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Articles by R. Samiyappan
Total Records ( 4 ) for R. Samiyappan
  L. Rajendran , G. Karthikeyan , T. Raguchander and R. Samiyappan
  In this study, for the management of Basal Stem Rot (BSR) disease sixty endophytic, rhizosphere strains were isolated from coconut, other crops and virgin soils. The strains showed high growth promotion were subjected to Ganoderma mycelium inhibition study in vitro. The strains EPC5 and EPC8 were showed high growth promotion and strong inhibition to Ganoderma pathogen compared to other strains. Both the strains were characterized by biochemical methods and confirmed as Bacillus. The Bacillus ITS region was amplified by specific primers and EPC5, EPC8 showed amplification of 546 bp products in size. Further, the strains were cloned and sequenced, deposited in NCBI, USA. The sequence showed similarity with Bacillus subtilis.
  L. Rajendran , G. Karthikeyan , T. Raguchander and R. Samiyappan
  .
  R. Nandakumar , S. Babu , G. Amutha , T. Raguchander and R. Samiyappan
  Phytotoxic metabolite produced by the five different isolates of Sarocladium oryzae, the rice sheath rot pathogen, was isolated from its in vitro culture filtrate to know the variability among isolates in terms of toxin production. The toxin was purified through solvent extraction and then by gel filtration on sephadex column. Carbohydrate and protein content of the toxin and the quantity of phytotoxin produced in planta were varied among isolates. Toxin from the five isolates also showed variation in its biological activity as detected in its ability to induce sheath rot symptoms on detached leaf sheath, greenhouse grown plants and to cause leakage of electrolytes from rice leaf sheath. The symptoms produced by the purified toxin were, symptoms similar to those of natural infection by the pathogen. The virulent isolates SO1 and SO2 produced more amount of toxin, more leakage of electrolytes and severe sheath rot symptoms. The in planta toxin production by different isolates was detected serologically, using the polyclonal antibody produced against SO1 toxin. Correlation between the variation in toxin production and the virulence of the pathogen was discussed.
  L. Rajendran and R. Samiyappan
  Endophytic bacterial strains were evaluated for their efficacy against the damping off disease caused by Rhizoctonia solani in cotton. Among hundred and three endophytic bacterial strains isolated, two strains (Bacillus sp. strains EPCO102 and EPCO16) significantly increased plant growth and inhibited the mycelial growth of Rhizoctonia solani in vitro conditions. The efficacy of talc-based bioformulation of endophytic bacterial strains, EPCO102, EPCO16 and Pseudomonas fluorescens strain Pf1 amended with and without chitin in inducing systemic resistance was tested against damping off disease under greenhouse conditions. The application of the bioformulation through seed, soil and foliar spray significantly reduced disease incidence under greenhouse conditions. The amendment of chitin in the formulation further reduced the disease incidence. EPCO102, EPCO16 and Pf1 strain along with chitin treatment was recorded 46.7, 53.3 and 40.0% damping-off incidence compared to control 83.3%. Treatment with the endophytic bacterial bioformulation increased the levels of the defence-related enzymes chitinase, β-1,3-glucanase, peroxidase, polyphenol oxidase, phenylalanine ammonia-lyase and phenol in cotton plants which had been challenged with R. solani. In addition to plant growth and antibiosis, endophytic bacterial strains enhanced the resistance in plants through the induction of defense enzymes in cotton plants.
 
 
 
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