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Articles by R.M. Widharna
Total Records ( 2 ) for R.M. Widharna
  K. Anam , R.M. Widharna and D. Kusrini
  Terminalia arjuna, Terminalia ballerica, Terminalia chebula, Terminalia catappa, Terminalia kaerbachii and Terminalia microcarpa leaves were tested for their α-glucosidase inhibitory activity in vitro. The α-glucosidase activity was determined by measuring the p-nitrophenol release from pNPG at 400 nm. Terminalia kaerbachii has the highest α-glucosidase inhibitor activity with IC50 value of 0.27±0.17 μg mL¯1 and is a promising antidiabetic herbal medicine candidate. However, most of the Terminalia species are also potential as antidiabetic medicine candidates as the IC50 values are approximately 5 μg mL-1, which is near the IC50 value of 1-deoxynojirimycin, the reference compound, except for Terminalia microcarpa, which has IC50 value of 25.15±0.04 μg mL-1 (above 21 μg mL-1). From the phytochemical screening, Terminalia kaerbacchi contains alkaloids, flavonoids and cathechic tannins, but does not contain saponin, quinon, steroid/terpenoids and gallic tannins. It is estimated that there is a correlation between α-glucosidase inhibitory activity and its phytochemical content.
  R.M. Widharna , A.A. Soemardji , K.R. Wirasutisna and L.B.S. Kardono
  Euphorbia hirta L. is a plant used in traditional medicine and proven pre-clinically for antiinflammation, asthma, wound healing and diarrhea. It also has antioxidant activity. However, the anti-diabetes mechanism of this plant has not been elucidated. A reliable technique to elucidate the anti-diabetes mechanism in the traditional medicine is required. Therefore, a new technique which was based on the glucose tolerance test was explored. Through various oral glucose tolerance test loading, the anti-diabetes properties of Euphorbia hirta L. could be elucidated. From the in vitro experiment, it was known that the ethanol extract and ethyl acetate fractions are the fractions with α-glucosidase inhibition activity, while n-hexane, chloroform, butanol and water fractions does not have α-glucosidase inhibitory effect. Therefore, only ethanol extract and ethyl acetate fractions were used for the in vivo tests. Based on the in vitro and in vivo test, Euphorbia hirta L. ethanolic extract and ethyl acetate anti-diabetes mechanism is related to its antioxidant capacity and also its α-glucosidase inhibitory properties.
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