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Articles by R.C. Sihag
Total Records ( 2 ) for R.C. Sihag
  Tejpal Dahiya , R.C. Sihag and S.K. Gahlawat
  Haematological profile of an animal is the reflection of its immunological status. Fishes are prone to infections by several pathogens which influence the immunity of the affected individuals. The effect of three probiotics on four haematological parameters viz., level of haemoglobin, total erythrocyte count, total leucocytes count hematocrit/packed cell volume of Indian magur (Clarius batrachus L.) was studied over a period of eight weeks. The level counts of all these four parameters decreased significantly in the blood of fish administered with pathogenic organisms as compared to the control; the fish administered with pathogenic organisms died after three weeks. The level counts of hematological parameters showed significant increase in the blood of fish treated with probiotics alone versus control and also in fish administered with pathogenic organisms versus probiotics. A comparison among the three probiotics revealed that, probiotic 1 was more effective than probiotic 3 and probiotic 2; the effect of latter stood at the lowest level.
  Parvati Sharma and R.C. Sihag
  The study was conducted for 8 weeks to determine pathogenic effect of different fish pathogens in Cirrihinus mrigal (C. mrigala) which was infected with EUS disease. The pathogenic organisms (bacteria and fungi) collected from the infected part of the disease fish. Isolation and screening of microbes were carried out with the help of a number of biochemical tests. A total of eight bacterial isolates were obtained from the diseased fish. Out of eight, six bacteria viz., Streptococcus grp Q1, Aeromonas hydrophilla, Shigella spp., Streptococcus faecalis, Cellobiosococcus sciuri, Micrococcus luteus were found to be pathogenic. The fungus, Aphanomyces invadens was also isolated from the diseased fishes. The pathogenicity of disease causing organisms was tested through in vitro and in vivo experiments in different treatments. The result of experiment was found to be significant at level of p≤0.05.
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