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Articles by R. Muthezhilan
Total Records ( 4 ) for R. Muthezhilan
  N. Yogananth , R. Bhakyaraj , M. Syed Ali and R. Muthezhilan
  Background and Objective: Kaempferol is the important polyphenolic flavonoids that is used to the remedy for cancers, cardiovascular disease, neuron disorder, cholesterol and serve as antioxidant and anti-inflammatory. Materials and Methods: The present investigation was the comparative analysis of kaempferol from field grown plant parts and in vitro callus and also the enhancement of kaempferol by the influence of yeast elicitor were determined. In the present study, at the concentration of 2.0 mg L1 2,4–D+0.5 mg L1 KIN gave the maximum callus growth for both stem and leaf explants (3.47±0.50e and 3.56±0.44e, respectively). Results: The maximum content of kaempferol in three type of samples was observed in this order; yeast elicitor treated samples > in vitro raised callus samples > field grown samples. The highest amount of kaempferol was fount in 1 mL yeast elicitor treated with stem callus sample (0.986 mg g1 dw). Conclusion: Therefore, the present finding it was proved that the highest accumulation of kaempferol in elicitor treated in vitro callus is provides resource for preparation of drugs against various diseases by the pharmaceutical industries.
  R. Muthezhilan , B.S. Sindhuja , A. Jaffar Hussain and M. Jayaprakashvel
  Plant Growth Promoting Rhizobacteria (PGPR) are beneficial bacteria that colonize the plant root and enhance the plant growth. The use of PGPR is steadily increasing in agriculture and offers an attractive way to replace chemical fertilizers, pesticides and supplements. In the present study, PGPR were isolated from 18 different rhizosphere soil samples of coastal sand dune plants, belonging to the genus Ipomoea sp. collected from the Chennai coastal area. For isolation of bacteria from soil samples, pour plate technique was followed. The rhizobacterial population was ranged from 4.4x106-7.5x107 CFU g-1. From that, 46 morphologically different bacterial strains were isolated. Among 46, 18 strains exhibited the production of Indole Acetic Acid. (IAA). When screened for phosphate solubilzing activity, six strains showed maximum activity. All these selected six strains were screened for seed germination among which these two strains (AMET1136 and AMET 1148) showed remarkable increase in the seed germination of black gram and green gram. For plant growth promotion, three types of treatments namely, seed bacterization, soil drenching and mixed (seed+soil) were carried out to check the potential of these two strains. Among that one strain which was identified as Pseudomonas sp. AMET1148 showed remarkable and significant increase in shoot length and root length of the tested plants. The study concluded that PGPR from coastal sand dund plants can be developed as plant growth promoters in agricultural crops.
  R. Muthezhilan , N. Yogananth , S. Vidhya and S. Jayalakshmi
  The present study deals with the distribution of fungal species in Cuddalore dye industrial waste (Lat 11°42`N; Long 79°46`E) and their dye degrading activity. Totally 13 species under 17 genera were isolated and screened for their decolorization activity against methylene blue, gentian violet, crystal violet, cotton blue, Sudan black, malachite green, methyl red and corbol fushion in mineral salt medium and Czepex-Dox broth. In agar medium, decolorization began with the formation of zone of clearance around the colonies. Aspergillus ochraceus, A. terreus, A. niger, Penicillium citrinum and Fusarium moniliforme decolorized maximum number of dyes to a great extent. Mucor racemosus, Cladosporium cladosporioides, Penicillium oxalicum and Trichoderma viride did not decolorize any of the dyes tested. In liquid medium, decolorizing activity was measured spectrophotometrically. Aspergillus ochraceus, A. terreus, A. niger, Penicillium citrinum and Fusarium moniliforme registered maximum color reduction, where as Mucor racemosus, Cladosporium cladosporioides, Penicillium oxalicum and Trichoderma viride expressed very low amount of color reduction. Biomass and the extent of dye removal are directly propositional. Among the 13 species of fungal isolates, Aspergillus ochraceus, A. terreus, A. niger, Fusarium moniliforme and Penicillium citrinum seems to be potential candidates for dye degradation. These strains can be used for the bioremediation of environs polluted with dye effluents.
  N. Yogananth , M. Akila , M. Syed Ali , M. Jayaprakashvel and R. Muthezhilan
  Background and Objective: Now a days, the need for remarkable disease resistance, growth of aquatic organisms and feed competence has brought about the employ of probiotics in aquaculture field. The objective of the present study was to isolate probiotic bacterial strains from fish intestines and screen them by in vitro testing of their antagonism to pathogens and bioencapsulation efficacy. Materials and Methods: Six bacteria were isolated from marine fish gut and identified by biochemical test. All six isolates were assessed in vitro for its inhibitory activity against common fish pathogens of Aeromonas hydrophila and Vibrio harvey by well diffusion assay. Based on the antimicrobial activity, the Enterococcus, Streptococcus and Bacillus were selected for assaying probiotic properties of acid tolerance, bile salt concentration and auto aggregation assay. The 24 h old Artemia salina nauplii were selected for probiont encapsulation study. Results: The isolates Enterococcus, Streptococcus and Bacillus were confirmed as probionts by above mentioned three methods. The research of encapsulation studies exposed that the gut loading and evacuation time of Artemia nauplii differed according to the oils and probiotics used for the trials. Probiotics take less time for gut loading and retention when compared to oils. Conclusion: The findings of this study indicated that the indigenous fish gut bacteria provided benefit to the culture fishes in terms of pathogen protection and increasing growth potential and thus fulfilled the major requirements of being effective probiotics
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