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Articles by R. Wickneswari
Total Records ( 4 ) for R. Wickneswari
  K. Nadarajah , C.Y. Choong , S.J. Leong and R. Wickneswari
  Calamus manan floral cDNA libraries were constructed for four stages of flowering in male and female plants, respectively. The Calamus manan inflorescence ESTs were generated to provided a better understanding of the flowering process through the identification of genes that are expressed in the floral tissues of this plant. The BLASTX homology search showed that 119 ESTs that were generated from this study had significant matches to unknown proteins and an additional 127 ESTs did not match any protein sequences in the NCBI database. Therefore, a motif search was carried out for the unknown ESTs to predict their putative functions. A total of 136 EST clusters were used in the motif analysis and the InterProScan software was chosen as the motif search tool. There were 66 types of motifs detected from this search. Based on the motifs detected within the query sequences, putative function predictions were successfully performed on 49 EST clusters.
  M.S. Nur Fariza , S.L. Pang , C.Y. Choong and R. Wickneswari
  This study examined DNA sequence variations of coding regions of Cinnamate 4-hydroxylase (C4H) and Cinnamyl Alcohol Dehydrogenase (CAD) in Acacia mangium and A. auriculiformis. cDNA fragments of C4H and CAD with size 1.5 and 1.3 kb, respectively were cloned into pGEM-T Easy Vector and were sequenced. Twenty eight Single Nucleotide Polymorphisms (SNPs) were identified in the coding region of C4H of which 8 caused changes in the amino acids or nonsynonymous mutations and 20 were synonymous mutations. Thirty two SNPs were detected in coding region of CAD. Of these, 12 were nonsynonymous mutations and 20 were synonymous mutations. Two A. mangium individuals (M20 and M22) and two A. auriculiformis individuals (A6 and A3) were used as parents for generating F1 mapping populations. Nucleotide sequence alignment of coding region of CAD detected 28 and 22 SNPs from A3xM22 parental combination and A6xM20 parental combination respectively. Nucleotide sequence alignment of coding region of C4H identified 6 and 23 SNPs for A3xM22 and A6xM20 parental combinations, respectively. For parental combination A3xM22, 11 nonsynonymous mutations were detected while for A6xM20 parental combination 23 nonsynonymous mutations were detected from CAD gene. Amino acid sequence alignment of C4H detected 4 amino acid variations from each parental combination. The putative SNPs can be developed as SNPs markers for Quantitative Trait Loci (QTL) detection. Selecting favourable alleles from progenies which produce desirable lignin profiles would be advantageous in tree breeding programmes for plantation establishment.
  C.Y. Choong , R. Wickneswari and S. Fatimah
  Calamus palustris Griff. is a dioecious rattan species of commercial importance for its canes. The sex of C. palustris can only be identified from the morphology of the inflorescence. In this survey, the sex ratio of C. palustris planted in 2001 in an experimental plot at Universiti Kebangsaan Malaysia, was studied. The survey was conducted from September 2003 to July 2006. A total of 46 male and 43 female plants were identified from the 140 surviving plants in the plot. This gave a ratio of female to male as 1:1.1 with no significant difference compared to an even ratio 1:1. The survival percentage of the planted population was 93.3%. The female plants had 1-7 stems per clump, while the male had 1-10 stems per clump with an average of 4 stems per clump for both the sexes. The even sex ratio of the planted C. palustris population might indicate absence of post zygotic sex selection and unbiased sex selection during seed germination.
  J.L. Chong , R. Wickneswari , B.S. Ismail and S. Salmijah
 

This study reports the results of the partial DNA sequence analysis of the 5-enolpyruvyl-shikimate-3-phosphate synthase (EPSPS) gene in glyphosate-resistant (R) and glyphosate-susceptible (S) biotypes of Eleusine indica (L.) Gaertn from Peninsular Malaysia. Sequencing results revealed point mutation at nucleotide position 875 in the R biotypes of Bidor, Chaah and Temerloh. In the Chaah R population, substitution of cytosine (C) to adenine (A) resulted in the change of threonine (Thr106) to proline (Pro106) and from C to thymidine (T) in the Bidor R population, leading to serine (Ser106) from Pro106. As for the Temerloh R, C was substituted by T resulting in the change of Pro106 to Ser106. A new mutation previously undetected in the Temerloh R was revealed with C being substituted with A, resulting in the change of Pro106 to Thr106 indicating multiple founding events rather than to the spread of a single resistant allele. There was no point mutation recorded at nucleotide position 875 previously demonstrated to play a pivotal role in conferring glyphosate resistance to E. indica for the Lenggeng, Kuala Selangor, Melaka R populations. Thus, there may be another resistance mechanism yet undiscovered in the resistant Lenggeng, Kuala Selangor and Melaka populations.

 
 
 
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