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Articles by Rehab A. Dawood
Total Records ( 2 ) for Rehab A. Dawood
  Ahmed M. Soliman , Sabry Y.M. Mahmoud and Rehab A. Dawood
  Garlic samples showing symptoms of Onion Yellow Dwarf Virus (OYDV) were obtained from previous study and tested by indirect-enzyme linked immunosorbent assay (I-ELISA), transmitted to Chenopodium amaranticolor and then confirmed by immunosorbent electron microscopy (ISEM) for the presence of OYDV. PCR primers were used to amplify about 1.1 kb fragment from the viral genome using RT-PCR from infected garlic plants, such fragment were not obtained from healthy- looking plants and/or virus-free seedlings of shoot-tips. The amplified products of OYDV was cloned into pGEM®-T Easy vector and transformed into Escherichia coli strain DH5a. The recombinant plasmids were obtained and sequenced. The nucleotide sequences were compared with corresponding viral nucleotide sequences reported in GenBank. The sequence analysis showed that; nucleotide sequence of OYDV-EG [Egyptian isolate (HM473189)] had 82-96% similarity compared with ten reported OYDV isolates. Thus, a method of identification and detection by RT-PCR of OYDV was established. This study also aimed to obtain OYDV-free plants from infected garlic plants using cloves subjected to electrotherapy, thermotherapy, chemotherapy or meristematic dissection followed by in vitro culture. A combination treatment with electro- and chemotherapy (15 mA/10 min + 20 mg L-1 virazol) was found to more effective on viral elimination and survival of explants. ELISA tests showed that 85% of the plantlets that survived were OYDV-negative.
  Amal A. Ahmed , Eman A.H. Khatab , Rehab A. Dawood and Amira M. Ismeil
  Carnation Latent Virus (CLV) and Carnation Vein Mottle Virus (CarVMV) were of the most important virus affecting carnation plants. The two viruses were detected serologically by DAS-ELISA using specific antibodies. Shoots from plants infected with (CLV and CarVMV) which maintained in green house were used for tip culture. Murashige and Skoog Medium (MS) supplemented with (0.2 mg L-1 BA) and (1 mg L-1 BA and 0.5 mg L-1 kinetin) were used for proliferation and micropropagation of the infected shoot clumps, respectively. The plantlets were rooted in MS medium supplemented with (1.5 mg L-1 NAA). Plantlets regenerated from meristem measuring 0.1 and 0.2 mm yielded (90 and 80%) for CLV and (92 and 80) for CarVMV virus-free tested plantlets respectively, while larger meristems (0.3 and 0.4 mm) were not effective. As well as, the plantlets survival was (20, 35, 65 and 80%) for meristem measuring (0.1, 0.2, 0.3 and 0.4 mm), respectively. Where as the exposure of carnation plantlets infected with CLV and CarVMV to three temperature regimes (36, 38 and 40±°C for 3 and 4 weeks). It was found that (38±1°C/3 weeks) gave (65 and 70%) for CLV and CarVMV virus-tested plantlets, respectively and plantlets survival were (67 and 40%). While (38 and 40±1°C for 4 weeks) gave higher precentage of virus-free plantlets but has a negative correlation with the survival of the plants. However, because of better survival rate, the temperature regime 38±1°C/3 weeks is recomended for virus-free tested carnation plants.
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