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Articles by R.P. Singh
Total Records ( 9 ) for R.P. Singh
  Harendra Singh , Pratibha Singh , R.P. Singh and Mritunjay Tripathi
  Health promoting biochemical parameters of 15 distinct chickpea genotypes (Desi and Kabuli) showed wide variability in their chemical composition, crude fiber, total mineral content, total free amino acid Rhizobium inoculated was relatively higher than non inoculated. Reducing sugar, non reducing sugar, total sugar non inoculated was relatively higher than Rhizobium inoculated. Crude fibre content in chickpea ranged from 12.89-13.79% in control, Rhizobium inoculated 13.11-15.03%, total mineral content ranged from 3.55-3.79% in control, Rhizobium inoculated 4.14-4.45%, total free amino acids in chickpea seeds ranged from 2.35-2.51% in control, Rhizobium inoculated 2.38-2.65%, reducing sugar content in chickpea seeds ranged from 24.45-25.80% in control, Rhizobium inoculated 24.05-25.38%, non reducing sugar content in chickpea seeds ranged from 39.06-41.21% in control, Rhizobium inoculated 38.41-40.53%, total sugar content in chickpea seeds ranged from 63.51-67.00% in control, Rhizobium inoculated 62.46-65.91%. The research result about the biochemical characteristics of control and Rhizobium inoculated chickpea genotypes are expected to provide guidelines for the researches confronted with the need to use such typical food seed in India as well as in the rest of the world.
  Pankaj Sharma and R.P. Singh
  The antioxidant capacity of complex heterogeneous foods and biological systems is affected by many factors. Considering the importance of antioxidants, it is of great interest to know the antioxidant capacity of the constituents in foods. The Total Antioxidant Capacity (TAC) is a parameter that provides information on the overall status of antioxidants within a complex biological sample. TAC, as determined by Trolox Equivalent Antioxidant Capacity (TEAC) method, offers several advantages over other methods and is relatively easy. Due to its operational simplicity, the TEAC assay has been used for studying antioxidant capacity and TEAC values of many compounds and food samples. The article deals with various developments in the method, its merits and demerits and its application in determining the TAC of various food commodities.
  Sananda Mondal , R.P. Singh , Chaitali Sen and Bandana Bose
  The regeneration protocol was developed from dehusked and sterilized seeds of rice variety Swarna (MTU 7029) to study callus induction, callus growth rate and regeneration potentiality. Different ranges of 2,4-D (1-4 mg L-1) and combinations of 6-Benzyl Aminopurine (BAP) (2-4 mg L-1) and α-Naphthalene Acetic Acid (NAA) (1 mg L-1) were used for callus induction, somatic embryo formation and plantlets regeneration respectively. Ninty six percent callus induction efficacy was found with 4 mg L-1 2,4-D. Whereas the high frequency of plantlets regenerated from embryogenic calli were observed in the combination of 4 mg L-1 BAP and 1 mg L-1 NAA; the embryogenic calli obtained from 2 mg L-1 2,4-D concentration performed better in this media (4 mg L-1 BAP and 1 mg L-1 NAA) than the other three used concentrations of 2,4-D. Physiological potentiality of 45 days old calli in terms of fresh and dry weights and proline content were showed a positive correlation. However, 4 mg L-1 2,4-D in medium showed low regeneration capacity of calli.
  K. Dhama , R.P. Singh , K. Karthik , S. Chakraborty , R. Tiwari , M.Y. Wani and J. Mohan
  Spallanzani’s thought of Artificial Insemination (AI) has revolutionized the animal husbandry field, both in developing and developed countries, by improving the genetic potential of livestock and poultry; minimizing the managemental costs and holding the service of genetically superior males even after their death. AI in domesticated birds especially in turkey shows promising results unlike other domestic and wild animals. The advantages of AI are many which support the wide adaptation of this technique in the poultry industry to augment its growth. Making AI as an integral part of captive breeding programme for non-domesticated birds would facilitate the process of saving various endangered species of wild birds. However, there are various problems involved in case of birds which need to be addressed before implementing AI. Apart from these, AI also poses a risk of possible transmission of various infectious pathogens/diseases of poultry through semen or its contamination or during the process of insemination. Hence, careful and regular screening and monitoring of poultry will help to check the spread of such diseases. Novel methods are adopted to prevent the colonization of contaminant microbes in stored semen thereby minimizing the pathogen transfer. The recent advances in biotechnology and molecular biology need to be explored fully for early and rapid diagnosis of poultry diseases. This would help in formulating appropriate disease prevention and control strategies and thus safeguard poultry health and production. This review describes the salient facts about AI practices in poultry and possible transmission of infectious pathogens during insemination along with suitable prevention and control strategies to be adapted.
  Harendra Singh , Pratibha Singh , R.P. Singh and Mritunjay Tripathi
  Fifteen genotypes of chickpea control and Rhizobium inoculated were investigated for protein content and genetic divergence based on seed protein profile using Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis (SDS-PAGE). Protein content of desi type was relatively higher than kabuli type. Protein content fifteen genotypes ranged from 18.76-23.58% in control, Rhizobium inoculated 19.83-24.54%. Desi and Kabuli genotype of chickpea Rhizobium inoculated gave some unique band in comparison to control genotype of chickpea. Desi genotypes gave some unique band in comparison to Kabuli genotypes of chickpea. Highest number of protein band found in PUSA 362, KWR 108 and followed by H82-2 and Phule G 5 whereas minimum number of protein bands were found in L550. It is clear from the result obtained that the chickpea genotype having highest number of protein band having highest quantity of protein. Maximum bands are of medium molecular weight followed by highest molecular weight and low molecular weight. Electrophoresis (SDS-PAGE) of seed storage protein can economically be used to assess, genetic variation and relation in genotypes. Thus, specific bands of seed storage protein profile may be used as markers for identification of the mutants/genotypes. The research results about the biochemical characteristics of desi chickpea varieties are expected to provide guidelines for the researchers confronted with the need to use such typical food seeds in India as well as in the rest of the world.
  Chaitali Sen and R.P. Singh
  The objective of the present study was to determine the effect of genotype and culture media in anther culture of boro rice hybrids. Anthers from three f1 hybrids of boroxhigh yielding indica rice varieties GautamxBPT 5204, Krishna HansaxNDR 359 and IR64xNDR 359 were cultured on modified SK media and modified N6 media. Modified SK media was found to be more suitable for callus formation in all the f1 hybrids. The cross GautamxBPT 5204 showed maximum callus induction frequency followed by Krishna HansaxNDR 359. Only the cross of Krishna HansaxNDR 359 was responsive to green plant regeneration while IR64xNDR359 and GautamxBPT 5204 resulted in only albino plants. Anther culture response is influenced by the genotype and media composition in indica rice hybrids.
  N. Shit , R.P. Singh , K.V.H. Sastry , J. Mohan , N. Pandey and R.P. Moudgal
  In sexually active male Japanese quail cloacal gland hypertrophy and foam production is androgen dependent and highly positively correlated with testis size and sexual activity. Nevertheless, the physiological functions of this gland in male reproduction are still a controversial issue. Therefore, the aim of this investigation was made to rule out the effect of cloacal gland size on semen production, sperm activities and fertility in both Heavy Body Weight (HBW) and White Feathered (WF) lines of Japanese quail. Sixty matured males and thirty females (8 weeks) from each lines of Japanese quail were randomly selected. Males were categorized into three groups (20/group) based on the increasing order of cloacal gland area. They were maintained in uniform husbandry condition with ad libitum feed and water at 14 h photo-schedule. Highest (11.04±0.20 and 9.6±0.41) semen production was recorded in birds having largest cloacal gland area and was positively correlated to the cloacal gland size in both the experimental quail lines. Metabolic activity by adopting Methylene Blue Reduction Time (MBRT) test and proteolytic activity by Acrosine Proteolytic Activity (APA) test of quail spermatozoa was found significantly different (p≤0.05) among groups and also revealed a positive correlation to the increasing area of the cloacal gland. In both the experimental lines, fertility was noted maximum (p≤0.05) in group III categorized by largest cloacal gland area. From this current study, it may be concluded that cloacal gland can be considered as external indicator or selection marker of testicular functions in male Japanese quail.
  K.P. Singh , J. Kumar , A. Singh , R.K. Prasad , R.P. Singh and D. Prasad
  Experiments were conducted to forecast the Potential Ascospore Dose (PAD) of the apple scab, caused by Venturia inaequalis (Cks.) Wint. (anamorph: Spilocaea pomi Fr.), was evaluated in Gangotri valley over 15 years (1994-2012) at four different place. In India, the disease causes significant economic losses in the states of Jammu and Kashmir and Himachal Pradesh each season and has been prevalent in Uttarakhand hills, the 3rd largest apple growing state in the country. Yield losses during epidemic years in 1996 and 2008 in the region went up to 70%. The PAD has great impact on disease development in the following season. It varied between 645-373421 in various orchards of Gangotri valley. The PAD value was low during 1999-2001 (645-1894 ascospore/m2) and medium during 2002-2006 (2108-29685 ascospore/m2) due to the fact that the springs were early, dry and unfavorable for ascospore maturation. In 1996 and 2008, the PAD values were high because of the favourable weather conditions and increase of inoculum accumulation. The scabbed lesion and leaf litter density were approximately more than twice in Gangotri fruit belt in 1996 and 2008. For this reason, reduction of primary inoculum sources played very important role in the improvement of scab management of apple in Uttarakhand Himalayas.
  K.P. Singh , J. Kumar , A. Singh , R.K. Prasad , R.P. Singh and D. Prasad
  Background: Apple scab, caused by Venturia inaequalis (Cke.) Wint. (anamorph Spilocaea pomi Fr.) is considered to be one of the most important fungal diseases of apple. In Uttarakhand Himalayas, the yield losses during scab epidemic years in 1996 and 2008 went up to 70%. Methodology: Random surveys were conducted during 1995-2014 from 3rd week of August until last week of September in apple orchards planted mostly to cv., Delicious. An apple scab forecasting and monitoring systems (μMETOS, Austria) installed at overwintering sites at Bhatwari fruit belt, Purola-Naugao, Tuni-Chakarata and Auli-Joshimath fruit belts recorded weather parameters including temperature, which were used to calculate degree-day accumulations. Results: The leaves falling early in the season appeared not to contribute much to the build-up of primary inoculum for the ensuing apple season. Leaves falling late had more scab lesions per leaf and posed a greater risk of increasing primary inoculums. The pseudothecia formation took 32-48 days more after sexual reproduction at temperature around 8-10̊C and this process was completed between February and March every year. The maximum ascospore productivity was recorded at fruit development stage of apple with ascospore productivity of 1,23,000 mL–1, which declined gradually. The ascospore emission period was 64-78 days and mean number of cumulative degree-days for 50 and 95% spore release were 456 and 960, respectively. Mills infection periods ranging from 19-47 were recorded from April-September every year that varied depending upon the weather conditions. The results revealed 2 day (light infection), 1 day (moderate infection) and 1 day (severe infection) delay in symptom expression under orchard conditions. Wetting requirement for infection to occur was also found to be more. More wetting of the leaves was required and symptoms were expressed within 7-9 days at higher temperature. Conclusion: The results of this study provide evidence that the use of average daily temperatures in spring to developed forecasting models based on degree-day accumulation could be used to predict the beginning and the end of the ascospore discharge during the apple growing season. The reduction of primary inoculum sources could have a decisive role in the management of apple scab.
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