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Articles by Rajesh Nigam
Total Records ( 3 ) for Rajesh Nigam
  Lalita Sharma , Amit Kumar Verma , Amit Kumar , Anu Rahat , Neha and Rajesh Nigam
  The main objective of the present study was to report the prevalence of methicillin resistant Staphylococcus aureus (MRSA) among bovines used for milk production in Mathura, India. A total of 80 milk samples were collected from clinical and subclinical cases of mastitis from cows (40) and buffalos (40). Milk samples were processed for isolation and identification of S. aureus using standard bacteriological procedures. Staphylococcus aureus were isolated from only 27 samples showing the overall incidence of Staphylococcus aureus in clinical as well as sub clinical mastitis was 33.75%. The incidence of Staphylococcus aureus was higher (50.00%) in clinical mastitis in comparison to that of subclinical mastitis (17.50%). The results revealed that the incidence of Staphylococcus aureus in clinical as well as sub-clinical mastitis was higher in cattle in comparison to buffaloes. Drug sensitivity revealed the 100% resistance against penicillins followed by vancomycin (88.89%), nalidixic acid (77.78%), cefixime, methicillin, novobiocin (66.67% each), amoxiclav, colistin, pipemidic acid (55.56% each), ofloxacin, streptomycin, sulphamethizole (44.44% each), ampicillin/sulbactam, cefalexin, cefazolin, cefoperazone, enrofloxacin, floxidin, meropenem (33.33% each), cefuroxim, ciprofloxacin, clindamycin, gentamicin, levofloxacin, norfloxacin, tetracycline (22.22% each). Eighteen isolates were found to be methicillin-resistant, while the remaining (09) were methicillin-susceptible. Similarly, twenty four S. aureus isolates were intermediate to vancomycin while three were vancomycin susceptible and no isolate was resistant to vancomycin. Thus, the findings are useful for formulating specific control programs for bovine mastitis caused by S. aureus in this region.
  Vijay Pandey , Rambachan , Rajesh Nigam , Deepak Sharma , Satyendra Pal Singh and Madhu Tiwari
  Background and Objectives: Changes in body condition and fat depots crucially affects the postpartum negative energy balance and succeeding milk production in dairy cows. Thus, this study was planned to elucidate the comparative changes in circulating levels of biochemical profile and leptin hormone during different physiological stages in high and low yielding breeds of Indian dairy cattle. Materials and Methods: Total 6 pregnant Sahiwal and Hariana cows of same age group (approximately 3-5 years) with similar body condition and in their first parity were selected. Blood samples were collected 1 month antepartum (dry) and 1st (early lactation), 2nd (early mid lactation) and 3rd month (late mid lactation) postpartum twice in a month (fortnightly). Plasma was harvested and analyzed for levels of leptin, glucose, urea, triglyceride (TG), cholesterol, High Density Lipoprotein (HDL), Low Density Lipoprotein (LDL) and non-esterified Fatty Acid (NEFA). Results: The significant lower levels of leptin, TG, cholesterol, HDL, LDL and NEFA were observed in high yielding Sahiwal cows compared to low yielding Hariana cows while period of lactation showed significant effect on all the parameters except leptin and NEFA in both the breeds. Interaction of breed and period revealed substantial effect on levels of TG, cholesterol, HDL and LDL. Conclusion: The study suggests their possible role in differential production potential of these high and low yielding animals. Moreover, further studies are warranted to explore the real cause of differential production potential of animals.
  Lalita Sharma , Amit Kumar Verma , Anu Rahal , Amit Kumar and Rajesh Nigam
  Background and Objective: Mastitis causes significant economic losses in dairy industry globally. The present study evaluated levels of Blood Urea Nitrogen (BUN) and lipid peroxidation (LPO) along with enzymic activities of lactate dehydrogenase (LDH), alkaline phosphatase (ALP)and glutathione peroxidase (GPx) in the serum samples, lipid peroxidation (LPO) and activities of glutathione peroxidase (GPx) in skimmedmilk of dairy animals (cows and buffaloes) showing sub-clinical mastitis (SCM) and clinical mastitis. Methodology: A total of 100 lactating animals were divided into two groups i.e., cattle and buffaloes, each group contain 50 animals. Each group is further divided into three subgroups healthy (10), sub-clinical mastitis (20) and clinical mastitis (20). Blood serum and defatted milk were used for enzyme activity estimations. Results: The LDH and ALP activities along with LPO levels were significantly higher (p<0.05) in SCM and CM the milk as compared to healthy milk from udders. Non significant differences were observed in BUN values. The mean activities of GPx were significantly reduced (p<0.05) in SCM and CM milk than in healthy milk. Increased lipid peroxidation in serum and milk indicated direct correlation between oxidative stress and tissue damage in clinical and sub-clinical mastitis in dairy animals. Conclusion: From the present study, it may be suggested that optimum antioxidant intake carry sufficient potential in affording protection against sub-clinical and clinical mastitis in the dairy animals.
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