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Articles by Prabhat Kumar Mandal
Total Records ( 5 ) for Prabhat Kumar Mandal
  Sang-Keun Jin , Prabhat Kumar Mandal , Il-Suk Kim and Suk-Nam Kang
  This study was conducted to see the effect of packaging methods and refrigerated storage on the quality of dry-cured pork neck. A dry-cured product was developed from pork neck by modifying the conventional procedure. The cured slices were stored for 90 days under Vacuum Packaging (VP), packaging with 100% N2 (NP) and packaging with 20% CO2+80% N2 (MP) at 4°C. There was no significant difference of pH and shear force values between the packaging methods and due to storage up to 90 days. Moisture content and water activity reduced significantly (p<0.05) in all packaging methods with the increase of storage periods except between 30 and 60 days. TBARS values increased significantly with the increase of storage days and the value decreased significantly in VP sample compared to the NP and MP samples at 30 day of storage. There was no significant difference in the microbial quality (TPC, LAB, Coliforms) between the methods of packaging and due to the storage days. There was no significant difference in the sensory quality between the methods of packaging, however, the quality reduced significantly with the increase of storage days. In general, there were no significant differences between the three packaging systems on the quality of dry cured pork, therefore, we recommend vacuum packaging for this product, since it is more economic and convenient.
  Oyungerel Baatartsogt , Prabhat Kumar Mandal , Hee-kyong Lim , Chi-Ho Lee , Jun-heon Lee and Kangduk Choi
  To understand the pathogenesis of PTA we studied the gene expression profiles of synovial cells of knee using gene chip analysis. PTA was induced by transection of the Anterior Cruciate Ligament (ACL) of left knee in 3 piglets. After sacrificing the piglet at 8 weeks, synovial cells were used for gene expression profiling using Affimetrix GeneChip. Affimetrix GeneChip Operating Software and Robust Multi-array Analysis (RMA) were used to identify differentially expressed genes. Expressions of selected genes were verified by RT-PCR. Histopathologic examination at 8 weeks showed overt chronic inflammation indicating the development of PTA. Through genome analyses it was observed that 87 known genes were up-regulated and 76 known genes were down regulated. By analyzing gene expression, we found differential expression of genes related to inflammatory and immune response, lipid binding, cell adhesion, growth activity and muscle development. Some of the important genes related to inflammatory and immune response are FAS, MCP-1, IL-18, IL-1α, IL-6, IL-7, OAS1, TLR4, MX2. RT-PCR result has confirmed the expression of selected genes (IL 6, IL 18, FABP 4, LPL and PLTP) in total RNA isolated from synovial cells. The genome analysis of synovial cells provided us new candidate molecules related to inflammation and immunity which may be useful to understand the pathogenesis of PTA.
  Go-Eun Hong , Prabhat Kumar Mandal , Ki-Won Lim and Chi-Ho Lee
  Isoflavone aglycone content increases after fermentation in soy pulp which is known for prevention of postmenopausal disease. The purpose of this study was to establish optimum fermentation conditions and suitable culture for mass production of aglycone using black soybean pulp, a by-product of soybean processing. Black soybean pulp was fermented up to 48 h using Lactobacillus acidophilus (LA) and Bacillus subtilis (BS) culture. Samples were collected every 12 h to measure isoflavones (daidzin and genistin and daidzein and genistein) using High-performance Liquid Chromatography (HPLC) and β-glucosidase activity using spectrophotometry. Isoflavone aglycone content was verified by Liquid Chromatography-tandem Mass Spectrometry (LC-MS/MS) analysis. Genistein concentrations in black soybean pulp after 12 and 24 h of fermentation with L. acidophilus were 6.8 and 7.2 fold, respectively, compared to controls. Genistein concentrations in black soybean pulp after 12 and 24 h of fermentation with B. subtilis were 6.8 and 6.9 fold greater than controls. β-glucosidase activity was highest in the black soybean pulp fermented for 12 h with L. acidophilus. Black soybean pulp fermented for 12 h with L. acidophilus was most efficient to produce isoflavone aglycone.
  Eun-Jeong Son , Uttam Kumar Pal , Prabhat Kumar Mandal , Go-Eun Hong , Soo-Ki Kim and Chi-Ho Lee
  This study was performed to determine the hypolipidaemic effects of Allium tuberosum Rottl. (ATR), fermented Allium tuberosum Rottl. (FATR) and processed sulfur. Thirty male Wistar rats were divided into five groups: (i) CON (control, normal diet); (ii) HFD (high fat diet); (iii) HS (HFD+processed sulfur); (iv) AS (HFD+ATR+processed sulfur) and (v) FAS (HFD+FATR+ processed sulfur). Animals were fed the diets for six weeks. Body weights of the animals were recorded in the beginning and end of experiment. The animals were sacrificed, organ and tissue weights recorded and blood and liver samples were analyzed for various biochemical parameters. Body weight gain in the AS group was significantly (p<0.05) lower than the other groups. Spleen and kidney weights in FAS group were significantly (p<0.05) higher than the other groups. The weight of epididymal adipose tissue in AS group was significantly (p<0.05) lower than that of HFD group. The weight of retroperitoneal fat in HFD groups was significantly (p<0.05) higher than other groups. The concentration of aspartate amino transferase (AST) was significantly (p<0.05) higher in HFD group compared to HS, AS and FAS groups. Total liver lipid contents in the HFD group was significantly (p<0.05) higher than the other groups. AS and FAS group rats had significantly (p<0.05) lower triglyceride and total cholesterol compared to HFD group. All together, these results suggest that FATR and processed sulfur may play an important role in reducing body fat deposition and altering the serum and liver lipid profile in rats.
  Sudip Kumar Das , Subhasish Biswas and Prabhat Kumar Mandal
  The present study was envisaged to standardize a traditional meat product of Purulia District, West Bengal, India, chevon pithe (meat cake), characterize its quality and to study shelf-life in refrigerated storage (4±1°C). The formulation and procedure was standardized through several preliminary trials. The traditionally made (TCP) and laboratory made ‘Chevon-Pithe’ (LCP) were compared for different physicochemical, sensory and microbial quality. The cooking yield (%), moisture retention (%), fat retention (%) and water holding capacity (%) of cooked LCP samples were found to be 76.62±1.43, 48.85±1.19, 88.14±2.12 and 42.55±1.17, respectively. Moisture (%), protein (%), ether extract (%) and total ash (%) of LCP samples were found as 64.31±0.83, 10.59±0.34, 13.71±1.28, 11.52±0.86 and 2.86±0.71, respectively and the cholesterol (mg %) content of LCP sample was 62.22±1.78. TBA value (mg malonaldehyde kg-1) of cooked LCP samples was 0.368±0.024 at 0 day and 1.037±0.063 at 14th day. Peroxide value (meq kg-1) and free fatty acid (% oleic acid) of cooked LCP samples were found to be 1.160±0.129 and 0.217±0.092 at 0 day and 6.290±0.163 and 3.998±0.178 at 14th day, respectively. The study revealed that the LCP retained its acceptability up to 14th day of refrigerated storage (4±1°C). Traditionally made one also got sufficient consumer attention not very differently, throughout the storage. Based on the results it was concluded that ‘chevon-pithe’ can be commercially prepared and marketed as a value added meat product.
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