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Articles by Renald Blundell
Total Records ( 12 ) for Renald Blundell
  Renald Blundell and David Harrison
  Not Available
  Renald Blundell
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  Renald Blundell
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  Renald Blundell and David Harrison
  Integrins are the primary receptors used by cells to interact with extracellular matrix. Various 1 and 4 integrin complexes were shown to be involved in the activation of a number of cell cyclin inhibitors. The 4 integrin cytoplasmic domain was shown to be involved in the activation of p21 pathways of growth arrest and apoptosis. In this study, the 1 integrin was blocked and cell were cultured on laminin as ECM, thus only the 4 integrins binds to the ECM. This way we were able to study the mouse Clara cell cycle progression and death possible through the regulation of p21 in a controlled integrin-ECM binding environment using both wt and p21-/-mice. Upon beta-1 integrin blocking, an increase in apoptosis was observed in Clara cells from both wt and p21 ko mice at 72 and 120 h in culture, the apoptosis rate was higher (p<0.05) in cells from wt mice compared to cells from p21 ko mice at 72 h and a decrease (p<0.05) in BrdU incorporation was observed in cells from wt mice at 24 and 72 h in culture and in cells from p21 ko mice at 24 h in culture. Cytoplasmic PCNA expression was found to be higher (p<0.05) in cells from both wt and p21 ko mice upon beta-1 integrin blocking at 120 h and the expression of nuclear PCNA expression significantly increased (p<0.05). In the absence of p21, there was an increase in cytoplasmic PCNA expression but not nuclear PCNA expression. The expression of PCNA increases in cells from both wt and p21 ko mice but p21 is essential for the nuclear PCNA localisation.
  Renald Blundell
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  Renald Blundell and David J. Harrison
  The disruption of cell-matrix interactions influences the expression of a number of cell cycle regulatory proteins such as p21, p27 and p53. In this study, Clara cells obtained form both wildtype (wt) and p21 knockout (p21 ko) mice were isolated and cultured on different ECM combinations and the expression of three cyclin kinase inhibitors p21, p27 and p53 were studied. Distruption of the cell-matrix resulted in a decrease in the expression of cytoplasmic p21 and cytoplasmic p27. Nuclear p53 expression was found to increase in Clara cells lacking p21 and although the variation of ECM combinations did not affect the expression of p53. Although the p27 expression did not significantly changes upon culturing the cells in different compositions of ECM, disruption of the cell-matrix interactions seems to be influential.
  Renald Blundell and David J. Harrison
  Cell-matrix interactions or disruption affects the cell cycle regulation in various ways and thus the extracellular Matrix (ECM) can control cell differentiation. In this study, Clara cells obtained form both wildtype (wt) and p21 knockout (p21 ko) mice were isolated and cultured on different ECM combinations. A lower expression of cytokeratins 8, 18 and 19 in unattached Clara cells from p21 knockout (ko) mice compared to wildtype (wt) mice was observed. The expressions of cytokeratin 8 and 19 were significantly higher in primary Clara cells cultures when laminin formed part of the ECM composition. The cell-matrix disruption also played an important part on Clara cells differentiation process.
  Claire Marie Agius and Renald Blundell
  The discovery of stem cells heralded great improvements in the prognosis of a number of patients, in particular those having haematological diseases, the classic example being, of course leukaemia. After these encouraging results, scientists are researching ways of manipulating stem cell technology in order to treat more effectively diseases which decimate affluent societies following the epidemic of obesity, such as cardiovascular diseases for example. Furthermore, recent studies give hope in the fight against autoimmune diseases, as well as sickle-cell anaemia, even going some way towards outlining methods of providing a cure, hopefully in the not-too-distant future.
  Nadia Gamoudi and Renald Blundell
  Stem cells and cancer have been found to be amazingly closely intertwined with one another in such a way that they form a meshwork that can help to explain the genesis of cancer and more importantly lead the way to an effective cure to this dreaded disease. In this study, the link between stem cells and neoplasia is bridged and novel therapies for cancer are reviewed.
  Renald Blundell and David J. Harrison
  Extra Cellular Matrix (ECM) contains signals that control cell progression, division and death. Cell-matrix interactions or disruption affects the cell cycle regulation in various ways. In this study, Clara cells obtained form both wild type (wt) and p21 knockout (p21 ko) mice were isolated and cultured on different ECM combinations. Different ECM compositions did not influence Clara cells proliferation both the in presence or absence of p21. On the other hand, p21 was found to induce both apoptosis and necrosis in primary Clara cells cultures. The cell-matrix disruption rather than ECM composition seems to have a greater influence on the cell cycle regulation and progression.
  Claire Marie Agius and Renald Blundell
  The steady increase in the average life-expectancy in the developed world can be attributed in part at least, to advances in virtually all areas of medicine, starting with technological advances in diagnostic techniques, greater awareness as regards living a healthy lifestyle, more efficient surgery protocols, while less invasive methods are used whenever possible, a greater depth of the etiology and pathogenesis of the disease and so on and so forth. However, despite overcoming a great number of illnesses, other formidable diseases still remain unscathed by even the most aggressive treatment. This seems to be the point in fact, where the promise of stem cells becomes more exciting and poignant. Research investigating the potential use of stem cells against these illnesses yielded a number of encouraging results and even successful therapy protocols in some instances (ex. leukaemia). Some therapies are also in the pipeline while others are still experimental. Having said this, the material available today gives an encouraging boost for all involved in the fight against some of the debilitating and ruthless illnesses of our times.
  Nadia Gamoudi and Renald Blundell
  Neoplasia is a literal translation of the Greek word `new growth`. Neoplastic cells are said to be transformed because they replicate continuously, heedless and in perfect asynchronism to the regulatory influences that govern normal cell growth. As a result, a fundamental characteristic of neoplasms is the loss of responsiveness to regular growth control resulting from failure of the usual mechanisms that control cellular proliferation and maturation. Moreover, this random growth continues even in the absence of the stimulus. In this study, the cellular, molecular and biochemical mechanisms of carcinogenesis are analysed. This entails a detailed discussion of the cell cycle and its fine regulation together with an explanation of the link between the cell cycle and induction of cancer. Proto-oncogenes and tumor-suppressor genes are also discussed. Finally, the genetic aspect of cancer is tackled.
 
 
 
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