective: Ectomycorrhyza is associated with most trees, including the pine tree. The interaction between ectomycorhizzal fungi with pine trees has been reported in some species from other regions but none from Indonesia, especially in Bandung (West Java). This study aims to determine the diversity of ectomycorrhizal fungi found under pine (Pinus merkusii) stands at various locations in the Bandung area. Material and Methods: The method used in this study includes exploration, collection of the fungal fruiting body, isolation and identification using morphological and molecular approaches. The exploration site was based on the presence of the pine tree in seven locations in Bandung. Results: Seven samples of fruiting bodies representing each location were collected from under stands of pine tree, namely BK-01 (Bojong Koneng), GS-02 (Ganesha campus), TC-03 (Taman Cibeunying), CK-04 (Cikutra), SK-05 (Sukaluyu), CS-06 (Cisitu) and PP-07 (Perum Pahlawan). Conclucion: The identification results based on morphology and the ITS DNA character show that all ectomycorhizal samples belong to the order of Boletales, family of Suillaceae and genera of Suillus. The ectomycorrhizal fungi from Bojong Koneng, Ganesha campus and Perum Pahlawan are identified as Suillus placidus, while the ectomycorrhizal fungi from Taman Cibeunying, Cisitu, Cikutra and Sukaluyu are identified as Suillus granulatus. This study has clearly shown that the diversity of ectomycorrhizal fungi were found under the pine trees in the area of Bandung is a fungi with the name of the species Suillus placidus and Suillus granulatus.
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