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Articles by R. Kumar
Total Records ( 10 ) for R. Kumar
  R. Kumar , A. Sinha , S. Srivastava and M. Srivastava
  The aim of the present study was to investigate the variation in soil mycobiota involved in the decomposition of Sesbania aculeata L. in soil. Decomposition of Sesbania aculeata (Dhaincha) was studied by nylon net bag technique under experimental conditions. The colonization pattern by soil inhabiting mycobiota was studied by standard methods. Among the three methods used for isolation and enumeration of fungi, dilution plate technique recorded the highest number of fungi followed by damp chamber and direct observation method. Nutrient availability and climatic conditions influenced occurrence and colonization pattern of mycobiota. Maximum fungal population was recorded in July (48.95±0.20x104 of fungi/g oven dry litter) and minimum in June (19.78±0.20x104 of fungi/g oven dry litter). The distribution of Deuteromycetous fungi was much more (74.47%) than Zygomycetes, Oomycetes and Ascomycetes. In the early stage of decomposition Mucor racemosus, Rhizopus stolonifer, Chaetomium globosum and Gliocladium roseum were found where as at the later stages of decomposition preponderance of Aspergillus candidus, Cladosporium cladosporioides, Curvularia lunata and Aspergillus luchuensis was recorded.
  S. Srivastava , V.P. Singh , R. Kumar , M. Srivastava , A. Sinha and S. Simon
  The aim of the present study was to check the efficacy of carbendazim 50% WP, antagonists and botanicals against Fusarium oxysporum f. sp. psidii associated with rhizosphere soil of guava. Guava (Psidium guajava L.) the apple of tropics is one of the most important fruit in India. The fruit of guava is a good source of vitamin C and pectin. Guava wilt is a serious disease and it recognized as a main causal organism. Isolation and identification of pathogen (Fusarium oxysporum f. sp. psidii) was carried out in the Department of Plant Protection, Allahabad Agricultural institute Deemed University, Allahabad. The radial growth of Fusarium cxysporum f. sp. psidii was fully inhibited at high concentrations like 100, 1000 and 10,000 ppm of cardendazim 50% WP whereas antagonists like Trichoderma spp. Produced maximum inhibition zone (61.91%) followed by Aspergillus niger (61.12%). The radial growth of Fusarium cxysporum f. sp. psidii was also significantly less in neem leaf extract treatment followed by Lantana leaf extract. Management of guava wilt by chemical (systemic fungicide) can be spectacular but this is relatively short-term measure. Eco-friendly management practices, i.e., use of bio-control agents and botanicals was studied in vitro which gave better results and these practices can be economical, long lasting and free from residual side effects.
  V.K. Gupta , J. Singh , R. Kumar and A. Bhanot
  The aim of this research was to study the pharmacognostic parameters of leaves of Ocimum gratissimum L. (family: Lamiaceae). Macroscopic study showed that the leaves of this plant possess dark green color with characteristic aromatic odour and bitter taste. Powder microscopy showed the presence of numerous glandular simple trichomes of average length 101 μm and anomocytic stomata were also present, whereas, T.S of leaf showed that the leaf has a pot shape midrib and a thin lamina with uneven lower epidermis attached at the lateral sides of its upper side leaving a concave central dorsal depression. Both upper and lower epidermis showed simple, covering, uniseriate trichomes as well as sessile short stalked glandular trichomes. Quantitative microscopy viz., veinlet termination number, vein islet number, stomatal number, stomatal index; physico-chemical parameters viz., ash values, extractive values, Thin layer chromatography (TLC); florescence analysis of Ocimum gratissimum L. were also been determined. TLC studies showed the presence of various phytochemicals in the extracts. Qualitative phytochemical analysis revealed the presence of alkaloids, tannins, flavonoids, terpenoids (methanolic, ethanolic extracts); alkaloids, flavonoids and terpenoids (petroleum ether, chloroform extracts) and carbohydrates in alcoholic extract. This is the first such study on standardization of Occimum gratissimum L. leaf which would serve as a standard reference for identification and distinguishing the plant from its adulterants.
  J. C Seegmiller , D. R Barnidge , B. E Burns , T. S Larson , J. C Lieske and R. Kumar

Background: Urinary albumin excretion is a sensitive diagnostic and prognostic marker for renal disease. Therefore, measurement of urinary albumin must be accurate and precise. We have developed a method to quantify intact urinary albumin with a low limit of quantification (LOQ).

Methods: We constructed an external calibration curve using purified human serum albumin (HSA) added to a charcoal-stripped urine matrix. We then added an internal standard, 15N-labeled recombinant HSA (15NrHSA), to the calibrators, controls, and patient urine samples. The samples were reduced, alkylated, and digested with trypsin. The concentration of albumin in each sample was determined by liquid chromatography–tandem mass spectrometry (LC-MS/MS) and linear regression analysis, in which the relative abundance area ratio of the tryptic peptides 42LVNEVTEFAK51 and 526QTALVELVK534 from albumin and 15NrHSA were referenced to the calibration curve.

Results: The lower limit of quantification was 3.13 mg/L, and the linear dynamic range was 3.13–200 mg/L. Replicate digests from low, medium, and high controls (n = 5) gave intraassay imprecision CVs of 2.8%–11.0% for the peptide 42LVNEVTEFAK51, and 1.9%–12.3% for the 526QTALVELVK534 peptide. Interassay imprecision of the controls for a period of 10 consecutive days (n = 10) yielded CVs of 1.5%–14.8% for the 42LVNEVTEFAK51 peptide, and 6.4%–14.1% for the 526QTALVELVK534 peptide. For the 42LVNEVTEFAK51 peptide, a method comparison between LC-MS/MS and an immunoturbidometric method for 138 patient samples gave an R2 value of 0.97 and a regression line of y = 0.99x + 23.16.

Conclusions: Urinary albumin can be quantified by a protein cleavage LC-MS/MS method using a 15NrHSA internal standard. This method provides improved analytical performance in the clinically relevant range compared to a commercially available immunoturbidometric assay.

  R. Kumar , S. Malarvizhi and S. Jayashri
  Orthogonal Frequency Division Multiplexing (OFDM) is a modern high data rate modulation technique for wireless communication systems. Due to loss of orthogonality of subcarriers intercarrier interference occurs. In this study the performance of the proposed time-domain equalization technique is compared with the existing frequency domain equalization technique. In frequency domain equalization a correlative polynomial is used in the frequency domain to suppress the ICI. In Time domain equalization technique, a window function is proposed in equivalent to the correlative polynomial used in the frequency domain. MATLAB simulation of the proposed technique shows that the time domain windowing scheme achieves better performance in ICI suppression compared to the correlative coding technique. Time domain windowing technique proposed in this study, offers better Carrier to Interference Ratio (CIR) and BER is reduced compared to the correlative coding method.
  A.B. Sharangi and R. Kumar
  Black pepper (Piper nigrum), known as “King of spices”, is a perennial export oriented cash crop in India. With organic spice being the latest demand by the health conscious people throughout the globe, organic substitution of nitrogenous fertilizers in black pepper is becoming relevant day by day. An investigation was carried out to study the influence of different organic substitution of nitrogenous fertilizers in nutrient schedule on different growth parameters of the black pepper cultivar Panniyur-1. Recommended dose of P and K in the form of SSP and MoP was given to the vines. The plants were allowed to grow for upto 36 months of age for taking observations on different growth parameters. Considering the realization of highest response for four important growth parameters viz., plant height (269.37 cm), plant fresh weight (533.80 g), plant dry weight (178.01 g) and relative growth rate, RGR (5.10 g g-1 day-1) after 36 months of planting, it may be concluded that the organic matter supplementation by 25% farm yard manure along with 75% urea) may be the best nutrient schedule under this agro-climatic condition.
  R. Kumar , R.M. Reddy , P.S. Sinha , J. Tirkey , M.K. Singh and B.C. Prasad
  The continuous exploitation of tropical tasar food-plant, Terminalia tomentosa (W and A) rose on wastelands under the rain-fed conditions by rearing its economic insect pest; Antheraea mylitta (D) needs soil management for sustainable yields. To asses the feasibility, legumes like Sun hemp (Crotalaria juncea), Daincha (Sesbania aculeata), Green gram (Vigna radiatus) and Black gram (Vigna mungo) were raised during monsoon (June) season in the inter space of T. tomentosa economic plantation for biomulching. The Sun hemp has yielded highest biomass of 39.0 q ha -1, followed by Daincha, Green gram and Black gram with 26.6, 25.3 and 17.7 q ha -1, respectively after 45 to 50 days of sowing, has been mulched for soil fortification. The water holding capacity, electrical conductivity and organic carbon have been improved with reduced pore space and bulk density of the soil and enhanced leaf yield of tasar food-plant after legume biomulching. The superior Effective Rate of Rearing (ERR), higher cocoon and shell weights, better silk ratio and longer silk filament length of tasar silkworm in biomulch treatments have contributed to increase silk yield. Among the biomulches, Sun hemp has shown improved water holding capacity (56.9%) and moisture (68.5 and 37.9%) at 30 and 45 cm depth with electrical conductivity (0.30 mhos cm -1), bulk density (1.12 g cm -3), pore space (50.4%), organic carbon (0.61%) of soil, leaf yield (24.8 MT ha -1) of food plant and ERR (80.1%), cocoon weight (12.25 g), shell weight (1.90 g), silk ratio (14.03%) and silk filament length (724 m) specify its suitability in making tropical tasarculture sustainable under rain-fed conditions.
  K Ohshiro , P Mudvari , Q. c Meng , S. K Rayala , A. A Sahin , S. A. W Fuqua and R. Kumar

Alternative splicing of precursor mRNA is a fundamental mechanism to generate multiple proteins from a single gene. Although constitutive and alternative mRNA splicing is temporally and spatially regulated, deregulation of mRNA splicing could cause development, progression, and metastasis of tumors. Through yeast two-hybrid screening of a human breast cDNA library using estrogen receptor- (ER) as bait, we identified a novel nuclear receptor box containing full-length protein, nuclear protein E3-3 (NPE3-3). Our results revealed that NPE3-3 associates with not only ER but also with splicing factors, serine/arginine-rich protein (SRp)-30c, SRp40, and splicing factor SC-35, suggesting that NPE3-3 is likely to be involved in regulation of mRNA splicing. Accordingly, transient expression of NPE3-3 in cells resulted in expected splicing of the CD44 control minigene. We also discovered that NPE3-3-overexpressing clones produced a novel, previously unrecognized, alternatively spliced variant of ER (termed ERV), which had a molecular size of 37 kDa composed of only exons 1, 2, 7, and 8. ERV was expressed and sequestered in the cytoplasm in MCF-7 cells stably overexpressing NPE3-3, suggesting its involvement in nongenomic hormone signaling. NPE3-3 clones exhibited up-regulation of ERK1/2 signaling, cyclin D1, and cathepsin D and enhanced tumor cell proliferation, migration, and tumorigenicity. Moreover, direct expression of the ERV in breast cancer cells stimulated ERK1/2 up-regulation and cyclin D1 expression. We found that ERV physically interacted with MAPK kinase (MEK)-1/2, and thus, an ERV and MEK1/2 complex could lead to the activation of the ERK1/2 pathway. Interestingly, NPE3-3 was up-regulated in human breast tumors. These findings revealed a role for NPE3-3 in alternative splicing and suggest that ER is a physiological target of NPE3-3, leading to a constitutive nongenomic signaling pathway in breast cancer cells.

  S.R. Joshi , R. Kumar , P. Saikia , R.K. Bhagobaty and S. Thokchom
  The study was done for a complete year on monthly basis and the differences on the various parameters selected were measured. Significantly higher number of fungi and bacteria were recorded in non-roadside soil then the effected roadside soil. Rate of soil respiration, enzymes activity also followed a similar trend. Other physicochemical characteristics as soil moisture, soil pH, soil temperature and soil carbon revealed similar variations in two conditions. Microbial population and enzyme activities like dehydrogenase, urease and phosphatase were positively correlated (p<0.05) to the edaphic properties of the soil. Microbial populations, soil respiration, enzymes activity and other physicochemical characteristics were determined from the sub-tropical forest soils of roadside (disturbed) and non-roadside (undisturbed) conditions to compare and the adverse effects of anthropogenic activities and roadside vehicular pollution in the busy national highway. The study site is devoid of human settlement and was once a pristine environment prior to the construction of highway through the forest. An average of 10,000 to 12,000 vehicles plies through the road per day.
  P. R Molli , D. Q Li , R Bagheri Yarmand , S. B Pakala , H Katayama , S Sen , J Iyer , J Chernoff , M. Y Tsai , S. S Nair and R. Kumar

In addition to its function as an Arp2/3 complex subunit, Arp1cb interacts with and stimulates Aurora A at centrosomes, functioning in cell cycle progression.

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