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Articles by Rina Ratnasih
Total Records ( 2 ) for Rina Ratnasih
  Dikayani , Sri Nanan B. Widiyanto , Erly Marwani and Rina Ratnasih
  Environment has influenced the growth and development of agricultural crops including banana (Musa acuminata L.). Environmental stresses are caused by biotic factors such as fungi, bacteria and herbivore and abiotic factors such as temperature, water, light and salinity. Salinity stresses cause a decrease in the production of banana, because they affect the growth of the plant in the changing balance of Na+ and Cl‾ ions and changes in the plant’s primary metabolites including fatty acid compounds. The purpose of the research was to identify the fatty acid compounds. The research method was conducted in 2 stages: phase (1) Developing in vitro culture of banana shoots with NaCl treatment of 0, 50, 100, 150 and 200 mM concentrations, (2) Gas Chromatography Mass Spectrometry (GCMS) analysis on the roots of in vitro banana planlets. The results showed that the fatty acid compounds involved in the protection against salt stress. The fatty acid the highest area compound in 0 mM treatment 9 Octadecanoic acid methyl ester were (14:36%), 50 mM treatment 9-octadecanoic acid methyl ester 13.13% and 100 mM treatment Tridecanoic acid 11.8%. In 150 mM treatment 9,12 octadecanoic acid 31.03%, whereas, the highest area in 200 mM treatment 9,12 octadecanoic acid 36.49%.
  Eri Mustari , Diky Setya Diningrat , Rina Ratnasih and Srinanan M. Widiyanto
  Background: Teak (Tectona grandis Linn f.) is one of valuable timber species because of the beauty, strength and durability properties. Main problem in teak improvement program is reproductive biology, a low pollen viability, high embryo abortion, low pollination success and low fruit production. Investigation carried out at the teak improvement that flowering aspects is very important. Teak development program was facing difficulties due to the lack of information about the role of genes regulating flowering. APETALA2 (AP2) and APETALA3 (AP3) genes as member of the floral organ Identity. The role of teak flowering development predicted by up and down regulation of TgAP2 and TgAP3 genes. This study was conducted to determine the gene expression profile of TgAP2 and TgAP3 in the developmental stages of teak flowering. Materials and Methods: In this study, TgAP2 and TgAP3 genes in teak identified from NGS transcriptome data that is annotated with Solanum lycopersicum. The TgAP2 and TgAP3 genes activities on the several developmental stages of teak flowering tissues are done with QRT-PCR analysis using 18S RNA as gene reference. Results: The highest expression level of TgAP2 on the floral development stages of 4th lateral floral buds. The TgAP3 gene expression reached the highest level on the apical floral bud stage development. Vegetative shoot as control stages, TgAP2 gene expression level reached 9 times compared to the control and TgAP3 more than 12 times. The TgAP2 and TgAP3 genes expression profile has non-significant correlation on all floral development stages. Conclusion: The TgAP2 and TgAP3 genes expressed with different level in all stages of the teak flowering development. The TgAP2 and TgAP3 genes have non-significant correlation expression profile in role of teak floral development. These results indicate that the TgAP2 and TgAP3 expression profiles equivalent to the general pattern in the Arabidopsis model plant.
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