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Articles by R. Roy
Total Records ( 3 ) for R. Roy
  P. K. Rout , A. Kumar , A. Mandal , D. Laloe , S. K. Singh and R. Roy
  Milk protein polymorphism plays an important role in genetic diversity analysis, phylogenetic studies, establishing geographical diversity, conservation decision, and improving breeding goals. Milk protein polymorphism in Indian goat breeds has not been well studied; therefore, an investigation was carried out to analyze the genetic structure of the casein gene and milk protein diversity at six milk protein loci in nine Indian goat breeds/genetic groups from varied agro-climatic zones. Milk protein genotyping was carried out in 1098 individual milk samples by SDS-PAGE at αS1-CN (CSN1S1), β-CN (CSN2), αS2-CN (CSN1S2), κ-CN (CSN3), β-LG, and α-LA loci. Indian goats exhibited αS1-casein A allele in higher frequency in the majority of breeds except Ganjam and local goats. The αS1-casein A allele frequencies varied from 0.45 to 0.77. A total of 16 casein haplotypes were observed in seven breeds and breed specific haplotypes were observed with respect to geographic region. The average number of alleles was lowest in Ganjam (1.66 ± 0.81) and highest in Sirohi goats (2.50 ± 1.05). Expected heterozygosity at six different loci demonstrated genetic diversity and breed fragmentation. Neighbor-Joining tree was built basing on Nei's distance. There was about 16.95% variability due to differences between breeds, indicating a strong subdivision. Principal component analysis was carried out to highlight the relationship among breeds. The variability among goat breeds was contributed by αS2-CN, β-LG and αS1-CN. The Indian goats exhibited αS1-CN (CSN1S1) A allele in higher frequency in all the breeds indicating the higher casein yield in their milk.
  S. Pujari , S. Bhosle and R. Roy
  The natural distribution of two essential fatty acids for animal, namely linoleic and alpha linolenic acid, is not in same order. While the distribution of linoleic acid is cosmopolitan, alpha linolenic acid distribution is very much restricted more confined to marine environment. The seven days old chicks of Gallus domesticus, broiler strain, were fed a diet supplemented with Staphylococcus, Streptococcus and Pseudomonas bacterial strains, collected from the west coast of India, for a period of 30 days. These bacterial strains contained about 15-20% of alpha linolenic acid (as essential fatty acid) when grown on sodium acetate medium. Dietary supplementation of these bacteria increases the net weight of the birds in comparison to the control birds. This net weight gain of the bird was also reflected in total protein, triglycerides, cholesterol and phospholipid concentrations of various tissues. Supplementation of these bacteria as a source of alpha linolenic acid in birds enhanced the tissue level eciosapentaenoic and docosahexaenoic fatty acids at the cost of palmitic, oleic, linoleic and arachidonic acid. A reduction in the serum total cholesterol, total triglycerides along with an increase of HDL cholesterol was noticed in the birds whose diet was supplemented with Streptococcus strain of bacteria only. The cardiac and liver function tests confirmed the well being status of the birds supplemented with Streptococcus strain. The involvement of dietary alpha linolenic acid towards the metabolism of cholesterol in birds was noticed in the Gallus.
  R. Roy , S. Singh and S. Pujari
  The 7 days old chicks, Gallus domesticus were fed with a diet supplemented with 2.5%, 5% and 10% of ω-3 enriched PUFA (containing 180mg of eciosapentaenoic acid and 120mg docosahexaenoic acid per gram oil) for a period of 30 days. Dietary supplementation of PUFA promotes the growth of the birds that was reflected in the elevation of tissue protein, cholesterol and phospholipid along with a reduction in tissue triglycerides concentrations. Accumulation of ω-3 PUFA along with the depletion of ω-6 PUFA, oleic acid, myristic acid and stearic acid in the tissues was detected. Supplementations of 10% ω-3 enriched PUFA promote the health status of the bird as evident from 20% increase in the haemoglobin concentration of blood, 60% decrease in the serum LDH activity and with no change in the serum cholesterol profiles. 75% reduction in HMG CoA reductase activity along with 62% augmentation of the HMG CoA synthase activity in the liver was recorded which suggest the alteration of cholesterol metabolism in the bird.
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