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Articles by R. Ranjbar
Total Records ( 9 ) for R. Ranjbar
  R. Ranjbar , S.H. Rashidi , N. Alboghobeish , R.A. Sadrkhanloo and Y. Mazaheri
  This study was Performed on 102 embryos and fetuses that collected from Ahvaz slaughter house. The specimens was divided in three group, on the basis of external genitalia differentiation and Crown Rump Length (CRL), as groups 1, 2 and 3. The embryos of group 1 (No. 63, CRL-10-83 cm) showed completely developed external gentalia of male and female, so there was no doubts in the recognition of their sexes. The embryos of group 2 (No. 19, CRL-4-10 cm) also showed clear sex on the basis of genital tubercle (clitoris in the female and penis in the male embryos) and genital swellings (lips of vulva in the females and scrotom in the males). The tip of genial tubercle of females embryos have showed dorso-caudally and of males ventro-cranially directions, respectively. In the embryos of group 3 (No. 20, CRL = 0.38-4 cm). The determination of sex was not possible by help of external genitalia. So, the gonads of these embryos, were removed from abdominal cavity and after histological preparations, stained by H and E and PAS methods. On the basis of histological studies, sex differentiation of gonads to testis and ovary was recognized in embryos with CRL = 2.1 cm and CRL = 2.3 cm, respectively. Also the gonads of embryos lesser than CRL = 2.1 cm, recognized as undifferentiated gonads. These findings indicated that, sex differentiation in the level of gonads start sooner than external genitalia and also the differentiation of sex in male embryos has priority to female embryos. In addition, the embryos show an undifferentiated stage, that is impossible to recognize the sex of embryos neither on the basis of external genital nor gonadal histological structure.
  R. Ranjbar and A.L. Ghadiri
  The aim of study was to evaluate of intrahepatic ramifications of portal vein in water buffalo of Iran. Apparently 12 healthy and fresh livers of were selected from slaughterhouse and transferred to anatomy and radiology labs. To remove and discharge of blood clots, the portal vein of livers was perfused with normal saline. Then the livers divided in 3 groups and portal veins were studied by using corrosion cast, gross dissection and radiological examination. The results revealed that the portal vein divided at porta in to right and left interlobar branches. The right interlobar branch was short and large and the left branch was long and narrow. Right and left interlobar branch gave dorsal, intermediate and ventral interlobular veins and transverse and umbilical parts, respectively. Dorsal interlobular vein supplied dorsal part of right lobe, papillary and caudate processes of caudate lobe. Ventral interlobular vein divided in 3 branches that distributed in ventral parts of right and quadrate lobes. The intermediate interlobubar vein distributed in the diaphragmatic parts of right lobe and papillary process. The transverse part of left branch gave off several branches to papillary process of caudate lobe and few branches to quadrate lobe. The umbilical part of left gave off 3 branches, the dorsal, intermediate and ventral interlobular branches that radiated in to the left and quadrate lobes. Therefore, the pattern of intrahepatic branches of portal vein in water buffaloes of Iran is similar to other reports about water buffaloes, cattle and dog, less or more.
  M.H. Shirazi , R. Ranjbar , S. Eshraghi , G. Amin , M. Seyed Nouri and N. Bazzaz

Antibacterial activity of Sage extract at concentrations of 0.1, 0.05, 0.025, 0.0125, 0.00625, 0.003125, 0.00156, 0.0005 and 0.00025 g dL-1 against Salmonella typhi, Shigella sonnei, S. flexneri, Proteus vulgaris, Staphylococcus aureus, ETEC Escherichia coli and Pseudomonas aeruginosa was evaluated. Susceptibility testing of bacterial strains against 18 antibiotics was also performed for comparison. The results showed that P. aeruginosa and ETEC E. coli were completely resistant to Sage extract even at concentration of 0.1 g dL-1. Its antibacterial activity (0.1 g dL-1) against P. vulgaris, S. flexneri and S. sonnei was the same as nitrofurantoin and ampicilline respectively. Sage extract (0.1 and 0.05 g dL-1) exhibited the same effects as ampicilline and streptomycin against S. typhi. Its antibacterial activity (0.1, 0.05 and 0.25 g dL-1) against S. aureus was the same as ceftazidim, chloramphenicol, gentamycin, neomycin and nitrofurantoin and was more significant compared to streptomycin and vancomycin. The results suggest Sage can be considered as an alternative herbal in the treatment of infections caused by the above-mentioned bacteria.

  E. Salimkhani , R. Ranjbar , N. Sadeghifard , S. Morovvati , N. Jonaidi and M. Izadi
  Abstract: Aim of this study was to determine the causative agent and source of a large gastroenteritis outbreak occurred in a national financial center (CBIRI) in July 2003. A patient definition was defined staff were interviewed in the clinic of the Bank and their information were collected by means of a standardized questionnaire. A total of 110 fecal specimens were collected within 48 h of symptom onset from 100 patients with symptoms of gastroenteritis and 10 restaurant staff. The specimens were processed within 12 h to detect ova and parasites by direct microscopy and common bacteria by standard methods. The outbreak started on 22 July 2003 lasted 4 days. From a total of 1300 staff. 535 persons experienced a severe gastrointestinal illness. None but one of tested fecal samples were positive for bacterial enteric pathogens. S. paratyphi B was isolated from the positive case. Definitive association between illness and isolated S. paratyphi B remained to be determined since it was isolated only from one case. There is a need, however, for increased awareness among both professionals and the public to implement appropriate prevention measures and monitoring of food and water.
  R. Ranjbar , P. Owlia , H. Saderi , Z. Bameri , M. Izadi , N. Jonaidi and S. Morovvti
  Aim of this study was to investigate the presence of plasmids among the strains of P. aeruginosa isolated from clinically diagnosed cases in Tehran in 2006. A total of 38 strains of P. aeruginosa were isolated. With the exception of one isolate, all P. aeruginosa strains harbored at least one plasmid band. The electrophoretic analysis of plasmid DNAs showed different number of plasmid bands among the strains tested. The DNA band of 1.4 kbp was evident in 84.2% of the strains. Approximately 71 and 21% of the isolates harbored concomitantly two and three plasmids, respectively. Isolation of strains with diverse types of plasmids suggests the different cluster of P. aeruginosa might be disseminated during the current study period.
  A. Ghasemi , M.H. Shirazi , R. Ranjbar , M.R. Khorramizadeh , N.E. Daryani and M. Hosseini
  The aim of this study was to investigate the prevalence of cagA and cagE genes in H. pylori strains isolated from different patient groups with Non-Ulcer Dyspepsia (NUD), Duodenal Ulcer (DU), Gastric Ulcer (GU) and Gastric Cancer (GC). The patients admitted to the gastroenterology unit at Sharyati hospital in Tehran in 2006 were included in this study. Gastric biopsy specimens were obtained from the antrum of the stomach from each patient then cultured for detection of H. pylori. Identification of H. pylori was performed according to the standard bacteriological methods. Genomic DNA was extracted using a commercially available Qia gene kit. PCR was done using primers cagA-F, cagA-R and cagE-F, cagE-R to detect the target genes cagA and cagE, respectively. Amplified products of target genes were confirmed by sequencing. The cagA and cagE were detected among 85 and 86% of H. pylori isolates, respectively. Prevalence of cagA and cagE genes in the patients with NUD, DU, GU and GC were 22 (64.7%), 28 (100%), 18 (90%), 10 (100%) and 25 (73.5%), 27 (96.4%), 19 (95%), 7 (70%), respectively. The current study demonstrated a significant correlation between peptic ulceration and the presence of H. pylori isolates carrying cagE and cagA genes in Iranian patients.
  R. Sorouri , R. Ranjbar , N. Jonaidi Jafari and A. Karami
  This study was carried out to evaluate the nested PCR for specific detection of different strains of B. burgdorferi. Five strains of B. burgdorferi including ACA-1, B-31, 2B45, 3B45, 7B49 obtained from different countries were used in this study. The strains of B. hermsii, Escherichia. coli and T. pallidum were also included as control strains. Two pairs of nested PCR primers were used to amplify the gene encoding the Osp-A protein of B. burgdorfer under standard PCR condition. In a two stage procedure, nested PCR yielded a positive reaction for five tested strains of B. burgdorferi. None the strains including B. hermsii, E. coli and T. pallidum showed positive reaction when used as control strains in PCR. In conclusion, nested PCR showed acceptable specificity for rapid detection of B. burgdorferi.
  N. Jonaidi Jafari , R. Ranjbar , M.T. Haghi-Ashtiani , M. Abedini and M. Izadi
  The aim of this study was to investigate the prevalence and antimicrobial susceptibility of bacterial strains isolated from tracheal specimens obtained from pediatric patients admitted to a major children hospital in Tehran, in 2007. Tracheal specimens were cultured on the appropriate bacteriological media. Bacterial isolates were identified by standard biochemical and serological tests. Antimicrobial susceptibility testing was performed according to Clinical and Laboratory Standards Institute (CLSI) guidelines. Pseudomonas spp. was identified as the most prevalent bacterial isolate (32%) followed by Staphylococcus aureus (27.6%). Thirty strains (16%) were identified as Klebsiella spp., 18 (9.6%) as Enterobacter spp. and the rest belonged to coagolase negative Staphylococci, Streptococcus viridans, Acinetobacter spp., Escherichia coli and Neisseria spp. All Pseudomonas spp. were resistant to ampicllin, kanamycin and ceftizoxime. Staphylococcus and Klebsiella spp. showed high degree of resistance to 40% of examined antibiotics.
  F. Khorvash , A.A. Javadi , M. Izadi , N. Jonaidi Jafari and R. Ranjbar
  The purpose of this study is to review of spinal tuberculosis in three hospitals in Isfahan of Iran. We carried out a cross sectional study of 630 patients with tuberculosis and identified 100 patients with spinal involvement in the three hospitals. Tuberculosis was diagnosed based on one of a compatible clinical picture. A radiographic study of the spine with suspicious signs and skin tested were performed for each patient. Demographic data, sign, symptoms and site of spinal involvement were recorded. In all patients, a chest X ray and sputum smear and culture was performed for rouling out of pulmonary tuberculosis. Out of the 100 patients with spinal tuberculosis, 58% were male and 42% were female. Main symptoms were spinal deformity, local tenderness and neurologic deficits. Fever and constitutional symptoms were in 80% of cases. Only 68% had a positive tuberculin skin test. Three percent involvement were the upper thoracic spine, 23% the lower thoracic spine, 69% also the lower thoracic, T12 and upper lumbar spines, (thoracolumbar) and 5% the cervical spine. 40 cases underwent bone biopsy that 25% had a positive smear, whereas 62.5% had a positive culture. Histologic findings suggestive of tuberculosis involvement of the bone were found in 37 of the 40 biopsies. The most common age for spinal involvement were 20-40 years (p< 0.05). Spinal tuberculosis may be missed in patients with no evidence of pulmonary. No pathognomonic imaging signs allow tuberculosis to be readily distinguished from other conditions. In this here, we discuss about clinical and histopathological findings in patients with spinal tuberculosis.
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