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Articles by Qing Chen
Total Records ( 4 ) for Qing Chen
  Han-Wu Zhang , Xiao-Rong Wang , Bi-Feng Zhong , Wu-Feng Xia , Yuan Liu and Qing Chen
  Genetic relationships among 17 bramble cultivars introduced abroad and 11 wild excellent Rubus germplasms from China were analyzed by RAPD markers. A total of 500 DNA bands were amplified by 22 primers and 490 out of 500 bands (98%) were polymorphic. The average number of polymorphic DNA bands amplified by each primer was 22.28. All materials could be distinguished by RAPD markers with 0.687-0.964 genetic similarity coefficients. According to the data, a dendrogram of genetic relationship, which was constructed using the UPGMA method, showed that all the tested cultivars and wild species (varieties) were classified into four groups. The present results verify that the close genetic relationship between ten raspberry cultivars and seven excellent wild germplasms from section (subgenus) Idaeobatus of the genus Rubus, while a little far genetic relationship between ten raspberry cultivars and seven blackberry cultivars. There was a far genetic relationship between seven wild germplasms from section (subgenus) Idaeobatus and four wild germplasms from section (subgenus) Malachobatus. The two cultivated bramble groups (raspberry and blackberry) could be completely distinguished, but it was failed to distinguish red raspberry with even red raspberry in raspberry group.
  Ma Zhao-cai , Qing Chen and You-liang Zheng
  He Song , Yang Lin , Zhaorong Dong , Qing Chen and Jinchuang Wang
  Base cations input-output budgets are usually considered as important indicators for the sustainability of agricultural production systems. In vegetable production systems, excessive inputs of manure and irrigation water may disturb the base cations budgets. A case study on the base cation input-output budgets of greenhouse was carried out in Shouguang, a typical greenhouse vegetable production region in Northern China. From Feb, 2009 to Jan, 2010, researchers evaluated base cation input-output budgets and identified the most important input and output pathways of base cations under three urea management levels. The balances of base cations showed net surpluses of 5.2-34.1 kmol 1/2 Ca2+, 25.2-30.0 kmol 1/2 Mg2+, 12.0-15.9 kmol K+ and 22.1-25.1 kmol Na+/ha/year under different Nitrogen (N) levels. Different N levels did not produce significantly different effects on the budgets of base cations. The major contributor to Ca2+, Mg2+ and Na+ surpluses was irrigation water whereas the main contributor to K+ surplus was the overuse of potassium (K) fertilizer. Chicken manure also was an important contributor to Ca2+, Mg2+ and K+ surpluses but not to Na+ surplus. The surpluses have changed the composition of soil exchangeable base cations and pose a potential threat to soil quality and crop growth. Furthermore, surplus K in soil can be lost through leaching which is a waste of resources.
  Qing Chen , Pengfei Qi , Renlin Xu , James T. Tambong , Zeinab R. Djama and Wei Li
  Pseudomonads are of significant environmental importance as the members including plant and animal/human pathogens, xenobiotic degraders, plant growth promoters and biocontrol agents. Three typing methods were evaluated, utilising 143 strains of fluorescent pseudomonads isolated from maize-oat-alfalfa-alfalfa rotation plots in Canada, to assess their usefulness as tools to study the microbial community diversity. The methods used were repetitive element sequence-based PCR (rep-PCR) utilising BOX and Enterobacterial Repetitive Intergenic Consensus (ERIC) primers, another new PCR method using an engineered polymerase and 10-nucleotide “miniprimers” expands the scope of detectable sequences beyond those detected by standard methods using longer primers and Taq polymerase. Cluster analysis of the results clearly demonstrated the groups can be divided into two big clusters in line with the morphological variation. Rep-PCR proved to be highly discriminatory, interesting tools for the delineation or genotyping of bacterial species. We concluded that a combination of Box-, Eric- and Miniprimer-PCR results is a rapid and reliable alternative for discrimination among P. fluorescens isolates, contributing to epidemiological studies.
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