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Articles by Pudji Astuti
Total Records ( 6 ) for Pudji Astuti
  Syahran Wael , Tri Rini Nuringtyas , Nastiti Wijayanti and Pudji Astuti
  Background and Objective: Clove (Syzygium aromaticum (L.) Merr. and Perry) used in folk medicinal and traditional plant to treat various diseases. This plant is widely used and has commercial value because the content of secondary metabolite compounds. Differences in the compound content of the plant have contributed to the taxonomic position of the plant. There are many varieties of cloves, i.e., zanzibar, sikotok, siputik and raja. This research was aimed to analyze compounds of cloves var. zanzibar, sikotok, siputik, raja and their phylogenetic relationship. Materials and Methods: The material used leaves of Syzygium aromaticum (Linn.) Var. zanzibar, sikotok, siputik and raja was taken from the plantation of Negeri Lima, Maluku, Indonesia. Extraction using maceration method with n-hexane pro-analysis solvent. Chemical compounds were determined by using GC-MS. GC-MS data from 4 varieties of clove plants were analyzed using SPSS version 20 statistical software using cluster analysis based on UPGMA method (unweighted paired group method with arithmetic mean). Results: Varieties zanzibar contained 5 compounds, i.e., eugenol (70.43%), β-caryophyllene (16.79%), α-humulene (3.05%), caryophyllene oxide (2.07%), tetratetracontane (3.12%). Var. raja compounds consisted of 77.24% eugenol, 16.15% β-caryophyllene, 1.52% α-humulene, 5.08% caryophyllene oxide. Var. sikotok consisted of 16.50% β-isoprophylideneglycerol, 48.33% eugenol, 28.80% β-caryophyllene, 2.97% α-humulene, 3.40% caryophyllene oxide. Var. siputik consisted of 80.15% eugenol, 13.44% β-caryophyllene, 1.50% α-humulene, 4.90% caryophyllene oxide. Similarity values are found in var. siputik and raja with a similarity index of 0.25 followed by zanzibar with siputik 11.9 and zanzibar with sikotok 14.34. Conclusion: The highest compound content is 80.15% eugenol found in var. siputik followed by 28.80% β-caryophyllene compound in var.sikotok, α-humulene compound 3.05% in var.zanzibar and 5.08% caryophyllene oxide compound in var.raja.
  Sabrina M. Pratama , Mei Syafriadi , Pudji Astuti and Dwi Merry CH.R
  The objective was to determine the effect of thymoquinone towards socket bone formation process post extraction in diabetic-induced rats. About 27 male Wistar rats were induced diabetes by using streptozotocin (50 mg/b.w) intravenously. Rats with blood glucose level $250 mg/dL were considered DM and were divided into Thymoquinone (Tq), Metformin (Met) and Control (C) group. The treatments were given start from the 1st day of DM. On the 7th day after treatment, its mandibular left first molar tooth was extracted. On the 3rd, 7th and 10th day post extraction, 3 rats of each group were euthanized. Blood glucose level was measured before and after diabetes induction, before extraction and euthanized. Paraffin embedding tissue processing method and HE and Mallory’s Trichrome staining were used to observing the socket bone healing. Tq treatment significantly decreased the blood glucose level on the 10th day post extraction although, it was still above the normal range (334.3±65.9 mg/dL). Histologically, socket bone healing process in Tq group showed a continuously formation of provisional matrix, woven bone and lamellar bone on the 3rd, 7th and 10th-day post extraction compared to Met and C group. The antidiabetic properties of Tq may well-related to socket bone healing and moreover, Tq also may stimulates the osteoblasts activity which the function was essential in bone formation process. Treatment with Tq is effective to prevent the delay of socket bone healing in socket post extraction in diabetic rats by lowering blood glucose level and by stimulating the osteoblasts activity.
  Harlita , N.H. Niken Satuti , Mammed Sagi and Pudji Astuti
  Cashew plant (Anacardium occidentale L.) is a crop producing cashew nut shell that contain phenolic compounds such as lacquer oil (cashew nut shell liquid) which can be used for many studies. This study was conducted to determine the potency of acute toxicity (LD50) of cashew nut shell extract on female albino Wistar rats using Weil method. Twenty rats used in this study. The rats was divided into five groups, each consist of four rats after acclimatization. Each group was given the extract of cashew nut shell orally (force-fed). The amount of cashew nut shell extract that were given to group I, II, III and IV were 2.5, 25, 250 and 2,500 mg kg–1 b.wt., respectively, while group V were given 0.5% sodium carboxyl methyl cellulose (CMCNa) solution. Clinical symptoms were observed 24 h after the administration of extract include behavioral changes i.e., licking, scratching, twitching, tremors, wrihing, reactivity to stimuli, cerebral and spinal reflexes, secretions, breath, skin, hair and death. Probit analysis using Weil method was used as an effective dose. The results showed that the potency for acute toxicity (LD50) of cashew nut shell extract was 2,018 mg kg–1 which classified as moderately toxic category. The administration of extract also causes behavioral changes in animal including passivity and mucus secretion. All doses of the extract did not affect the development body weight and the weight of organs (spleen, liver, heart, kidneys and lungs) in female rats.
  Meti Indrowati , Rarastoeti Pratiwi , Rumiyati and Pudji Astuti
  Background and Objective: Information about the Artocarpus altilis leaf as an antidiabetic associated with the active compounds Gamma Amino Butyric Acid (GABA) is still limited. This study was conducted to determine the effects of ethanolic extract of A. altilis leaves decoction and GABA on blood glucose levels and insulin expression of beta-cells in streptozotocin-induced diabetic rats. Material and Methods: This study was done by using completely randomized design and male Sprague Dewley rats. The rats were devided into normal control group and diabetic rats groups. Levels of bood glucose were measured using strip rapid test. The insulin expression in beta-cells was assessed using immunohistochemistry. Quantitative data were analyzed using ANOVA at 5% confidence level. Results: The result indicated that 50 mg k–1 b.wt., GABA, 400 and 800 mg k–1 b.wt., ethanolic extract of A. altilis leaves decreased the level of blood glucose and increased the insulin expression in pancreas beta-cells. Conclusion: The GABA and ethanolic extract of A. altilis leaves with a minimum dose of 400 mg k–1 b.wt., can be used as an antidiabetic. Pancreas is the target organ was affected by GABA and A. altilis leaves as antidiabetic agents. Results of this study may support the development of research on the potency of GABA in natural materials as antidiabetic particularly type 1 diabetes.
  Sarmin , Irkham Widiyono , Pudji Astuti and Prabowo Purwono Putro
  Background: The ability of ruminants to undergo metabolic and endocrine adaptation to feeding restrictions and refeeding varies depending on the species and physiological characteristics, the age at which the feed restriction and refeeding are imposed, the rate and length of the feed restriction and refeeding, the physiological responses of various body parts to the feed restriction and refeeding conditions, sex and breed. Objective: The objective of this study was to evaluate the metabolic rates and endocrine responses of Kacang goats, Indonesian-bred ruminants, during the restricted feeding and refeeding periods. Methodology: Nine 1.5-2.5 year-old male goats were subjected to feed adaptation for the first 60 days. Then, the goats were subjected to three-level feed restriction for the second 60 days. The goats received 40, 50 or 60% feed restriction of dry fodder. The goats allocated to the 40, 50 or 60% feed restriction groups were referred to as group 1, 2 and 3, respectively. For the final 60 days, the goats underwent 100% refeeding with dry fodder. The weights of the ruminants were measured and blood samples were obtained at the end of the feed adaptation, feed restriction and refeeding. Results: At the end of the 60-day restriction, the three goats in group 3 were less than the baseline of Average Daily Gain (ADG), while the six other goats in groups 1 and 2 were still greater than the baseline of ADG, although there were no significant differences among the groups (p>0.05). The feed restriction increased lipolysis in adipose tissue and protein mobilization, non-esterified fatty acids (NEFAs) and albumin as the carrier of NEFA, creatinine and total protein in serum. Nonetheless, there were no differences among the three groups (p>0.05). Thyroid hormones (T3 and T4) as lipolytic hormones were ignored because their rates were lower than the baseline. Lipolytic responses in the form of High Density Lipoprotein (HDL) occurred in the three groups and there were significant differences among the three groups (p>0.05), indicating that triglycerides exported as Low Density Lipoprotein (LDL) were also not different among the three groups (p>0.05). This finding showed that there were no LDL secretion differences among the three groups. The two metabolic ketogenic effects, namely, Blood Urea Nitrogen (BUN) and glucose showed that BUN decreased to less than the baseline in group 2, although there were no significant differences among the groups (p>0.05). The glucose rate in group 2 was the highest among the groups (p<0.05), likely because of the low catabolism of labile protein deposits in group 2. After the refeeding was completed, the ADG in groups 2 and 3 increased significantly (p<0.05). In contrast, the ADG in group 1 did not increase significantly (p>0.05). Lipolysis continued to occur after the refeeding, with high rates of NEFA and albumin in the three groups without any differences among the groups (p>0.05). The lipolysis of hormones T3 and T4 was ignored, with both hormones remaining low. The rates of HDL and triglycerides exported as LDL were also constant and were not different among the three groups (p>0.05). Creatinine decreased significantly in the three groups (p<0.05) and there were no differences among the groups. The total protein increased significantly (p<0.5), but there were no differences among the three groups. The BUN metabolite did not change after refeeding was imposed on the three groups (p>0.05), although the BUN metabolite in group 1 tended to be higher than that in group 2. The glucose rate increased significantly in all three of the groups (p<0.05), although none of them reached the baseline. The ruminants in group 2 possessed the lowest glucose rate (p<0.05). Conclusion: A group of goats with a 50% restriction level of dry fodder tended to use ketones as the source of energy by suppressing protein degradation during feed restriction. The responses of ruminants to refeeding took the forms of continued lipolysis and increased protein degradation.
  Irkham Widiyono , Sarmin , Prabowo Purwono Putro and Pudji Astuti
  Background and Objective: Nutritional status of livestock has impact on growth performance and development of reproductive organs. Testicular mass, semen characteristics, spermatogenesis and reproductive hormones can be influenced by feeding levels. This study was conducted to evaluate semen characteristics of Kacang goats given various feeding levels for 24 weeks. Methodology: Nine adult male Kacang goats were divided into 3 feeding groups (G1, G2 and G3). After a 2 week adaptation period, all three groups of goats were fed fully for 8 weeks (full feeding). Then the goats were fed an amount that was 60% (G1), 50% (G2) or 40% (G3) of full feeding levels for the next 8 weeks (restricted feeding). Finally, the goats were refed fully for the last 8 weeks (re-alimentation). Semen samples were collected using an artificial vagina at the end of each feeding period. Results: Full feeding followed by restricted feeding and resumption of full feeding had no significant effects on the ejaculate volume, sperm cell concentration, sperm cell motility and proportion of live sperm values in Kacang goats. Semen parameters were maintained in the physiological range that was normal for Kacang goats. Conclusion: Kacang goats have a good capacity to preserve semen quality at various levels of feeding.
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